A non-classical route of efficient plant uptake verified with fluorescent nanoparticles and root adhesion forces investigated using AFM
(2020) In Scientific Reports 10(1).- Abstract
Classical plant uptake is limited to hydrophilic or water-dispersible material. Therefore, in order to test the uptake behaviour of hydrophobic particles, here, we tested the fate of hydrophobic particles (oleylamine coated Cu2-xSe NPs (CS@OA)) in comparison to hydrophilic particles (chitosan-coated Cu2-xSe NPs (CS@CH)) by treatment on the plant roots. Surprisingly, hydrophobic CS@OA NPs have been found to be ~ 1.3 times more efficient than hydrophilic CS@CH NPs in tomato plant root penetration. An atomic force microscopy (AFM) adhesion force experiment confirms that hydrophobic NPs experience non-spontaneous yet energetically favorable root trapping and penetration. Further, a relative difference in the... (More)
Classical plant uptake is limited to hydrophilic or water-dispersible material. Therefore, in order to test the uptake behaviour of hydrophobic particles, here, we tested the fate of hydrophobic particles (oleylamine coated Cu2-xSe NPs (CS@OA)) in comparison to hydrophilic particles (chitosan-coated Cu2-xSe NPs (CS@CH)) by treatment on the plant roots. Surprisingly, hydrophobic CS@OA NPs have been found to be ~ 1.3 times more efficient than hydrophilic CS@CH NPs in tomato plant root penetration. An atomic force microscopy (AFM) adhesion force experiment confirms that hydrophobic NPs experience non-spontaneous yet energetically favorable root trapping and penetration. Further, a relative difference in the hydrophobic vs. hydrophilic NPs movement from roots to shoots has been observed and found related to the change in protein corona as identified by two dimensional-polyacrylamide gel electrophoresis (2D-PAGE) analysis. Finally, the toxicity assays at the give concentration showed that Cu2-xSe NPs lead to non-significant toxicity as compared to control. This technology may find an advantage in fertilizer application.
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- author
- Sharma, Sandeep ; Muddassir, Mohd ; Muthusamy, Saraladevi LU ; Vaishnav, Pardeep Kumar ; Singh, Manish LU ; Sharma, Deepak ; Kanagarajan, Selvaraju and Shanmugam, Vijayakumar
- organization
- publishing date
- 2020
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Scientific Reports
- volume
- 10
- issue
- 1
- article number
- 19233
- publisher
- Nature Publishing Group
- external identifiers
-
- pmid:33159139
- scopus:85095681280
- ISSN
- 2045-2322
- DOI
- 10.1038/s41598-020-75685-3
- language
- English
- LU publication?
- yes
- id
- 865dd8c4-357f-4a57-8a24-cf6194c439e9
- date added to LUP
- 2020-11-23 15:01:36
- date last changed
- 2024-04-17 19:31:00
@article{865dd8c4-357f-4a57-8a24-cf6194c439e9,
abstract = {{<p>Classical plant uptake is limited to hydrophilic or water-dispersible material. Therefore, in order to test the uptake behaviour of hydrophobic particles, here, we tested the fate of hydrophobic particles (oleylamine coated Cu<sub>2-x</sub>Se NPs (CS@OA)) in comparison to hydrophilic particles (chitosan-coated Cu<sub>2-x</sub>Se NPs (CS@CH)) by treatment on the plant roots. Surprisingly, hydrophobic CS@OA NPs have been found to be ~ 1.3 times more efficient than hydrophilic CS@CH NPs in tomato plant root penetration. An atomic force microscopy (AFM) adhesion force experiment confirms that hydrophobic NPs experience non-spontaneous yet energetically favorable root trapping and penetration. Further, a relative difference in the hydrophobic vs. hydrophilic NPs movement from roots to shoots has been observed and found related to the change in protein corona as identified by two dimensional-polyacrylamide gel electrophoresis (2D-PAGE) analysis. Finally, the toxicity assays at the give concentration showed that Cu<sub>2-x</sub>Se NPs lead to non-significant toxicity as compared to control. This technology may find an advantage in fertilizer application.</p>}},
author = {{Sharma, Sandeep and Muddassir, Mohd and Muthusamy, Saraladevi and Vaishnav, Pardeep Kumar and Singh, Manish and Sharma, Deepak and Kanagarajan, Selvaraju and Shanmugam, Vijayakumar}},
issn = {{2045-2322}},
language = {{eng}},
number = {{1}},
publisher = {{Nature Publishing Group}},
series = {{Scientific Reports}},
title = {{A non-classical route of efficient plant uptake verified with fluorescent nanoparticles and root adhesion forces investigated using AFM}},
url = {{http://dx.doi.org/10.1038/s41598-020-75685-3}},
doi = {{10.1038/s41598-020-75685-3}},
volume = {{10}},
year = {{2020}},
}