Effects of metabolite binding to ribulosebisphosphate carboxylase on the activity of the Calvin photosynthesis cycle
Pettersson, Gösta LU and Ryde-Pettersson, Ulf LU
(1988)
In European Journal of Biochemistry
177(2).
p.351-355
- Abstract
The regulatory implications of the interaction of ribulosebisphosphate carboxylase with metabolites participating in the Calvin photosynthesis cycle has been examined by control analysis based on our recently described kinetic model for photosynthetic carbohydrate formation in the chloroplast of C3 plants. The results provide clear evidence that the Calvin cycle activity under conditions of light and CO2 saturation is insignificantly affected by the inhibition of ribulosebisphosphate carboxylase caused by metabolites such as 3‐phosphoglycerate, fructose 1,6‐bisphosphate, sedoheptulose 1,7‐bisphosphate, NADPH, and inorganic orthophosphate. Due to the exceptionally high stromal concentration of the carboxylase,... (More)
The regulatory implications of the interaction of ribulosebisphosphate carboxylase with metabolites participating in the Calvin photosynthesis cycle has been examined by control analysis based on our recently described kinetic model for photosynthetic carbohydrate formation in the chloroplast of C3 plants. The results provide clear evidence that the Calvin cycle activity under conditions of light and CO2 saturation is insignificantly affected by the inhibition of ribulosebisphosphate carboxylase caused by metabolites such as 3‐phosphoglycerate, fructose 1,6‐bisphosphate, sedoheptulose 1,7‐bisphosphate, NADPH, and inorganic orthophosphate. Due to the exceptionally high stromal concentration of the carboxylase, metabolite binding to the enzyme affects the Calvin cycle activity indirectly by reducing the pool of free orthophosphate and phosphorylated metabolites available for the cyclic reactions. This pool reduction corresponds typically to about 5 mM total phosphate and derives mainly from the binding of ribulose bisphosphate and orthophosphate. Substantial amounts of the metabolites interacting with ribulosebisphosphate carboxylase are present in an enzyme‐bound form. The bound form of the Calvin cycle intermediates sedoheptulose bisphosphate, fructose bisphosphate, and ribulose bisphosphate typically accounts for about 70, 80, and 90%, respectively, of the total stromal concentration of the intermediate.
(Less)
- author
- Pettersson, Gösta
LU
and Ryde-Pettersson, Ulf
LU
- organization
- publishing date
- 1988
- type
- Contribution to journal
- publication status
- published
- subject
- in
- European Journal of Biochemistry
- volume
- 177
- issue
- 2
- pages
- 5 pages
- publisher
- Wiley-Blackwell
- external identifiers
-
- pmid:3142774
- scopus:85047693172
- ISSN
- 0014-2956
- DOI
- 10.1111/j.1432-1033.1988.tb14382.x
- language
- English
- LU publication?
- yes
- id
- 86f96f83-61d1-4eb1-8da2-673f4c536582
- date added to LUP
- 2017-02-04 11:24:41
- date last changed
- 2023-05-08 04:01:27
@article{86f96f83-61d1-4eb1-8da2-673f4c536582,
abstract = {{<p>The regulatory implications of the interaction of ribulosebisphosphate carboxylase with metabolites participating in the Calvin photosynthesis cycle has been examined by control analysis based on our recently described kinetic model for photosynthetic carbohydrate formation in the chloroplast of C<sub>3</sub> plants. The results provide clear evidence that the Calvin cycle activity under conditions of light and CO<sub>2</sub> saturation is insignificantly affected by the inhibition of ribulosebisphosphate carboxylase caused by metabolites such as 3‐phosphoglycerate, fructose 1,6‐bisphosphate, sedoheptulose 1,7‐bisphosphate, NADPH, and inorganic orthophosphate. Due to the exceptionally high stromal concentration of the carboxylase, metabolite binding to the enzyme affects the Calvin cycle activity indirectly by reducing the pool of free orthophosphate and phosphorylated metabolites available for the cyclic reactions. This pool reduction corresponds typically to about 5 mM total phosphate and derives mainly from the binding of ribulose bisphosphate and orthophosphate. Substantial amounts of the metabolites interacting with ribulosebisphosphate carboxylase are present in an enzyme‐bound form. The bound form of the Calvin cycle intermediates sedoheptulose bisphosphate, fructose bisphosphate, and ribulose bisphosphate typically accounts for about 70, 80, and 90%, respectively, of the total stromal concentration of the intermediate.</p>}},
author = {{Pettersson, Gösta and Ryde-Pettersson, Ulf}},
issn = {{0014-2956}},
language = {{eng}},
number = {{2}},
pages = {{351--355}},
publisher = {{Wiley-Blackwell}},
series = {{European Journal of Biochemistry}},
title = {{Effects of metabolite binding to ribulosebisphosphate carboxylase on the activity of the Calvin photosynthesis cycle}},
url = {{https://lup.lub.lu.se/search/files/24720483/calvin3_rubpase.pdf}},
doi = {{10.1111/j.1432-1033.1988.tb14382.x}},
volume = {{177}},
year = {{1988}},
}