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Immunohistochemical Studies on Galectin Expression in Colectomised Patients with Ulcerative Colitis.

Block, Mattias; Mölne, Johan; Leffler, Hakon LU ; Börjesson, Lars and Breimer, Michael E (2016) In BioMed Research International 2016.
Abstract
Introduction. The aetiology and pathogenesis of ulcerative colitis (UC) are essentially unknown. Galectins are carbohydrate-binding lectins involved in a large number of physiological and pathophysiological processes. Little is known about the role of galectins in human UC. In this immunohistochemical exploratory study, both epithelial and inflammatory cell galectin expression were studied in patients with a thoroughly documented clinical history and were correlated with inflammatory activity. Material and Methods. Surgical whole intestinal wall colon specimens from UC patients (n = 22) and controls (n = 10) were studied. Clinical history, pharmacological treatment, and modified Mayo-score were recorded. Tissue inflammation was graded, and... (More)
Introduction. The aetiology and pathogenesis of ulcerative colitis (UC) are essentially unknown. Galectins are carbohydrate-binding lectins involved in a large number of physiological and pathophysiological processes. Little is known about the role of galectins in human UC. In this immunohistochemical exploratory study, both epithelial and inflammatory cell galectin expression were studied in patients with a thoroughly documented clinical history and were correlated with inflammatory activity. Material and Methods. Surgical whole intestinal wall colon specimens from UC patients (n = 22) and controls (n = 10) were studied. Clinical history, pharmacological treatment, and modified Mayo-score were recorded. Tissue inflammation was graded, and sections were stained with antibodies recognizing galectin-1, galectin-2, galectin-3, and galectin-4. Results. Galectin-1 was undetectable in normal and UC colonic epithelium, while galectin-2, galectin-3, and galectin-4 were strongly expressed. A tendency towards diminished epithelial expression with increased inflammatory grade for galectin-2, galectin-3, and galectin-4 was also found. In the inflammatory cells, a strong expression of galectin-2 and a weak expression of galectin-3 were seen. No clear-cut correlation between epithelial galectin expression and severity of the disease was found. Conclusion. Galectin expression in patients with UC seems to be more dependent on disease focality and individual variation than on degree of tissue inflammation. (Less)
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Contribution to journal
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published
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BioMed Research International
volume
2016
publisher
Hindawi Publishing Corporation
external identifiers
  • pmid:26885508
  • scopus:84958559474
  • wos:000369260400001
ISSN
2314-6133
DOI
10.1155/2016/5989128
language
English
LU publication?
yes
id
a09cf8c0-5297-4296-a316-6fcc7f88c00e (old id 8825235)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/26885508?dopt=Abstract
date added to LUP
2016-03-03 13:00:53
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2017-01-01 03:44:24
@article{a09cf8c0-5297-4296-a316-6fcc7f88c00e,
  abstract     = {Introduction. The aetiology and pathogenesis of ulcerative colitis (UC) are essentially unknown. Galectins are carbohydrate-binding lectins involved in a large number of physiological and pathophysiological processes. Little is known about the role of galectins in human UC. In this immunohistochemical exploratory study, both epithelial and inflammatory cell galectin expression were studied in patients with a thoroughly documented clinical history and were correlated with inflammatory activity. Material and Methods. Surgical whole intestinal wall colon specimens from UC patients (n = 22) and controls (n = 10) were studied. Clinical history, pharmacological treatment, and modified Mayo-score were recorded. Tissue inflammation was graded, and sections were stained with antibodies recognizing galectin-1, galectin-2, galectin-3, and galectin-4. Results. Galectin-1 was undetectable in normal and UC colonic epithelium, while galectin-2, galectin-3, and galectin-4 were strongly expressed. A tendency towards diminished epithelial expression with increased inflammatory grade for galectin-2, galectin-3, and galectin-4 was also found. In the inflammatory cells, a strong expression of galectin-2 and a weak expression of galectin-3 were seen. No clear-cut correlation between epithelial galectin expression and severity of the disease was found. Conclusion. Galectin expression in patients with UC seems to be more dependent on disease focality and individual variation than on degree of tissue inflammation.},
  articleno    = {5989128},
  author       = {Block, Mattias and Mölne, Johan and Leffler, Hakon and Börjesson, Lars and Breimer, Michael E},
  issn         = {2314-6133},
  language     = {eng},
  publisher    = {Hindawi Publishing Corporation},
  series       = {BioMed Research International},
  title        = {Immunohistochemical Studies on Galectin Expression in Colectomised Patients with Ulcerative Colitis.},
  url          = {http://dx.doi.org/10.1155/2016/5989128},
  volume       = {2016},
  year         = {2016},
}