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Inhibition of splicing by serine-arginine rich protein 55 (SRp55) causes the appearance of partially spliced HIV-1 mRNAs in the cytoplasm

Tranell, Anna ; Tingsborg, Susanne ; Fenyö, Eva Maria LU and Schwartz, Stefan LU (2011) In Virus Research 157(1). p.82-91
Abstract
We have previously shown that SRp55 inhibits splicing from HIV-1 exon 3, thereby generating partially spliced mRNAs encoding HIV-1 vpr. Here we show that SRp55 also inhibits splicing from HIV-1 exon 5 to generate HIV-1 vpu/env mRNA, albeit with lower efficiency. We also show that inhibition of HIV-1 splicing by SRp55 causes the appearance of partially spliced vpu, env and vpr mRNAs in the cytoplasm. SRp55 could also induce production of extracellular p24gag from a rev-defective HIV-1 provirus. These results indicate that SRp55 aids in the generation of partially spliced and unspliced HIV-1 mRNAs. (C) 2011 Elsevier B.V. All rights reserved.
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author
; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
HIV-1, vpr, SRp55, Splicing
in
Virus Research
volume
157
issue
1
pages
82 - 91
publisher
Elsevier
external identifiers
  • wos:000289864600011
  • scopus:79953024872
ISSN
1872-7492
DOI
10.1016/j.virusres.2011.02.010
language
English
LU publication?
yes
id
8655d89e-8339-4122-8bae-c2fe770ee404 (old id 1962857)
date added to LUP
2016-04-01 10:02:36
date last changed
2025-04-04 15:20:57
@article{8655d89e-8339-4122-8bae-c2fe770ee404,
  abstract     = {{We have previously shown that SRp55 inhibits splicing from HIV-1 exon 3, thereby generating partially spliced mRNAs encoding HIV-1 vpr. Here we show that SRp55 also inhibits splicing from HIV-1 exon 5 to generate HIV-1 vpu/env mRNA, albeit with lower efficiency. We also show that inhibition of HIV-1 splicing by SRp55 causes the appearance of partially spliced vpu, env and vpr mRNAs in the cytoplasm. SRp55 could also induce production of extracellular p24gag from a rev-defective HIV-1 provirus. These results indicate that SRp55 aids in the generation of partially spliced and unspliced HIV-1 mRNAs. (C) 2011 Elsevier B.V. All rights reserved.}},
  author       = {{Tranell, Anna and Tingsborg, Susanne and Fenyö, Eva Maria and Schwartz, Stefan}},
  issn         = {{1872-7492}},
  keywords     = {{HIV-1; vpr; SRp55; Splicing}},
  language     = {{eng}},
  number       = {{1}},
  pages        = {{82--91}},
  publisher    = {{Elsevier}},
  series       = {{Virus Research}},
  title        = {{Inhibition of splicing by serine-arginine rich protein 55 (SRp55) causes the appearance of partially spliced HIV-1 mRNAs in the cytoplasm}},
  url          = {{http://dx.doi.org/10.1016/j.virusres.2011.02.010}},
  doi          = {{10.1016/j.virusres.2011.02.010}},
  volume       = {{157}},
  year         = {{2011}},
}