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Immune responses elicited by apoB-100-derived peptides in mice

Pierides, Chrysoulla; Bermudez-Fajardo, Alexandra; Nordin Fredrikson, Gunilla LU ; Nilsson, Jan LU and Oviedo-Orta, Ernesto (2013) In Immunologic Research 56(1). p.96-108
Abstract
Peptides derived from apolipoprotein B (apoB)-100 have been previously used in vaccine preparations to treat atherosclerosis. Such vaccines have been shown to reduce atherosclerotic plaque development by 50 % in experimental animals, and this effect is associated with induction of T helper (Th)2 immune responses. In this study we immunised apolipoprotein E-deficient (apoE(-/-)) mice with apoB-100-derived peptides P2, P45 and P210. Animals received BSA-conjugated peptides or peptide-loaded bone marrow-derived dendritic cells (DCs). We used enzyme-linked immunosorbent assays to assess the synthesis of anti-peptide-specific IgG1 and IgG2a as well as the levels of interleukin (IL-)10 and interferon gamma (IFN-gamma) in plasma of immunised... (More)
Peptides derived from apolipoprotein B (apoB)-100 have been previously used in vaccine preparations to treat atherosclerosis. Such vaccines have been shown to reduce atherosclerotic plaque development by 50 % in experimental animals, and this effect is associated with induction of T helper (Th)2 immune responses. In this study we immunised apolipoprotein E-deficient (apoE(-/-)) mice with apoB-100-derived peptides P2, P45 and P210. Animals received BSA-conjugated peptides or peptide-loaded bone marrow-derived dendritic cells (DCs). We used enzyme-linked immunosorbent assays to assess the synthesis of anti-peptide-specific IgG1 and IgG2a as well as the levels of interleukin (IL-)10 and interferon gamma (IFN-gamma) in plasma of immunised animals. We also measured the effect of immunisation on the number of spleen-derived CD4(+) and CD8(+) regulatory T cells (Tregs) in these animals. Peptide and peptide-loaded DC immunisation significantly increased the levels of peptide-specific immunoglobulins and the number of Tregs in apoE(-/-) mice. This was accompanied by a significant increase in the secretion of IL-10 with no effect on IFN-gamma levels. The results also show that the peptides can modulate the homing properties of DCs. Altogether, this study provides novel evidence for the immune mechanisms excerpted by apoB-100-derived peptides and their effect on Tregs and DCs relevant to their use in vaccine preparations. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Peptides, Dendritic cells, Regulatory T cells, Lymphocyte, Immune, response
in
Immunologic Research
volume
56
issue
1
pages
96 - 108
publisher
Humana Press
external identifiers
  • wos:000317849100009
  • scopus:84876417974
ISSN
0257-277X
DOI
10.1007/s12026-013-8383-1
language
English
LU publication?
yes
id
87a4a8e7-1844-4c03-a2b1-fade27806efd (old id 3843449)
date added to LUP
2013-06-20 13:38:40
date last changed
2019-02-20 05:10:07
@article{87a4a8e7-1844-4c03-a2b1-fade27806efd,
  abstract     = {Peptides derived from apolipoprotein B (apoB)-100 have been previously used in vaccine preparations to treat atherosclerosis. Such vaccines have been shown to reduce atherosclerotic plaque development by 50 % in experimental animals, and this effect is associated with induction of T helper (Th)2 immune responses. In this study we immunised apolipoprotein E-deficient (apoE(-/-)) mice with apoB-100-derived peptides P2, P45 and P210. Animals received BSA-conjugated peptides or peptide-loaded bone marrow-derived dendritic cells (DCs). We used enzyme-linked immunosorbent assays to assess the synthesis of anti-peptide-specific IgG1 and IgG2a as well as the levels of interleukin (IL-)10 and interferon gamma (IFN-gamma) in plasma of immunised animals. We also measured the effect of immunisation on the number of spleen-derived CD4(+) and CD8(+) regulatory T cells (Tregs) in these animals. Peptide and peptide-loaded DC immunisation significantly increased the levels of peptide-specific immunoglobulins and the number of Tregs in apoE(-/-) mice. This was accompanied by a significant increase in the secretion of IL-10 with no effect on IFN-gamma levels. The results also show that the peptides can modulate the homing properties of DCs. Altogether, this study provides novel evidence for the immune mechanisms excerpted by apoB-100-derived peptides and their effect on Tregs and DCs relevant to their use in vaccine preparations.},
  author       = {Pierides, Chrysoulla and Bermudez-Fajardo, Alexandra and Nordin Fredrikson, Gunilla and Nilsson, Jan and Oviedo-Orta, Ernesto},
  issn         = {0257-277X},
  keyword      = {Peptides,Dendritic cells,Regulatory T cells,Lymphocyte,Immune,response},
  language     = {eng},
  number       = {1},
  pages        = {96--108},
  publisher    = {Humana Press},
  series       = {Immunologic Research},
  title        = {Immune responses elicited by apoB-100-derived peptides in mice},
  url          = {http://dx.doi.org/10.1007/s12026-013-8383-1},
  volume       = {56},
  year         = {2013},
}