Protein Recovery Using Simulated Moving Bed Technology and Macroporous Chromatographic Support Material
(2007)- Abstract
- Due to its gentle and high resolving power, chromatography is the work horse within protein separation. Still it is an expensive method due to low productivity, diluted product streams, large waste streams and expensive support material. This limits its potential use to areas of high price products. Therefore there is a clear interest to make the technology cheaper to use both for the existing pharmaceutical applications but also to open doors to new areas like processing of effluents from the food industry.
The objective of this thesis work was to investigate the potential of simulated moving bed and a new macroporous chromatographic support material in the area of protein separation.
The studies... (More) - Due to its gentle and high resolving power, chromatography is the work horse within protein separation. Still it is an expensive method due to low productivity, diluted product streams, large waste streams and expensive support material. This limits its potential use to areas of high price products. Therefore there is a clear interest to make the technology cheaper to use both for the existing pharmaceutical applications but also to open doors to new areas like processing of effluents from the food industry.
The objective of this thesis work was to investigate the potential of simulated moving bed and a new macroporous chromatographic support material in the area of protein separation.
The studies presented in this thesis clearly demonstrate that simulated moving bed technology with its inherent coupling flexibility is a very interesting tool for protein isolation, both for individual as well as whole protein isolation. Efforts were made to compare the SMB-technology to single column set-ups in order to investigate whether stated benefits of the SMB could be shown. Perfectly fair comparisons are not easy to make since both processes must be indeed optimized and there should not be differences such as raw material and chromatographic support utilized. Still in the comparisons made it is rather clear that the positive effects of the SMB compared to single column chromatography, can be seen, especially the positive effects regarding buffer saving and product concentration.
The other way to improve process economy in chromatography addressed in this thesis was the development of new macroporous chromatographic support material. The macroporous monolithic pAAm-cryogels columns present attractive chromatographic media with unique properties like extremely low flow resistance. The results presented indicate that the important issue of reproducibility for these macroporous monoliths is controlled. Also, the potential of the macroporous monoliths to be used as a capturing column was demonstrated with satisfying results. The studies presented in this thesis are limited to the use of the macroporous material in protein separation. Still there are many possible applications for this new material, especially in the area of chromatographic separation of larger biomolecules and particles like plasmids, viruses and cells. Thus, the full potential of this new support remains to be elucidated. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/548145
- author
- Andersson, Jonatan LU
- supervisor
- opponent
-
- Associate professor Hobley, Timothy John, DTU
- organization
- publishing date
- 2007
- type
- Thesis
- publication status
- published
- subject
- keywords
- Bioteknik, Biotechnology, Chromatography, Simulated Moving Bed, Protein
- publisher
- Department of Biotechnology, Lund University
- defense location
- Center for Chemistry and Chemical Engineering, Lecture Hall B, Getingevägen 60, Lund University, Faculty of Engineering
- defense date
- 2007-03-20 10:30:00
- external identifiers
-
- other:ISRN: LUTKDH/TKBT-07/1105--SE
- ISBN
- 978-91-89627-52-9
- language
- English
- LU publication?
- yes
- additional info
- Jonatan Andersson and Bo Mattiasson. 2006. Simulated moving bed with a simplified approach for protein purification: Separation of lactoperoxidase and lactoferrin from whey protein concentrate. Journal of Chromatography A, vol 1107 pp 88-95. ElsevierJonatan Andersson, Deepti Sahoo and Bo Mattiasson. . Recovery of whey proteins isolate using simlated moving bed technology. (manuscript)Jonatan Andersson, Deepti Sahoo and Bo Mattiasson. . Isolation of potato protein by simulated moving bed technology. (submitted)Deepti Sahoo, Jonatan Andersson and Bo Mattiasson. . Immobilized metal affinity chromatography in simulated moving bed technology: Purification of a heat stable histidine tagged beta-glucosidase. (manuscript)Fatima Plieva, Jonatan Andersson, Igor Galaev and Bo Mattiasson. 2004. Characterization of polyacrylamide based monolithic columns. Journal of separation science, vol 27 pp 828-836. (manuscript)Ashok Kumar, Vibha Bansal, Jonatan Andersson, P Roychoudhury and Bo Mattiasson. 2006. Supermacroporous cryogel matrix for integrated protein isolation: Immobilized metal affinity chromatographic purification of urokinase from cell culture broth of a human kidney cell line. Journal of chromatography A, vol 1103 pp 35-42. Elsevier
- id
- 87c2dabd-147b-4fda-bbe8-1a6a999083c5 (old id 548145)
- date added to LUP
- 2016-04-04 11:59:20
- date last changed
- 2018-11-21 21:08:22
@phdthesis{87c2dabd-147b-4fda-bbe8-1a6a999083c5,
abstract = {{Due to its gentle and high resolving power, chromatography is the work horse within protein separation. Still it is an expensive method due to low productivity, diluted product streams, large waste streams and expensive support material. This limits its potential use to areas of high price products. Therefore there is a clear interest to make the technology cheaper to use both for the existing pharmaceutical applications but also to open doors to new areas like processing of effluents from the food industry.<br/><br>
<br/><br>
The objective of this thesis work was to investigate the potential of simulated moving bed and a new macroporous chromatographic support material in the area of protein separation.<br/><br>
<br/><br>
The studies presented in this thesis clearly demonstrate that simulated moving bed technology with its inherent coupling flexibility is a very interesting tool for protein isolation, both for individual as well as whole protein isolation. Efforts were made to compare the SMB-technology to single column set-ups in order to investigate whether stated benefits of the SMB could be shown. Perfectly fair comparisons are not easy to make since both processes must be indeed optimized and there should not be differences such as raw material and chromatographic support utilized. Still in the comparisons made it is rather clear that the positive effects of the SMB compared to single column chromatography, can be seen, especially the positive effects regarding buffer saving and product concentration.<br/><br>
<br/><br>
The other way to improve process economy in chromatography addressed in this thesis was the development of new macroporous chromatographic support material. The macroporous monolithic pAAm-cryogels columns present attractive chromatographic media with unique properties like extremely low flow resistance. The results presented indicate that the important issue of reproducibility for these macroporous monoliths is controlled. Also, the potential of the macroporous monoliths to be used as a capturing column was demonstrated with satisfying results. The studies presented in this thesis are limited to the use of the macroporous material in protein separation. Still there are many possible applications for this new material, especially in the area of chromatographic separation of larger biomolecules and particles like plasmids, viruses and cells. Thus, the full potential of this new support remains to be elucidated.}},
author = {{Andersson, Jonatan}},
isbn = {{978-91-89627-52-9}},
keywords = {{Bioteknik; Biotechnology; Chromatography; Simulated Moving Bed; Protein}},
language = {{eng}},
publisher = {{Department of Biotechnology, Lund University}},
school = {{Lund University}},
title = {{Protein Recovery Using Simulated Moving Bed Technology and Macroporous Chromatographic Support Material}},
year = {{2007}},
}