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Exosomes from human mesenchymal stem cells conduct aerobic metabolism in term and preterm newborn infants

Panfoli, I.; Ravera, S.; Podesta, M.; Cossu, C.; Santucci, L.; Bartolucci, M.; Brusch, M.; Calzia, D.; Sabatini, F. and Bruschettini, Matteo LU , et al. (2016) In FASEB Journal
Abstract
Exosomes are secreted nanovesicles that are able to transfer RNA and proteins to target cells. The emerging role of mesenchymal stem cell (MSC) exosomes as promoters of aerobic ATP synthesis restoration in damaged cells, prompted us to assess whether they contain an extramitochondrial aerobic respiration capacity. Exosomes were isolated from culture medium of human MSC from umbilical cord of ≥37-wk-old newborns or between 28- to 30–wk-old newborns (i.e., term or preterm infants). Characterization of samples was conducted by cytofluorometry. Oxidative phosphorylation capacity was assessed by Western blot analysis, oximetry, and luminometric and fluorometric analyses. MSC exosomes express functional respiratory complexes I, IV, and V,... (More)
Exosomes are secreted nanovesicles that are able to transfer RNA and proteins to target cells. The emerging role of mesenchymal stem cell (MSC) exosomes as promoters of aerobic ATP synthesis restoration in damaged cells, prompted us to assess whether they contain an extramitochondrial aerobic respiration capacity. Exosomes were isolated from culture medium of human MSC from umbilical cord of ≥37-wk-old newborns or between 28- to 30–wk-old newborns (i.e., term or preterm infants). Characterization of samples was conducted by cytofluorometry. Oxidative phosphorylation capacity was assessed by Western blot analysis, oximetry, and luminometric and fluorometric analyses. MSC exosomes express functional respiratory complexes I, IV, and V, consuming oxygen. ATP synthesis was only detectable in exosomes from term newborns, suggestive of a specific mechanism that is not completed at an early gestational age. Activities are outward facing and comparable to those detected in mitochondria isolated from term MSC. MSC exosomes display an unsuspected aerobic respiratory ability independent of whole mitochondria. This may be relevant for their ability to rescue cell bioenergetics. The differential oxidative metabolism of preterm vs. term exosomes sheds new light on the preterm newborn’s clinical vulnerability. A reduced ability to repair damaged tissue and an increased capability to cope with anoxic environment for preterm infants can be envisaged. (Less)
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Contribution to journal
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published
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keywords
ATP synthesis, oxidative phosphorylation, umbilical cord, electron transport chain, energy metabolism
in
FASEB Journal
publisher
The Federation of American Societies for Experimental Biology
external identifiers
  • scopus:84963894602
  • wos:000372629100005
ISSN
1530-6860
DOI
10.1096/fj.15-279679
language
English
LU publication?
no
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d50748cc-39f5-4008-9215-5bf1f3afff7b (old id 8696653)
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http://www.ncbi.nlm.nih.gov/pubmed/26655706
date added to LUP
2016-02-17 09:49:10
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2017-11-12 03:41:19
@article{d50748cc-39f5-4008-9215-5bf1f3afff7b,
  abstract     = {Exosomes are secreted nanovesicles that are able to transfer RNA and proteins to target cells. The emerging role of mesenchymal stem cell (MSC) exosomes as promoters of aerobic ATP synthesis restoration in damaged cells, prompted us to assess whether they contain an extramitochondrial aerobic respiration capacity. Exosomes were isolated from culture medium of human MSC from umbilical cord of ≥37-wk-old newborns or between 28- to 30–wk-old newborns (i.e., term or preterm infants). Characterization of samples was conducted by cytofluorometry. Oxidative phosphorylation capacity was assessed by Western blot analysis, oximetry, and luminometric and fluorometric analyses. MSC exosomes express functional respiratory complexes I, IV, and V, consuming oxygen. ATP synthesis was only detectable in exosomes from term newborns, suggestive of a specific mechanism that is not completed at an early gestational age. Activities are outward facing and comparable to those detected in mitochondria isolated from term MSC. MSC exosomes display an unsuspected aerobic respiratory ability independent of whole mitochondria. This may be relevant for their ability to rescue cell bioenergetics. The differential oxidative metabolism of preterm vs. term exosomes sheds new light on the preterm newborn’s clinical vulnerability. A reduced ability to repair damaged tissue and an increased capability to cope with anoxic environment for preterm infants can be envisaged.},
  author       = {Panfoli, I. and Ravera, S. and Podesta, M. and Cossu, C. and Santucci, L. and Bartolucci, M. and Brusch, M. and Calzia, D. and Sabatini, F. and Bruschettini, Matteo and Ramenghi, L. A. and Romanski, O. and Marimpietri, D. and Pistoia, V. and Ghiggeri, G. and Frassoni, F. and Candiano, G.},
  issn         = {1530-6860},
  keyword      = {ATP synthesis,oxidative phosphorylation,umbilical cord,electron transport chain,energy metabolism},
  language     = {eng},
  publisher    = {The Federation of American Societies for Experimental Biology},
  series       = {FASEB Journal},
  title        = {Exosomes from human mesenchymal stem cells conduct aerobic metabolism in term and preterm newborn infants},
  url          = {http://dx.doi.org/10.1096/fj.15-279679},
  year         = {2016},
}