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Kinetic analysis of amyloid formation

Meisl, Georg; Michaels, Thomas C.T.; Linse, Sara LU and Knowles, Tuomas P.J. (2018) In Methods in Molecular Biology 1779. p.181-196
Abstract

The formation of amyloid fibrils is a central phenomenon in the progressive pathology of many neurodegenerative diseases, as well as in the fabrication of functional materials. Several different molecular processes acting in concert are responsible for the formation of amyloid fibrils from monomeric protein in solution. Here, we describe a method to determine which microscopic processes drive the overall formation of fibrils by using chemical kinetics in combination with systematic experimental datasets analysed in a global manner. We outline general concepts for obtaining suitable kinetic data and detail the key stages of data analysis, from quality control to the verification of a specific mechanism of aggregation.

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author
organization
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
keywords
Amyloid fibrils, Kinetic modeling, Molecular-level mechanism, Protein aggregation, Self-assembly
host publication
Methods in Molecular Biology
series title
Methods in Molecular Biology
volume
1779
pages
16 pages
publisher
Humana Press
external identifiers
  • scopus:85048513546
ISSN
1064-3745
DOI
10.1007/978-1-4939-7816-8_12
language
English
LU publication?
yes
id
861f9621-8af7-419e-b7d3-34c3508f21df
date added to LUP
2018-07-03 11:50:30
date last changed
2019-05-21 04:08:19
@inbook{861f9621-8af7-419e-b7d3-34c3508f21df,
  abstract     = {<p>The formation of amyloid fibrils is a central phenomenon in the progressive pathology of many neurodegenerative diseases, as well as in the fabrication of functional materials. Several different molecular processes acting in concert are responsible for the formation of amyloid fibrils from monomeric protein in solution. Here, we describe a method to determine which microscopic processes drive the overall formation of fibrils by using chemical kinetics in combination with systematic experimental datasets analysed in a global manner. We outline general concepts for obtaining suitable kinetic data and detail the key stages of data analysis, from quality control to the verification of a specific mechanism of aggregation.</p>},
  author       = {Meisl, Georg and Michaels, Thomas C.T. and Linse, Sara and Knowles, Tuomas P.J.},
  issn         = {1064-3745},
  keyword      = {Amyloid fibrils,Kinetic modeling,Molecular-level mechanism,Protein aggregation,Self-assembly},
  language     = {eng},
  month        = {01},
  pages        = {181--196},
  publisher    = {Humana Press},
  series       = {Methods in Molecular Biology},
  title        = {Kinetic analysis of amyloid formation},
  url          = {http://dx.doi.org/10.1007/978-1-4939-7816-8_12},
  volume       = {1779},
  year         = {2018},
}