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Increased availability of NADH in metabolically engineered baker's yeast improves transaminase-oxidoreductase coupled asymmetric whole-cell bioconversion.

Knudsen, Jan LU ; Hägglöf, Cecilia; Weber, Nora LU and Carlquist, Magnus LU (2016) In Microbial Cell Factories 15(1).
Abstract
Saccharomyces cerevisiae can be engineered to perform a multitude of different chemical reactions that are not programmed in its original genetic code. It has a large potential to function as whole-cell biocatalyst for one-pot multistep synthesis of various organic molecules, and it may thus serve as a powerful alternative or complement to traditional organic synthetic routes for new chemical entities (NCEs). However, although the selectivity in many cases is high, the catalytic activity is often low which results in low space-time-yields. In the case for NADH-dependent heterologous reductive reactions, a possible constraint is the availability of cytosolic NADH, which may be limited due to competition with native oxidative enzymes that... (More)
Saccharomyces cerevisiae can be engineered to perform a multitude of different chemical reactions that are not programmed in its original genetic code. It has a large potential to function as whole-cell biocatalyst for one-pot multistep synthesis of various organic molecules, and it may thus serve as a powerful alternative or complement to traditional organic synthetic routes for new chemical entities (NCEs). However, although the selectivity in many cases is high, the catalytic activity is often low which results in low space-time-yields. In the case for NADH-dependent heterologous reductive reactions, a possible constraint is the availability of cytosolic NADH, which may be limited due to competition with native oxidative enzymes that act to maintain redox homeostasis. In this study, the effect of increasing the availability of cytosolic NADH on the catalytic activity of engineered yeast for transamination-reduction coupled asymmetric one-pot conversion was investigated. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Microbial Cell Factories
volume
15
issue
1
publisher
BioMed Central
external identifiers
  • pmid:26879378
  • scopus:84957956469
  • wos:000370027700002
ISSN
1475-2859
DOI
10.1186/s12934-016-0430-x
language
English
LU publication?
yes
id
572c5aa7-a979-4e1c-9eb2-4ff5e9986f68 (old id 8825433)
date added to LUP
2016-03-07 16:14:00
date last changed
2017-02-05 04:11:07
@article{572c5aa7-a979-4e1c-9eb2-4ff5e9986f68,
  abstract     = {Saccharomyces cerevisiae can be engineered to perform a multitude of different chemical reactions that are not programmed in its original genetic code. It has a large potential to function as whole-cell biocatalyst for one-pot multistep synthesis of various organic molecules, and it may thus serve as a powerful alternative or complement to traditional organic synthetic routes for new chemical entities (NCEs). However, although the selectivity in many cases is high, the catalytic activity is often low which results in low space-time-yields. In the case for NADH-dependent heterologous reductive reactions, a possible constraint is the availability of cytosolic NADH, which may be limited due to competition with native oxidative enzymes that act to maintain redox homeostasis. In this study, the effect of increasing the availability of cytosolic NADH on the catalytic activity of engineered yeast for transamination-reduction coupled asymmetric one-pot conversion was investigated.},
  articleno    = {37},
  author       = {Knudsen, Jan and Hägglöf, Cecilia and Weber, Nora and Carlquist, Magnus},
  issn         = {1475-2859},
  language     = {eng},
  number       = {1},
  publisher    = {BioMed Central},
  series       = {Microbial Cell Factories},
  title        = {Increased availability of NADH in metabolically engineered baker's yeast improves transaminase-oxidoreductase coupled asymmetric whole-cell bioconversion.},
  url          = {http://dx.doi.org/10.1186/s12934-016-0430-x},
  volume       = {15},
  year         = {2016},
}