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In vitro tumorigenic assay : colony forming assay for cancer stem cells

Rajendran, Vijayalakshmi and Jain, Mayur Vilas LU (2018) In Methods in Molecular Biology 1692. p.89-95
Abstract

Colony forming or clonogenic assay is an in vitro quantitative technique to examine the capability of a single cell to grow into a large colony through clonal expansion. Clonogenic activity is a sensitive indicator of undifferentiated cancer stem cells. Here, we described the colony forming ability of the isolated breast cancer stem cells from the total population of cancer cells using double-layered, soft agarose-based assay. This method demonstrates that cancer stem cells can survive and generate colony growth in an anchorage-independent culture model. The 0.005% crystal violet solution is used in this assay to visualize the generated colonies.

Please use this url to cite or link to this publication:
author
and
organization
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
keywords
Agarose, Cancer stem cell, Clonogenic assay, Colony growth, Crystal violet
host publication
Methods in Molecular Biology
series title
Methods in Molecular Biology
volume
1692
pages
7 pages
publisher
Humana Press
external identifiers
  • scopus:85030751988
  • pmid:28986889
ISSN
10643745
DOI
10.1007/978-1-4939-7401-6_8
language
English
LU publication?
yes
id
87707f6b-cec7-4f70-af0f-5ab7cd4bd2d3
date added to LUP
2017-10-18 07:19:14
date last changed
2021-10-06 03:56:15
@inbook{87707f6b-cec7-4f70-af0f-5ab7cd4bd2d3,
  abstract     = {<p>Colony forming or clonogenic assay is an in vitro quantitative technique to examine the capability of a single cell to grow into a large colony through clonal expansion. Clonogenic activity is a sensitive indicator of undifferentiated cancer stem cells. Here, we described the colony forming ability of the isolated breast cancer stem cells from the total population of cancer cells using double-layered, soft agarose-based assay. This method demonstrates that cancer stem cells can survive and generate colony growth in an anchorage-independent culture model. The 0.005% crystal violet solution is used in this assay to visualize the generated colonies.</p>},
  author       = {Rajendran, Vijayalakshmi and Jain, Mayur Vilas},
  booktitle    = {Methods in Molecular Biology},
  issn         = {10643745},
  language     = {eng},
  pages        = {89--95},
  publisher    = {Humana Press},
  series       = {Methods in Molecular Biology},
  title        = {In vitro tumorigenic assay : colony forming assay for cancer stem cells},
  url          = {http://dx.doi.org/10.1007/978-1-4939-7401-6_8},
  doi          = {10.1007/978-1-4939-7401-6_8},
  volume       = {1692},
  year         = {2018},
}