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Effects of metabolite binding to ribulosebisphosphate carboxylase on the activity of the Calvin photosynthesis cycle

Pettersson, Gösta LU and Ryde-Pettersson, Ulf LU (1988) In European Journal of Biochemistry 177(2). p.351-355
Abstract

The regulatory implications of the interaction of ribulosebisphosphate carboxylase with metabolites participating in the Calvin photosynthesis cycle has been examined by control analysis based on our recently described kinetic model for photosynthetic carbohydrate formation in the chloroplast of C3 plants. The results provide clear evidence that the Calvin cycle activity under conditions of light and CO2 saturation is insignificantly affected by the inhibition of ribulosebisphosphate carboxylase caused by metabolites such as 3‐phosphoglycerate, fructose 1,6‐bisphosphate, sedoheptulose 1,7‐bisphosphate, NADPH, and inorganic orthophosphate. Due to the exceptionally high stromal concentration of the carboxylase,... (More)

The regulatory implications of the interaction of ribulosebisphosphate carboxylase with metabolites participating in the Calvin photosynthesis cycle has been examined by control analysis based on our recently described kinetic model for photosynthetic carbohydrate formation in the chloroplast of C3 plants. The results provide clear evidence that the Calvin cycle activity under conditions of light and CO2 saturation is insignificantly affected by the inhibition of ribulosebisphosphate carboxylase caused by metabolites such as 3‐phosphoglycerate, fructose 1,6‐bisphosphate, sedoheptulose 1,7‐bisphosphate, NADPH, and inorganic orthophosphate. Due to the exceptionally high stromal concentration of the carboxylase, metabolite binding to the enzyme affects the Calvin cycle activity indirectly by reducing the pool of free orthophosphate and phosphorylated metabolites available for the cyclic reactions. This pool reduction corresponds typically to about 5 mM total phosphate and derives mainly from the binding of ribulose bisphosphate and orthophosphate. Substantial amounts of the metabolites interacting with ribulosebisphosphate carboxylase are present in an enzyme‐bound form. The bound form of the Calvin cycle intermediates sedoheptulose bisphosphate, fructose bisphosphate, and ribulose bisphosphate typically accounts for about 70, 80, and 90%, respectively, of the total stromal concentration of the intermediate. Copyright © 1988, Wiley Blackwell. All rights reserved

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
European Journal of Biochemistry
volume
177
issue
2
pages
5 pages
publisher
Wiley-Blackwell
external identifiers
  • scopus:0024119377
ISSN
0014-2956
DOI
10.1111/j.1432-1033.1988.tb14382.x
language
English
LU publication?
yes
id
86f96f83-61d1-4eb1-8da2-673f4c536582
date added to LUP
2017-02-04 11:24:41
date last changed
2017-03-06 17:02:32
@article{86f96f83-61d1-4eb1-8da2-673f4c536582,
  abstract     = {<p>The regulatory implications of the interaction of ribulosebisphosphate carboxylase with metabolites participating in the Calvin photosynthesis cycle has been examined by control analysis based on our recently described kinetic model for photosynthetic carbohydrate formation in the chloroplast of C<sub>3</sub> plants. The results provide clear evidence that the Calvin cycle activity under conditions of light and CO<sub>2</sub> saturation is insignificantly affected by the inhibition of ribulosebisphosphate carboxylase caused by metabolites such as 3‐phosphoglycerate, fructose 1,6‐bisphosphate, sedoheptulose 1,7‐bisphosphate, NADPH, and inorganic orthophosphate. Due to the exceptionally high stromal concentration of the carboxylase, metabolite binding to the enzyme affects the Calvin cycle activity indirectly by reducing the pool of free orthophosphate and phosphorylated metabolites available for the cyclic reactions. This pool reduction corresponds typically to about 5 mM total phosphate and derives mainly from the binding of ribulose bisphosphate and orthophosphate. Substantial amounts of the metabolites interacting with ribulosebisphosphate carboxylase are present in an enzyme‐bound form. The bound form of the Calvin cycle intermediates sedoheptulose bisphosphate, fructose bisphosphate, and ribulose bisphosphate typically accounts for about 70, 80, and 90%, respectively, of the total stromal concentration of the intermediate. Copyright © 1988, Wiley Blackwell. All rights reserved</p>},
  author       = {Pettersson, Gösta and Ryde-Pettersson, Ulf},
  issn         = {0014-2956},
  language     = {eng},
  number       = {2},
  pages        = {351--355},
  publisher    = {Wiley-Blackwell},
  series       = {European Journal of Biochemistry},
  title        = {Effects of metabolite binding to ribulosebisphosphate carboxylase on the activity of the Calvin photosynthesis cycle},
  url          = {http://dx.doi.org/10.1111/j.1432-1033.1988.tb14382.x},
  volume       = {177},
  year         = {1988},
}