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Superporous agarose monoliths as mini-reactors in flow injection systems

Nandakumar, Madayi P. ; Pålsson, Eva ; Gustavsson, Per-Erik LU ; Larsson, Per-Olof LU and Mattiasson, Bo LU (2000) In Bioseparation 9(4). p.193-202
Abstract
A new type of agarose material, superporous agarose, was used as a
support material in an analytical system designed for monitoring of
bioprocesses with respect to metabolites and intracellular enzymes. The
superporous agarose was used in the form of miniaturised gel plug
columns (15×5.0 mM I.D. monolithic gel bed). The gel plugs were designed
to have one set of very large pores (about 50 μm in diameter) through
which cells, cell debris and other particulate contaminants from the
bioreactor could easily pass. The material also had normal diffusion
pores (300 Å) characteristic of all agarose materials, providing ample
surface for covalent attachment of antibodies and enzymes used in the
... (More)
A new type of agarose material, superporous agarose, was used as a
support material in an analytical system designed for monitoring of
bioprocesses with respect to metabolites and intracellular enzymes. The
superporous agarose was used in the form of miniaturised gel plug
columns (15×5.0 mM I.D. monolithic gel bed). The gel plugs were designed
to have one set of very large pores (about 50 μm in diameter) through
which cells, cell debris and other particulate contaminants from the
bioreactor could easily pass. The material also had normal diffusion
pores (300 Å) characteristic of all agarose materials, providing ample
surface for covalent attachment of antibodies and enzymes used in the
analytical sequence. The superporous agarose gel plug columns were
characterised with respect to flow properties and handling of heavy cell
loads as well as dispersion of injected samples (a Bodenstein number of
about 40 was observed with acetone tracer at a flow rate of 1 ml min−1).
To evaluate the practical performance of the superporous gel plug
columns, two applications were studied: (1) on-line determination of
glucose in cultivation broth (gel plug with immobilized glucose oxidase)
and (2) immunochemical quantification of intracellular β-galactosidase
in E. coli (gel plug with lysozyme to achieve cell lysis and gel plug with antibodies against β-galactosidase). (Less)
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author
; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Glucose analysis, Cultivation broth, Lysozyme gel
in
Bioseparation
volume
9
issue
4
pages
193 - 202
publisher
Springer
external identifiers
  • scopus:0034468126
ISSN
1573-8272
DOI
10.1023/A:1008117827057
language
English
LU publication?
yes
id
85fbe3ba-0664-449b-996d-ec74228674e7
date added to LUP
2024-05-17 21:38:50
date last changed
2024-05-27 13:53:59
@article{85fbe3ba-0664-449b-996d-ec74228674e7,
  abstract     = {{A new type of agarose material, superporous agarose, was used as a <br>
support material in an analytical system designed for monitoring of <br>
bioprocesses with respect to metabolites and intracellular enzymes. The <br>
superporous agarose was used in the form of miniaturised gel plug <br>
columns (15×5.0 mM I.D. monolithic gel bed). The gel plugs were designed<br>
 to have one set of very large pores (about 50 μm in diameter) through <br>
which cells, cell debris and other particulate contaminants from the <br>
bioreactor could easily pass. The material also had normal diffusion <br>
pores (300 Å) characteristic of all agarose materials, providing ample <br>
surface for covalent attachment of antibodies and enzymes used in the <br>
analytical sequence. The superporous agarose gel plug columns were <br>
characterised with respect to flow properties and handling of heavy cell<br>
 loads as well as dispersion of injected samples (a Bodenstein number of<br>
 about 40 was observed with acetone tracer at a flow rate of 1 ml min<sup>−1</sup>).<br>
 To evaluate the practical performance of the superporous gel plug <br>
columns, two applications were studied: (1) on-line determination of <br>
glucose in cultivation broth (gel plug with immobilized glucose oxidase)<br>
 and (2) immunochemical quantification of intracellular β-galactosidase <br>
in <i>E. coli</i> (gel plug with lysozyme to achieve cell lysis and gel plug with antibodies against β-galactosidase).}},
  author       = {{Nandakumar, Madayi P. and Pålsson, Eva and Gustavsson, Per-Erik and Larsson, Per-Olof and Mattiasson, Bo}},
  issn         = {{1573-8272}},
  keywords     = {{Glucose analysis; Cultivation broth; Lysozyme gel}},
  language     = {{eng}},
  number       = {{4}},
  pages        = {{193--202}},
  publisher    = {{Springer}},
  series       = {{Bioseparation}},
  title        = {{Superporous agarose monoliths as mini-reactors in flow injection systems}},
  url          = {{http://dx.doi.org/10.1023/A:1008117827057}},
  doi          = {{10.1023/A:1008117827057}},
  volume       = {{9}},
  year         = {{2000}},
}