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Detection of cancerous kidney tissue by means of SERS spectroscopy of extracellular fluid

Velicka, M.; Pucetaite, M. LU ; Urboniene, V.; Ceponkus, Justinas; Jankevicius, F. and Sablinskas, Valdas (2017) In Journal of Raman Spectroscopy 48(12). p.1744-1754
Abstract

We present a novel approach for detection of cancerous kidney tissue areas by measuring surface enhanced Raman scattering (SERS) spectra of extracellular fluid taken from kidney tissue. The method is based on spectral analysis of the cancerous and normal tissue areas in order to find the specific spectral markers. The samples were prepared by sliding the kidney tissue over a substrate—calcium fluoride optical window. For producing the SERS signal, the dried extracellular fluid film was covered by silver nanoparticle colloidal solution. In order to suppress fluorescence background, the measurements of the dried samples were performed in the NIR spectral region with the Raman excitation wavelength of 1,064 nm. The most significant... (More)

We present a novel approach for detection of cancerous kidney tissue areas by measuring surface enhanced Raman scattering (SERS) spectra of extracellular fluid taken from kidney tissue. The method is based on spectral analysis of the cancerous and normal tissue areas in order to find the specific spectral markers. The samples were prepared by sliding the kidney tissue over a substrate—calcium fluoride optical window. For producing the SERS signal, the dried extracellular fluid film was covered by silver nanoparticle colloidal solution. In order to suppress fluorescence background, the measurements of the dried samples were performed in the NIR spectral region with the Raman excitation wavelength of 1,064 nm. The most significant spectral differences—spectral markers—were found in the wavenumber region between 400 and 1,800 cm−1, where spectral bands related to various vibrations of carbohydrates, amino acids, and nucleic acids are located. Spectral markers in the SERS spectra are different from those in IR absorption spectra. The SERS spectroscopic method has a potential to be used directly during the surgery.

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author
publishing date
type
Contribution to journal
publication status
published
keywords
extracellular fluid, kidney tumor, SERS spectroscopy
in
Journal of Raman Spectroscopy
volume
48
issue
12
pages
1744 - 1754
publisher
John Wiley & Sons
external identifiers
  • scopus:85038400297
ISSN
0377-0486
DOI
10.1002/jrs.5232
language
English
LU publication?
no
id
875c87f7-6d48-47b7-9a88-bb260f375604
date added to LUP
2019-03-18 11:50:01
date last changed
2019-08-25 05:12:28
@article{875c87f7-6d48-47b7-9a88-bb260f375604,
  abstract     = {<p>We present a novel approach for detection of cancerous kidney tissue areas by measuring surface enhanced Raman scattering (SERS) spectra of extracellular fluid taken from kidney tissue. The method is based on spectral analysis of the cancerous and normal tissue areas in order to find the specific spectral markers. The samples were prepared by sliding the kidney tissue over a substrate—calcium fluoride optical window. For producing the SERS signal, the dried extracellular fluid film was covered by silver nanoparticle colloidal solution. In order to suppress fluorescence background, the measurements of the dried samples were performed in the NIR spectral region with the Raman excitation wavelength of 1,064 nm. The most significant spectral differences—spectral markers—were found in the wavenumber region between 400 and 1,800 cm<sup>−1</sup>, where spectral bands related to various vibrations of carbohydrates, amino acids, and nucleic acids are located. Spectral markers in the SERS spectra are different from those in IR absorption spectra. The SERS spectroscopic method has a potential to be used directly during the surgery.</p>},
  author       = {Velicka, M. and Pucetaite, M. and Urboniene, V. and Ceponkus, Justinas and Jankevicius, F. and Sablinskas, Valdas},
  issn         = {0377-0486},
  keyword      = {extracellular fluid,kidney tumor,SERS spectroscopy},
  language     = {eng},
  month        = {12},
  number       = {12},
  pages        = {1744--1754},
  publisher    = {John Wiley & Sons},
  series       = {Journal of Raman Spectroscopy},
  title        = {Detection of cancerous kidney tissue by means of SERS spectroscopy of extracellular fluid},
  url          = {http://dx.doi.org/10.1002/jrs.5232},
  volume       = {48},
  year         = {2017},
}