Immunohistochemical detection of the pro-apoptotic Bax∆2 protein in human tissues
(2020) In Histochemistry and Cell Biology 154(1). p.41-53- Abstract
The pro-apoptotic Bax isoform Bax∆2 was originally discovered in cancer patients with a microsatellite guanine deletion (G8 to G7). This deletion leads to an early stop codon; however, when combined with the alternative splicing of exon 2, the reading frame is restored allowing production of a full-length protein (Bax∆2). Unlike the parental Baxα, Bax∆2 triggers apoptosis through a non-mitochondrial pathway and the expression in human tissues was unknown. Here, we analyzed over 1000 tissue microarray samples from 13 different organs using immunohistochemistry. Bax∆2-positive cells were detected in all examined organs at low rates (1-5%) and mainly scattered throughout the connective tissues. Surprisingly, over 70% of normal colon... (More)
The pro-apoptotic Bax isoform Bax∆2 was originally discovered in cancer patients with a microsatellite guanine deletion (G8 to G7). This deletion leads to an early stop codon; however, when combined with the alternative splicing of exon 2, the reading frame is restored allowing production of a full-length protein (Bax∆2). Unlike the parental Baxα, Bax∆2 triggers apoptosis through a non-mitochondrial pathway and the expression in human tissues was unknown. Here, we analyzed over 1000 tissue microarray samples from 13 different organs using immunohistochemistry. Bax∆2-positive cells were detected in all examined organs at low rates (1-5%) and mainly scattered throughout the connective tissues. Surprisingly, over 70% of normal colon samples scored high for BaxΔ2-positive staining. Only 7% of malignant colon samples scored high, with most high-grade tumors being negative. A similar pattern was observed in most organs examined. We also showed that both Baxα and Bax∆2 can co-exist in the same cells. Genotyping showed that the majority of Bax∆2-positive normal tissues contain no G7 mutation, but an unexpected high rate of G9 was observed. Although the underlying mechanism remains to be explored, the inverse correlation of Bax∆2 expression with tissue malignancy suggests that it may have a clinical implication in cancer development and treatment.
(Less)
- author
- Mañas, Adriana LU ; Yao, Qi ; Davis, Aislinn ; Basheer, Sana ; Beatty, Evan ; Zhang, Honghong ; Li, Jiajun ; Nelson, Adam ; Zhang, Huaiyuan and Xiang, Jialing
- publishing date
- 2020-03-21
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Histochemistry and Cell Biology
- volume
- 154
- issue
- 1
- pages
- 41 - 53
- publisher
- Springer
- external identifiers
-
- pmid:32200452
- scopus:85082038482
- ISSN
- 1432-119X
- DOI
- 10.1007/s00418-020-01874-w
- language
- English
- LU publication?
- no
- id
- 86839531-e711-4c60-967b-01e64ad2e1b3
- alternative location
- https://link.springer.com/article/10.1007/s00418-020-01874-w
- date added to LUP
- 2020-04-22 15:50:09
- date last changed
- 2024-03-20 09:05:14
@article{86839531-e711-4c60-967b-01e64ad2e1b3,
abstract = {{<p>The pro-apoptotic Bax isoform Bax∆2 was originally discovered in cancer patients with a microsatellite guanine deletion (G8 to G7). This deletion leads to an early stop codon; however, when combined with the alternative splicing of exon 2, the reading frame is restored allowing production of a full-length protein (Bax∆2). Unlike the parental Baxα, Bax∆2 triggers apoptosis through a non-mitochondrial pathway and the expression in human tissues was unknown. Here, we analyzed over 1000 tissue microarray samples from 13 different organs using immunohistochemistry. Bax∆2-positive cells were detected in all examined organs at low rates (1-5%) and mainly scattered throughout the connective tissues. Surprisingly, over 70% of normal colon samples scored high for BaxΔ2-positive staining. Only 7% of malignant colon samples scored high, with most high-grade tumors being negative. A similar pattern was observed in most organs examined. We also showed that both Baxα and Bax∆2 can co-exist in the same cells. Genotyping showed that the majority of Bax∆2-positive normal tissues contain no G7 mutation, but an unexpected high rate of G9 was observed. Although the underlying mechanism remains to be explored, the inverse correlation of Bax∆2 expression with tissue malignancy suggests that it may have a clinical implication in cancer development and treatment.</p>}},
author = {{Mañas, Adriana and Yao, Qi and Davis, Aislinn and Basheer, Sana and Beatty, Evan and Zhang, Honghong and Li, Jiajun and Nelson, Adam and Zhang, Huaiyuan and Xiang, Jialing}},
issn = {{1432-119X}},
language = {{eng}},
month = {{03}},
number = {{1}},
pages = {{41--53}},
publisher = {{Springer}},
series = {{Histochemistry and Cell Biology}},
title = {{Immunohistochemical detection of the pro-apoptotic Bax∆2 protein in human tissues}},
url = {{http://dx.doi.org/10.1007/s00418-020-01874-w}},
doi = {{10.1007/s00418-020-01874-w}},
volume = {{154}},
year = {{2020}},
}