Abdominal organ perfusion and inflammation in experimental sepsis: a magnetic resonance imaging study.
Marchesi, Silvia LU
; Ortiz-Nieto, Francisco
; Ahlgren, Kerstin M
; Roneus, Agneta
; Feinstein, Ricardo
; Lipcsey, Miklos
; Larsson, Anders
; Ahlström, Håkan
and Hedenstierna, Göran
(2019)
In American Journal of Physiology: Gastrointestinal and Liver Physiology
316(1).
p.19-187
- Abstract
- Diffusion-weighted magnetic resonance imaging (DW-MRI) uses water as contrast and enables the study of perfusion in many organs simultaneously in situ. We used DW-MRI in a hypodynamic sepsis model, comparing abdominal organ perfusion with global hemodynamic measurements and inflammation. Sixteen anesthetized piglets were randomized into 3 groups: 2 intervention (sepsis) groups: HighMAP (mean arterial pressure, MAP > 65 mmHg) and LowMAP (MAP between 50 and 60 mmHg), and a Healthy Control group (HC). Sepsis was obtained with endotoxin and the desired MAP maintained with norepinephrine. After 6 h, DW-MRI was performed. Acute inflammation was assessed with IL-6 and TNFα in abdominal organs, ascites, and blood and by histology of intestine... (More)
- Diffusion-weighted magnetic resonance imaging (DW-MRI) uses water as contrast and enables the study of perfusion in many organs simultaneously in situ. We used DW-MRI in a hypodynamic sepsis model, comparing abdominal organ perfusion with global hemodynamic measurements and inflammation. Sixteen anesthetized piglets were randomized into 3 groups: 2 intervention (sepsis) groups: HighMAP (mean arterial pressure, MAP > 65 mmHg) and LowMAP (MAP between 50 and 60 mmHg), and a Healthy Control group (HC). Sepsis was obtained with endotoxin and the desired MAP maintained with norepinephrine. After 6 h, DW-MRI was performed. Acute inflammation was assessed with IL-6 and TNFα in abdominal organs, ascites, and blood and by histology of intestine (duodenum). Perfusion of abdominal organs was reduced in the LowMAP group compared with the HighMAP group and HC. Liver perfusion was still reduced by 25% in the HighMAP group compared with HC. Intestinal perfusion did not differ significantly between the intervention groups. Cytokine concentrations were generally higher in the LowMAP group but did not correlate with global hemodynamics. However, cytokines correlated with regional perfusion and, for liver and intestine, also with intra-abdominal pressure. Histopathology of intestine worsened with decreasing perfusion. In conclusion, although a low MAP (≤60 mmHg) indicated impeded abdominal perfusion in experimental sepsis, it did not predict inflammation, nor did other global measures of circulation. Decreased abdominal perfusion partially predicted inflammation but intestine, occupying most of the abdomen, and liver were also affected by intra-abdominal pressure. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/864ee4a7-c6dc-4dd2-930a-1718ab525708
- author
- Marchesi, Silvia
LU
; Ortiz-Nieto, Francisco
; Ahlgren, Kerstin M
; Roneus, Agneta
; Feinstein, Ricardo
; Lipcsey, Miklos
; Larsson, Anders
; Ahlström, Håkan
and Hedenstierna, Göran
- publishing date
- 2019
- type
- Contribution to journal
- publication status
- published
- in
- American Journal of Physiology: Gastrointestinal and Liver Physiology
- volume
- 316
- issue
- 1
- pages
- 19 - 187
- publisher
- American Physiological Society
- external identifiers
-
- scopus:85059238595
- ISSN
- 1522-1547
- DOI
- 10.1152/ajpgi.00151.2018
- language
- English
- LU publication?
- no
- id
- 864ee4a7-c6dc-4dd2-930a-1718ab525708
- date added to LUP
- 2025-03-07 13:32:15
- date last changed
- 2025-03-10 07:49:40
@article{864ee4a7-c6dc-4dd2-930a-1718ab525708,
abstract = {{Diffusion-weighted magnetic resonance imaging (DW-MRI) uses water as contrast and enables the study of perfusion in many organs simultaneously in situ. We used DW-MRI in a hypodynamic sepsis model, comparing abdominal organ perfusion with global hemodynamic measurements and inflammation. Sixteen anesthetized piglets were randomized into 3 groups: 2 intervention (sepsis) groups: HighMAP (mean arterial pressure, MAP > 65 mmHg) and LowMAP (MAP between 50 and 60 mmHg), and a Healthy Control group (HC). Sepsis was obtained with endotoxin and the desired MAP maintained with norepinephrine. After 6 h, DW-MRI was performed. Acute inflammation was assessed with IL-6 and TNFα in abdominal organs, ascites, and blood and by histology of intestine (duodenum). Perfusion of abdominal organs was reduced in the LowMAP group compared with the HighMAP group and HC. Liver perfusion was still reduced by 25% in the HighMAP group compared with HC. Intestinal perfusion did not differ significantly between the intervention groups. Cytokine concentrations were generally higher in the LowMAP group but did not correlate with global hemodynamics. However, cytokines correlated with regional perfusion and, for liver and intestine, also with intra-abdominal pressure. Histopathology of intestine worsened with decreasing perfusion. In conclusion, although a low MAP (≤60 mmHg) indicated impeded abdominal perfusion in experimental sepsis, it did not predict inflammation, nor did other global measures of circulation. Decreased abdominal perfusion partially predicted inflammation but intestine, occupying most of the abdomen, and liver were also affected by intra-abdominal pressure.}},
author = {{Marchesi, Silvia and Ortiz-Nieto, Francisco and Ahlgren, Kerstin M and Roneus, Agneta and Feinstein, Ricardo and Lipcsey, Miklos and Larsson, Anders and Ahlström, Håkan and Hedenstierna, Göran}},
issn = {{1522-1547}},
language = {{eng}},
number = {{1}},
pages = {{19--187}},
publisher = {{American Physiological Society}},
series = {{American Journal of Physiology: Gastrointestinal and Liver Physiology}},
title = {{Abdominal organ perfusion and inflammation in experimental sepsis: a magnetic resonance imaging study.}},
url = {{http://dx.doi.org/10.1152/ajpgi.00151.2018}},
doi = {{10.1152/ajpgi.00151.2018}},
volume = {{316}},
year = {{2019}},
}