Evidence for prejunctional GABAB receptors mediating inhibition of ovarian follicle contraction induced by nerve stimulation
(1986) In European Journal of Pharmacology 122(1). p.9-123- Abstract
The motor effects of gamma-aminobutyric acid (GABA) on the bovine ovarian follicle were studied in vitro using strips from follicle walls. Electrical field stimulation of nerves in the preparation, secured by tetrodotoxin blockade, caused a contraction that was almost totally abolished by phentolamine and only slightly affected by atropine. This mainly adrenergic neurogenic response was inhibited by GABA in a dose-dependent way. The GABAA-receptor antagonists, bicuculline and picrotoxin, did not affect the GABA action whereas the GABAB-receptor antagonist, homotaurine, significantly inhibited the GABA effect. The GABAA-receptor agonist, muscimol, did not affect the contractile response while the GABAB-receptor agonist, baclofen,... (More)
The motor effects of gamma-aminobutyric acid (GABA) on the bovine ovarian follicle were studied in vitro using strips from follicle walls. Electrical field stimulation of nerves in the preparation, secured by tetrodotoxin blockade, caused a contraction that was almost totally abolished by phentolamine and only slightly affected by atropine. This mainly adrenergic neurogenic response was inhibited by GABA in a dose-dependent way. The GABAA-receptor antagonists, bicuculline and picrotoxin, did not affect the GABA action whereas the GABAB-receptor antagonist, homotaurine, significantly inhibited the GABA effect. The GABAA-receptor agonist, muscimol, did not affect the contractile response while the GABAB-receptor agonist, baclofen, imitated the action of GABA. On the other hand, GABA had no direct contractile or relaxing effect on the follicle strips nor did it interfere with the contractile response induced by noradrenaline or acetylcholine. The findings suggest that activation of prejunctional GABAB receptors inhibits transmitter release from mainly adrenergic nerves associated with the follicle, thereby affecting nerve-mediated tension in the follicle wall.
(Less)
- author
- Kannisto, P LU ; Owman, C LU ; Walles, B and Schmidt, Gunther
- organization
- publishing date
- 1986-03-11
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Animals, Atropine/pharmacology, Baclofen/pharmacology, Bicuculline/pharmacology, Cattle, Electric Stimulation, Female, In Vitro Techniques, Muscle Contraction/drug effects, Muscle, Smooth/physiology, Ovarian Follicle/innervation, Phentolamine/pharmacology, Receptors, GABA-A/physiology, Synaptic Membranes/physiology, Taurine/analogs & derivatives, Tetrodotoxin/pharmacology, gamma-Aminobutyric Acid/pharmacology
- in
- European Journal of Pharmacology
- volume
- 122
- issue
- 1
- pages
- 9 - 123
- publisher
- Elsevier
- external identifiers
-
- pmid:3007173
- scopus:0022558918
- ISSN
- 0014-2999
- DOI
- 10.1016/0014-2999(86)90167-6
- language
- English
- LU publication?
- yes
- id
- 863197db-6e14-4a78-ba13-5c2b011dde15
- date added to LUP
- 2019-12-03 22:22:51
- date last changed
- 2024-01-02 01:38:18
@article{863197db-6e14-4a78-ba13-5c2b011dde15,
abstract = {{<p>The motor effects of gamma-aminobutyric acid (GABA) on the bovine ovarian follicle were studied in vitro using strips from follicle walls. Electrical field stimulation of nerves in the preparation, secured by tetrodotoxin blockade, caused a contraction that was almost totally abolished by phentolamine and only slightly affected by atropine. This mainly adrenergic neurogenic response was inhibited by GABA in a dose-dependent way. The GABAA-receptor antagonists, bicuculline and picrotoxin, did not affect the GABA action whereas the GABAB-receptor antagonist, homotaurine, significantly inhibited the GABA effect. The GABAA-receptor agonist, muscimol, did not affect the contractile response while the GABAB-receptor agonist, baclofen, imitated the action of GABA. On the other hand, GABA had no direct contractile or relaxing effect on the follicle strips nor did it interfere with the contractile response induced by noradrenaline or acetylcholine. The findings suggest that activation of prejunctional GABAB receptors inhibits transmitter release from mainly adrenergic nerves associated with the follicle, thereby affecting nerve-mediated tension in the follicle wall.</p>}},
author = {{Kannisto, P and Owman, C and Walles, B and Schmidt, Gunther}},
issn = {{0014-2999}},
keywords = {{Animals; Atropine/pharmacology; Baclofen/pharmacology; Bicuculline/pharmacology; Cattle; Electric Stimulation; Female; In Vitro Techniques; Muscle Contraction/drug effects; Muscle, Smooth/physiology; Ovarian Follicle/innervation; Phentolamine/pharmacology; Receptors, GABA-A/physiology; Synaptic Membranes/physiology; Taurine/analogs & derivatives; Tetrodotoxin/pharmacology; gamma-Aminobutyric Acid/pharmacology}},
language = {{eng}},
month = {{03}},
number = {{1}},
pages = {{9--123}},
publisher = {{Elsevier}},
series = {{European Journal of Pharmacology}},
title = {{Evidence for prejunctional GABAB receptors mediating inhibition of ovarian follicle contraction induced by nerve stimulation}},
url = {{http://dx.doi.org/10.1016/0014-2999(86)90167-6}},
doi = {{10.1016/0014-2999(86)90167-6}},
volume = {{122}},
year = {{1986}},
}