Immune responses elicited by apoB-100-derived peptides in mice
(2013) In Immunologic Research 56(1). p.96-108- Abstract
- Peptides derived from apolipoprotein B (apoB)-100 have been previously used in vaccine preparations to treat atherosclerosis. Such vaccines have been shown to reduce atherosclerotic plaque development by 50 % in experimental animals, and this effect is associated with induction of T helper (Th)2 immune responses. In this study we immunised apolipoprotein E-deficient (apoE(-/-)) mice with apoB-100-derived peptides P2, P45 and P210. Animals received BSA-conjugated peptides or peptide-loaded bone marrow-derived dendritic cells (DCs). We used enzyme-linked immunosorbent assays to assess the synthesis of anti-peptide-specific IgG1 and IgG2a as well as the levels of interleukin (IL-)10 and interferon gamma (IFN-gamma) in plasma of immunised... (More)
- Peptides derived from apolipoprotein B (apoB)-100 have been previously used in vaccine preparations to treat atherosclerosis. Such vaccines have been shown to reduce atherosclerotic plaque development by 50 % in experimental animals, and this effect is associated with induction of T helper (Th)2 immune responses. In this study we immunised apolipoprotein E-deficient (apoE(-/-)) mice with apoB-100-derived peptides P2, P45 and P210. Animals received BSA-conjugated peptides or peptide-loaded bone marrow-derived dendritic cells (DCs). We used enzyme-linked immunosorbent assays to assess the synthesis of anti-peptide-specific IgG1 and IgG2a as well as the levels of interleukin (IL-)10 and interferon gamma (IFN-gamma) in plasma of immunised animals. We also measured the effect of immunisation on the number of spleen-derived CD4(+) and CD8(+) regulatory T cells (Tregs) in these animals. Peptide and peptide-loaded DC immunisation significantly increased the levels of peptide-specific immunoglobulins and the number of Tregs in apoE(-/-) mice. This was accompanied by a significant increase in the secretion of IL-10 with no effect on IFN-gamma levels. The results also show that the peptides can modulate the homing properties of DCs. Altogether, this study provides novel evidence for the immune mechanisms excerpted by apoB-100-derived peptides and their effect on Tregs and DCs relevant to their use in vaccine preparations. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/3843449
- author
- Pierides, Chrysoulla ; Bermudez-Fajardo, Alexandra ; Nordin Fredrikson, Gunilla LU ; Nilsson, Jan LU and Oviedo-Orta, Ernesto
- organization
- publishing date
- 2013
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Peptides, Dendritic cells, Regulatory T cells, Lymphocyte, Immune, response
- in
- Immunologic Research
- volume
- 56
- issue
- 1
- pages
- 96 - 108
- publisher
- Springer
- external identifiers
-
- wos:000317849100009
- scopus:84876417974
- pmid:23345063
- ISSN
- 0257-277X
- DOI
- 10.1007/s12026-013-8383-1
- language
- English
- LU publication?
- yes
- id
- 87a4a8e7-1844-4c03-a2b1-fade27806efd (old id 3843449)
- date added to LUP
- 2016-04-01 13:06:49
- date last changed
- 2022-02-04 02:30:17
@article{87a4a8e7-1844-4c03-a2b1-fade27806efd, abstract = {{Peptides derived from apolipoprotein B (apoB)-100 have been previously used in vaccine preparations to treat atherosclerosis. Such vaccines have been shown to reduce atherosclerotic plaque development by 50 % in experimental animals, and this effect is associated with induction of T helper (Th)2 immune responses. In this study we immunised apolipoprotein E-deficient (apoE(-/-)) mice with apoB-100-derived peptides P2, P45 and P210. Animals received BSA-conjugated peptides or peptide-loaded bone marrow-derived dendritic cells (DCs). We used enzyme-linked immunosorbent assays to assess the synthesis of anti-peptide-specific IgG1 and IgG2a as well as the levels of interleukin (IL-)10 and interferon gamma (IFN-gamma) in plasma of immunised animals. We also measured the effect of immunisation on the number of spleen-derived CD4(+) and CD8(+) regulatory T cells (Tregs) in these animals. Peptide and peptide-loaded DC immunisation significantly increased the levels of peptide-specific immunoglobulins and the number of Tregs in apoE(-/-) mice. This was accompanied by a significant increase in the secretion of IL-10 with no effect on IFN-gamma levels. The results also show that the peptides can modulate the homing properties of DCs. Altogether, this study provides novel evidence for the immune mechanisms excerpted by apoB-100-derived peptides and their effect on Tregs and DCs relevant to their use in vaccine preparations.}}, author = {{Pierides, Chrysoulla and Bermudez-Fajardo, Alexandra and Nordin Fredrikson, Gunilla and Nilsson, Jan and Oviedo-Orta, Ernesto}}, issn = {{0257-277X}}, keywords = {{Peptides; Dendritic cells; Regulatory T cells; Lymphocyte; Immune; response}}, language = {{eng}}, number = {{1}}, pages = {{96--108}}, publisher = {{Springer}}, series = {{Immunologic Research}}, title = {{Immune responses elicited by apoB-100-derived peptides in mice}}, url = {{http://dx.doi.org/10.1007/s12026-013-8383-1}}, doi = {{10.1007/s12026-013-8383-1}}, volume = {{56}}, year = {{2013}}, }