Cellulase cross-linked enzyme aggregates (CLEA) activities can be modulated and enhanced by precipitant selection
(2017) In Journal of Chemical Technology and Biotechnology 92(7). p.1645-1649- Abstract
BACKGROUND: Crosslinked enzyme aggregates (CLEA) technology is a rapid and versatile method to produce immobilized enzymes via precipitation and cross-linking. A direct relationship between CLEA final activity and process parameters is however yet to be clarified. To address the issue, we have used a factorial design to test the formation and optimization of CLEA made from technical grade cellulase (EC 3.2.1.4). Three types of precipitant (ammonium sulfate, polyethylene glycol, tert-butyl alcohol) were used at varied levels of cross-linker concentration, cross-linking time, and temperature. RESULTS: It was found that the CLEAs produced with tert-butyl alcohol were inactive, whereas the polyethylene glycol- and ammonium sulfate-CLEA,... (More)
BACKGROUND: Crosslinked enzyme aggregates (CLEA) technology is a rapid and versatile method to produce immobilized enzymes via precipitation and cross-linking. A direct relationship between CLEA final activity and process parameters is however yet to be clarified. To address the issue, we have used a factorial design to test the formation and optimization of CLEA made from technical grade cellulase (EC 3.2.1.4). Three types of precipitant (ammonium sulfate, polyethylene glycol, tert-butyl alcohol) were used at varied levels of cross-linker concentration, cross-linking time, and temperature. RESULTS: It was found that the CLEAs produced with tert-butyl alcohol were inactive, whereas the polyethylene glycol- and ammonium sulfate-CLEA, recovered 29 and 17% of the free enzyme activity, respectively. Testing for re-usability, we observed that the polyethylene glycol-CLEA maintained 40% of the initial activity after four cycles, in contrast the ammonium sulfate-CLEA only maintained 10% of its activity after one cycle. CONCLUSION: Our study highlights the importance of evaluating the effect of the precipitant on final CLEA activity rather than re-solubilized enzyme activity. It was demonstrated that polyethylene glycol, despite not being able to precipitate the enzymes as readily as ammonium sulfate, resulted in better performing CLEA.
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- author
- Perzon, Alixander ; Dicko, Cedric LU ; Çobanoğlu, Özgür ; Yükselen, Onur ; Eryilmaz, Jitka and Dey, Estera Szwajcer LU
- organization
- publishing date
- 2017-07-01
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- cellulases, cross-linked enzyme aggregates, factorial design, precipitation, recycling
- in
- Journal of Chemical Technology and Biotechnology
- volume
- 92
- issue
- 7
- pages
- 5 pages
- publisher
- Wiley-Blackwell
- external identifiers
-
- wos:000403025500020
- scopus:85020270504
- ISSN
- 0268-2575
- DOI
- 10.1002/jctb.5160
- language
- English
- LU publication?
- yes
- id
- 877c4548-b2e8-4055-801a-0d0323b8ff41
- date added to LUP
- 2017-06-26 15:40:53
- date last changed
- 2024-07-08 23:01:03
@article{877c4548-b2e8-4055-801a-0d0323b8ff41, abstract = {{<p>BACKGROUND: Crosslinked enzyme aggregates (CLEA) technology is a rapid and versatile method to produce immobilized enzymes via precipitation and cross-linking. A direct relationship between CLEA final activity and process parameters is however yet to be clarified. To address the issue, we have used a factorial design to test the formation and optimization of CLEA made from technical grade cellulase (EC 3.2.1.4). Three types of precipitant (ammonium sulfate, polyethylene glycol, tert-butyl alcohol) were used at varied levels of cross-linker concentration, cross-linking time, and temperature. RESULTS: It was found that the CLEAs produced with tert-butyl alcohol were inactive, whereas the polyethylene glycol- and ammonium sulfate-CLEA, recovered 29 and 17% of the free enzyme activity, respectively. Testing for re-usability, we observed that the polyethylene glycol-CLEA maintained 40% of the initial activity after four cycles, in contrast the ammonium sulfate-CLEA only maintained 10% of its activity after one cycle. CONCLUSION: Our study highlights the importance of evaluating the effect of the precipitant on final CLEA activity rather than re-solubilized enzyme activity. It was demonstrated that polyethylene glycol, despite not being able to precipitate the enzymes as readily as ammonium sulfate, resulted in better performing CLEA.</p>}}, author = {{Perzon, Alixander and Dicko, Cedric and Çobanoğlu, Özgür and Yükselen, Onur and Eryilmaz, Jitka and Dey, Estera Szwajcer}}, issn = {{0268-2575}}, keywords = {{cellulases; cross-linked enzyme aggregates; factorial design; precipitation; recycling}}, language = {{eng}}, month = {{07}}, number = {{7}}, pages = {{1645--1649}}, publisher = {{Wiley-Blackwell}}, series = {{Journal of Chemical Technology and Biotechnology}}, title = {{Cellulase cross-linked enzyme aggregates (CLEA) activities can be modulated and enhanced by precipitant selection}}, url = {{http://dx.doi.org/10.1002/jctb.5160}}, doi = {{10.1002/jctb.5160}}, volume = {{92}}, year = {{2017}}, }