Advanced

Dendritic cell subpopulations in nasopharyngeal cancer

Nilsson, Johan S. LU ; Abolhalaj, Milad LU ; Lundberg, Kristina LU ; Lindstedt, Malin LU and Greiff, Lennart LU (2019) In Oncology Letters 17(2). p.2557-2561
Abstract

Nasopharyngeal cancer (NPC) is associated with Epstein-Barr virus (EBV) and EBV antigen may be utilized for therapeutic purposes, including targeting of dendritic cells (DCs). Although DCs may be present in NPC, the information is limited and not up-to-date with current knowledge on DC subsets. In the present study, biopsies from untreated NPC were obtained and subjected to multicolor flow-cytometry focusing on DC subtype markers: CD123 for plasmacytoid DCs (pDCs); and CD1c and CD141 for myeloid DCs (mDCs). Furthermore, subset-specific expression of the C-lectin receptor (CLR) CD207 (also termed langerin) was assessed. pDCs and mDCs were detected in the NPC lesions, contributing to a frequency mean average of 0.78% of CD45+... (More)

Nasopharyngeal cancer (NPC) is associated with Epstein-Barr virus (EBV) and EBV antigen may be utilized for therapeutic purposes, including targeting of dendritic cells (DCs). Although DCs may be present in NPC, the information is limited and not up-to-date with current knowledge on DC subsets. In the present study, biopsies from untreated NPC were obtained and subjected to multicolor flow-cytometry focusing on DC subtype markers: CD123 for plasmacytoid DCs (pDCs); and CD1c and CD141 for myeloid DCs (mDCs). Furthermore, subset-specific expression of the C-lectin receptor (CLR) CD207 (also termed langerin) was assessed. pDCs and mDCs were detected in the NPC lesions, contributing to a frequency mean average of 0.78% of CD45+ leukocytes in situ. Different subpopulations, previously not described in NPC, were observed, including: CD123+ pDCs; CD1c+ mDCs; CD141+ mDCs; and CD1c-CD141- mDCs. A high frequency of CD1c+ mDCs expressing CD207 was observed, compared with other subsets. In conclusion, different DC subsets are present in NPC lesions. The CLR CD207, a selective endocytic marker on CD1c+ mDCs, may be targeted for therapeutic purposes to facilitate cross-presentation of antigens and aid cell-mediated antitumor effects.

(Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Antigen presentation, C-lectin receptor, Langerin, Pattern recognition receptor
in
Oncology Letters
volume
17
issue
2
pages
5 pages
publisher
Spandidos Publications
external identifiers
  • scopus:85059642872
ISSN
1792-1074
DOI
10.3892/ol.2018.9835
language
English
LU publication?
yes
id
874c3a45-fc01-40b1-a138-dd27ccc8c420
date added to LUP
2019-01-17 13:09:03
date last changed
2019-02-20 11:43:32
@article{874c3a45-fc01-40b1-a138-dd27ccc8c420,
  abstract     = {<p>Nasopharyngeal cancer (NPC) is associated with Epstein-Barr virus (EBV) and EBV antigen may be utilized for therapeutic purposes, including targeting of dendritic cells (DCs). Although DCs may be present in NPC, the information is limited and not up-to-date with current knowledge on DC subsets. In the present study, biopsies from untreated NPC were obtained and subjected to multicolor flow-cytometry focusing on DC subtype markers: CD123 for plasmacytoid DCs (pDCs); and CD1c and CD141 for myeloid DCs (mDCs). Furthermore, subset-specific expression of the C-lectin receptor (CLR) CD207 (also termed langerin) was assessed. pDCs and mDCs were detected in the NPC lesions, contributing to a frequency mean average of 0.78% of CD45<sup>+</sup> leukocytes in situ. Different subpopulations, previously not described in NPC, were observed, including: CD123<sup>+</sup> pDCs; CD1c<sup>+</sup> mDCs; CD141<sup>+</sup> mDCs; and CD1c<sup>-</sup>CD141<sup>-</sup> mDCs. A high frequency of CD1c<sup>+</sup> mDCs expressing CD207 was observed, compared with other subsets. In conclusion, different DC subsets are present in NPC lesions. The CLR CD207, a selective endocytic marker on CD1c<sup>+</sup> mDCs, may be targeted for therapeutic purposes to facilitate cross-presentation of antigens and aid cell-mediated antitumor effects.</p>},
  author       = {Nilsson, Johan S. and Abolhalaj, Milad and Lundberg, Kristina and Lindstedt, Malin and Greiff, Lennart},
  issn         = {1792-1074},
  keyword      = {Antigen presentation,C-lectin receptor,Langerin,Pattern recognition receptor},
  language     = {eng},
  number       = {2},
  pages        = {2557--2561},
  publisher    = {Spandidos Publications},
  series       = {Oncology Letters},
  title        = {Dendritic cell subpopulations in nasopharyngeal cancer},
  url          = {http://dx.doi.org/10.3892/ol.2018.9835},
  volume       = {17},
  year         = {2019},
}