Identification and characterization of secretagogin promoter activity
(2006) In Scandinavian Journal of Immunology 64(6). p.639-645- Abstract
- Secretagogin is a newly identified calcium-binding protein selectively expressed in neuroendocrine tissue and pancreatic beta-cells. The function of secretagogin is unknown, but it has been suggested in beta-cells to influence calcium-influx, insulin secretion and proliferation, and has been observed downregulated in diabetes-prone BB rat islets exposed to cytokines. In the present study, we identified and characterized promoter activity of a human 1498 bp sequence upstream the transcription start site. The promoter sequence showed subtle but significant regulation by glucose within the normo-physiological range. Glucose also led to changes in expression of secretagogin protein in INS-1e cells, but not in primary cells from... (More)
- Secretagogin is a newly identified calcium-binding protein selectively expressed in neuroendocrine tissue and pancreatic beta-cells. The function of secretagogin is unknown, but it has been suggested in beta-cells to influence calcium-influx, insulin secretion and proliferation, and has been observed downregulated in diabetes-prone BB rat islets exposed to cytokines. In the present study, we identified and characterized promoter activity of a human 1498 bp sequence upstream the transcription start site. The promoter sequence showed subtle but significant regulation by glucose within the normo-physiological range. Glucose also led to changes in expression of secretagogin protein in INS-1e cells, but not in primary cells from non-diabetes-prone Wistar Furth rats. No effects of cytokines neither on promoter activity nor protein expression were observed. The promoter region was furthermore screened by direct sequencing, and 11 polymorphisms were identified. Genotyping in a large homogenous Type 1 diabetes (T1D) family collection did not reveal association with T1D. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/377335
- author
- Skovhus, K. V. ; Bergholdt, R ; Erichsen, C ; Sparre, T. ; Nerup, Jörn LU ; Karlsen, A. E. and Pociot, F.
- organization
- publishing date
- 2006
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Scandinavian Journal of Immunology
- volume
- 64
- issue
- 6
- pages
- 639 - 645
- publisher
- Wiley-Blackwell
- external identifiers
-
- wos:000241776300008
- scopus:33750700761
- ISSN
- 1365-3083
- DOI
- 10.1111/j.1365-3083.2006.01854.x
- language
- English
- LU publication?
- yes
- id
- 870595f7-f00a-4a1b-a50a-16e14ce941aa (old id 377335)
- date added to LUP
- 2016-04-01 17:05:41
- date last changed
- 2022-04-23 02:37:20
@article{870595f7-f00a-4a1b-a50a-16e14ce941aa,
abstract = {{Secretagogin is a newly identified calcium-binding protein selectively expressed in neuroendocrine tissue and pancreatic beta-cells. The function of secretagogin is unknown, but it has been suggested in beta-cells to influence calcium-influx, insulin secretion and proliferation, and has been observed downregulated in diabetes-prone BB rat islets exposed to cytokines. In the present study, we identified and characterized promoter activity of a human 1498 bp sequence upstream the transcription start site. The promoter sequence showed subtle but significant regulation by glucose within the normo-physiological range. Glucose also led to changes in expression of secretagogin protein in INS-1e cells, but not in primary cells from non-diabetes-prone Wistar Furth rats. No effects of cytokines neither on promoter activity nor protein expression were observed. The promoter region was furthermore screened by direct sequencing, and 11 polymorphisms were identified. Genotyping in a large homogenous Type 1 diabetes (T1D) family collection did not reveal association with T1D.}},
author = {{Skovhus, K. V. and Bergholdt, R and Erichsen, C and Sparre, T. and Nerup, Jörn and Karlsen, A. E. and Pociot, F.}},
issn = {{1365-3083}},
language = {{eng}},
number = {{6}},
pages = {{639--645}},
publisher = {{Wiley-Blackwell}},
series = {{Scandinavian Journal of Immunology}},
title = {{Identification and characterization of secretagogin promoter activity}},
url = {{http://dx.doi.org/10.1111/j.1365-3083.2006.01854.x}},
doi = {{10.1111/j.1365-3083.2006.01854.x}},
volume = {{64}},
year = {{2006}},
}