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Microcontact imprinting based surface plasmon resonance (SPR) biosensor for real-time and ultrasensitive detection of prostate specific antigen (PSA) from clinical samples

Erturk, Gizem; Ozen, Haluk; Tumer, M. Askin; Mattiasson, Bo LU and Denizli, Adil (2016) In Sensors and Actuators B: Chemical 224. p.823-832
Abstract
Prostate specific antigen (PSA) is an important biomarker for diagnosis and prognosis of prostate cancer. Herein, microcontact PSA-imprinted surface plasmon resonance (SPR) sensor chip was developed for sensitive, real-time detection of PSA. The imprinted chip was prepared in the presence of methacrylic acid (MAA) as functional monomer and ethylene glycol dimethacrylate (EGDMA) as cross-linker via UV polymerization using microcontact imprinting technique. PSA imprinted SPR sensor chip was characterized by atomic force microscope (AFM), scanning electron microscope (SEM), ellipsometry, dispersive Raman and Fourier transform infrared spectroscopy (FT-IR). Under optimal conditions, PSA detection was performed with standard PSA solutions in... (More)
Prostate specific antigen (PSA) is an important biomarker for diagnosis and prognosis of prostate cancer. Herein, microcontact PSA-imprinted surface plasmon resonance (SPR) sensor chip was developed for sensitive, real-time detection of PSA. The imprinted chip was prepared in the presence of methacrylic acid (MAA) as functional monomer and ethylene glycol dimethacrylate (EGDMA) as cross-linker via UV polymerization using microcontact imprinting technique. PSA imprinted SPR sensor chip was characterized by atomic force microscope (AFM), scanning electron microscope (SEM), ellipsometry, dispersive Raman and Fourier transform infrared spectroscopy (FT-IR). Under optimal conditions, PSA detection was performed with standard PSA solutions in the concentration range of 0.1-50 ng mL(-1) with a detection limit (LOD) of approximately 91 pg mL(-1) (18 x 10(-14) M). Selectivity studies were performed against human serum albumin (HSA) and lysozyme (Lyz) as the competitive agents. The developed system was evaluated for analysis of 10 clinical serum samples and showed approximately 98% agreement between the results obtained by commercial enzyme-linked immunosorbent assay (ELISA) method without significant differences at the 0.05 significance level (p = 0.751, p >0.05). (C) 2015 Elsevier B.V. All rights reserved. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Prostate specific antigen, Surface plasmon resonance, Microcontact, imprinting, Enzyme-linked immunosorbent assay
in
Sensors and Actuators B: Chemical
volume
224
pages
823 - 832
publisher
Elsevier
external identifiers
  • wos:000366459500101
  • scopus:84947273664
ISSN
0925-4005
DOI
10.1016/j.snb.2015.10.093
language
English
LU publication?
yes
id
b34678d6-1851-4e50-acb3-176140eaa1cb (old id 8754570)
date added to LUP
2016-02-23 07:42:05
date last changed
2017-09-24 04:03:42
@article{b34678d6-1851-4e50-acb3-176140eaa1cb,
  abstract     = {Prostate specific antigen (PSA) is an important biomarker for diagnosis and prognosis of prostate cancer. Herein, microcontact PSA-imprinted surface plasmon resonance (SPR) sensor chip was developed for sensitive, real-time detection of PSA. The imprinted chip was prepared in the presence of methacrylic acid (MAA) as functional monomer and ethylene glycol dimethacrylate (EGDMA) as cross-linker via UV polymerization using microcontact imprinting technique. PSA imprinted SPR sensor chip was characterized by atomic force microscope (AFM), scanning electron microscope (SEM), ellipsometry, dispersive Raman and Fourier transform infrared spectroscopy (FT-IR). Under optimal conditions, PSA detection was performed with standard PSA solutions in the concentration range of 0.1-50 ng mL(-1) with a detection limit (LOD) of approximately 91 pg mL(-1) (18 x 10(-14) M). Selectivity studies were performed against human serum albumin (HSA) and lysozyme (Lyz) as the competitive agents. The developed system was evaluated for analysis of 10 clinical serum samples and showed approximately 98% agreement between the results obtained by commercial enzyme-linked immunosorbent assay (ELISA) method without significant differences at the 0.05 significance level (p = 0.751, p >0.05). (C) 2015 Elsevier B.V. All rights reserved.},
  author       = {Erturk, Gizem and Ozen, Haluk and Tumer, M. Askin and Mattiasson, Bo and Denizli, Adil},
  issn         = {0925-4005},
  keyword      = {Prostate specific antigen,Surface plasmon resonance,Microcontact,imprinting,Enzyme-linked immunosorbent assay},
  language     = {eng},
  pages        = {823--832},
  publisher    = {Elsevier},
  series       = {Sensors and Actuators B: Chemical},
  title        = {Microcontact imprinting based surface plasmon resonance (SPR) biosensor for real-time and ultrasensitive detection of prostate specific antigen (PSA) from clinical samples},
  url          = {http://dx.doi.org/10.1016/j.snb.2015.10.093},
  volume       = {224},
  year         = {2016},
}