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Amperometric determination of acetate with a tri-enzyme based sensor

Mieliauskiene, R; Nistor, Mihaela LU ; Laurinavicius, V and Csöregi, Elisabeth LU (2006) In Sensors and Actuators B: Chemical 113(2). p.671-676
Abstract
The present work describes a flow injection analysis system incorporating amperometric biosensors for acetate monitoring. Tri-enzyme-modified electrodes were fabricated by immobilizing acetate kinase (AK), pyruvate kinase (PK) and lactate dehydrogenase (LDH) into a poly(ethyleneglycole) diglycidyl ether (PEGDGE) film also containing Brilliant Cresyl Blue (BCB) as an electrochemical mediator. The concentration of acetate could be determined from the decrease in the amperometric current at +50 mV versus Ag/AgCl. Parameters, such as composition of the biosensing film, operational conditions (e.g., applied potential, flow rate) were optimized. The optimized biosensor showed a linear response to acetate in the range of 0.2-8 mM with a detection... (More)
The present work describes a flow injection analysis system incorporating amperometric biosensors for acetate monitoring. Tri-enzyme-modified electrodes were fabricated by immobilizing acetate kinase (AK), pyruvate kinase (PK) and lactate dehydrogenase (LDH) into a poly(ethyleneglycole) diglycidyl ether (PEGDGE) film also containing Brilliant Cresyl Blue (BCB) as an electrochemical mediator. The concentration of acetate could be determined from the decrease in the amperometric current at +50 mV versus Ag/AgCl. Parameters, such as composition of the biosensing film, operational conditions (e.g., applied potential, flow rate) were optimized. The optimized biosensor showed a linear response to acetate in the range of 0.2-8 mM with a detection limit of 0.13 mM (calculated as 3 SIN). The acetate content of different samples (wine and vinegar) could be reliably quantified with the described biosensor. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
lactate, pyruvate kinase, acetate biosensor, acetate kinase, dehydrogenase
in
Sensors and Actuators B: Chemical
volume
113
issue
2
pages
671 - 676
publisher
Elsevier
external identifiers
  • wos:000235593200014
  • scopus:31844439649
ISSN
0925-4005
DOI
10.1016/j.snb.2005.07.016
language
English
LU publication?
yes
id
871d7d99-85a1-4fc7-bcf1-46e4d281a825 (old id 417028)
date added to LUP
2007-10-10 14:07:21
date last changed
2019-02-20 08:13:53
@article{871d7d99-85a1-4fc7-bcf1-46e4d281a825,
  abstract     = {The present work describes a flow injection analysis system incorporating amperometric biosensors for acetate monitoring. Tri-enzyme-modified electrodes were fabricated by immobilizing acetate kinase (AK), pyruvate kinase (PK) and lactate dehydrogenase (LDH) into a poly(ethyleneglycole) diglycidyl ether (PEGDGE) film also containing Brilliant Cresyl Blue (BCB) as an electrochemical mediator. The concentration of acetate could be determined from the decrease in the amperometric current at +50 mV versus Ag/AgCl. Parameters, such as composition of the biosensing film, operational conditions (e.g., applied potential, flow rate) were optimized. The optimized biosensor showed a linear response to acetate in the range of 0.2-8 mM with a detection limit of 0.13 mM (calculated as 3 SIN). The acetate content of different samples (wine and vinegar) could be reliably quantified with the described biosensor.},
  author       = {Mieliauskiene, R and Nistor, Mihaela and Laurinavicius, V and Csöregi, Elisabeth},
  issn         = {0925-4005},
  keyword      = {lactate,pyruvate kinase,acetate biosensor,acetate kinase,dehydrogenase},
  language     = {eng},
  number       = {2},
  pages        = {671--676},
  publisher    = {Elsevier},
  series       = {Sensors and Actuators B: Chemical},
  title        = {Amperometric determination of acetate with a tri-enzyme based sensor},
  url          = {http://dx.doi.org/10.1016/j.snb.2005.07.016},
  volume       = {113},
  year         = {2006},
}