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Acute hypothalamo-pituitary-adrenal axis response to LPS-induced endotoxemia: expression pattern of kinin type B1 and B2 receptors

Qadri, Fatimunnisa; Rimmele, Florian; Mallis, Lisa; Haeuser, Walter; Dendorfer, Andreas; Joehren, Olaf; Dominiak, Peter; Leeb-Lundberg, Fredrik LU and Bader, Michael (2016) In Biological Chemistry 397(2). p.97-109
Abstract
Bradykinin (BK) and des-Arg(9)-BK are pro-inflammatory mediators acting via B2 (B2R) and B1 (B1R) receptors, respectively. We investigated the role of B2R and B1R in lipopolysaccharide (LPS)-induced hypothalamopituitary-adrenal (HPA) axis activation in SD rats. LPS given intraperitoneally (ip) up-regulated B1R mRNA in the hypothalamus, both B1R and B2R were up-regulated in pituitary and adrenal glands. Receptor localization was performed using immunofluorescence staining. B1R was localized in the endothelial cells, nucleus supraopticus (SON), adenohypophysis and adrenal cortex. B2R was localized nucleus paraventricularis (PVN) and SON, pituitary and adrenal medulla. Blockade of B1R prior to LPS further increased ACTH release and blockade... (More)
Bradykinin (BK) and des-Arg(9)-BK are pro-inflammatory mediators acting via B2 (B2R) and B1 (B1R) receptors, respectively. We investigated the role of B2R and B1R in lipopolysaccharide (LPS)-induced hypothalamopituitary-adrenal (HPA) axis activation in SD rats. LPS given intraperitoneally (ip) up-regulated B1R mRNA in the hypothalamus, both B1R and B2R were up-regulated in pituitary and adrenal glands. Receptor localization was performed using immunofluorescence staining. B1R was localized in the endothelial cells, nucleus supraopticus (SON), adenohypophysis and adrenal cortex. B2R was localized nucleus paraventricularis (PVN) and SON, pituitary and adrenal medulla. Blockade of B1R prior to LPS further increased ACTH release and blockade of B1R 1 h after LPS decreased its release. In addition, we evaluated if blockade of central kinin receptors influence the LPS-induced stimulation of hypothalamic neurons. Blockade of both B1R and B2R reduced the LPS-induced c-Fos immunoreactivity in the hypothalamus. Our data demonstrate that a single injection of LPS induced a differential expression pattern of kinin B1R and B2R in the HPA axis. The tissue specific cellular localization of these receptors indicates that they may play a crucial role in the maintenance of body homeostasis during endotoxemia. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
B1R, B2R, c-Fos, HPA-axis, receptor localization, sepsis
in
Biological Chemistry
volume
397
issue
2
pages
97 - 109
publisher
De Gruyter
external identifiers
  • wos:000368268300001
  • scopus:84955454485
ISSN
1437-4315
DOI
10.1515/hsz-2015-0206
language
English
LU publication?
yes
id
c2431143-e747-42b3-ac1d-54bc4909b109 (old id 8739106)
date added to LUP
2016-02-23 11:08:22
date last changed
2017-10-22 03:08:22
@article{c2431143-e747-42b3-ac1d-54bc4909b109,
  abstract     = {Bradykinin (BK) and des-Arg(9)-BK are pro-inflammatory mediators acting via B2 (B2R) and B1 (B1R) receptors, respectively. We investigated the role of B2R and B1R in lipopolysaccharide (LPS)-induced hypothalamopituitary-adrenal (HPA) axis activation in SD rats. LPS given intraperitoneally (ip) up-regulated B1R mRNA in the hypothalamus, both B1R and B2R were up-regulated in pituitary and adrenal glands. Receptor localization was performed using immunofluorescence staining. B1R was localized in the endothelial cells, nucleus supraopticus (SON), adenohypophysis and adrenal cortex. B2R was localized nucleus paraventricularis (PVN) and SON, pituitary and adrenal medulla. Blockade of B1R prior to LPS further increased ACTH release and blockade of B1R 1 h after LPS decreased its release. In addition, we evaluated if blockade of central kinin receptors influence the LPS-induced stimulation of hypothalamic neurons. Blockade of both B1R and B2R reduced the LPS-induced c-Fos immunoreactivity in the hypothalamus. Our data demonstrate that a single injection of LPS induced a differential expression pattern of kinin B1R and B2R in the HPA axis. The tissue specific cellular localization of these receptors indicates that they may play a crucial role in the maintenance of body homeostasis during endotoxemia.},
  author       = {Qadri, Fatimunnisa and Rimmele, Florian and Mallis, Lisa and Haeuser, Walter and Dendorfer, Andreas and Joehren, Olaf and Dominiak, Peter and Leeb-Lundberg, Fredrik and Bader, Michael},
  issn         = {1437-4315},
  keyword      = {B1R,B2R,c-Fos,HPA-axis,receptor localization,sepsis},
  language     = {eng},
  number       = {2},
  pages        = {97--109},
  publisher    = {De Gruyter},
  series       = {Biological Chemistry},
  title        = {Acute hypothalamo-pituitary-adrenal axis response to LPS-induced endotoxemia: expression pattern of kinin type B1 and B2 receptors},
  url          = {http://dx.doi.org/10.1515/hsz-2015-0206},
  volume       = {397},
  year         = {2016},
}