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In vitro tumorigenic assay : colony forming assay for cancer stem cells

Rajendran, Vijayalakshmi and Jain, Mayur Vilas LU (2018) In Methods in Molecular Biology 1692. p.89-95
Abstract

Colony forming or clonogenic assay is an in vitro quantitative technique to examine the capability of a single cell to grow into a large colony through clonal expansion. Clonogenic activity is a sensitive indicator of undifferentiated cancer stem cells. Here, we described the colony forming ability of the isolated breast cancer stem cells from the total population of cancer cells using double-layered, soft agarose-based assay. This method demonstrates that cancer stem cells can survive and generate colony growth in an anchorage-independent culture model. The 0.005% crystal violet solution is used in this assay to visualize the generated colonies.

Please use this url to cite or link to this publication:
author
organization
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
keywords
Agarose, Cancer stem cell, Clonogenic assay, Colony growth, Crystal violet
in
Methods in Molecular Biology
volume
1692
pages
7 pages
publisher
Humana Press
external identifiers
  • scopus:85030751988
ISSN
10643745
DOI
language
English
LU publication?
yes
id
87707f6b-cec7-4f70-af0f-5ab7cd4bd2d3
date added to LUP
2017-10-18 07:19:14
date last changed
2018-05-29 10:11:18
@inbook{87707f6b-cec7-4f70-af0f-5ab7cd4bd2d3,
  abstract     = {<p>Colony forming or clonogenic assay is an in vitro quantitative technique to examine the capability of a single cell to grow into a large colony through clonal expansion. Clonogenic activity is a sensitive indicator of undifferentiated cancer stem cells. Here, we described the colony forming ability of the isolated breast cancer stem cells from the total population of cancer cells using double-layered, soft agarose-based assay. This method demonstrates that cancer stem cells can survive and generate colony growth in an anchorage-independent culture model. The 0.005% crystal violet solution is used in this assay to visualize the generated colonies.</p>},
  author       = {Rajendran, Vijayalakshmi and Jain, Mayur Vilas},
  issn         = {10643745},
  keyword      = {Agarose,Cancer stem cell,Clonogenic assay,Colony growth,Crystal violet},
  language     = {eng},
  pages        = {89--95},
  publisher    = {Humana Press},
  series       = {Methods in Molecular Biology},
  title        = {In vitro tumorigenic assay : colony forming assay for cancer stem cells},
  url          = {http://dx.doi.org/},
  volume       = {1692},
  year         = {2018},
}