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Short communication: Lactic acid bacteria from the honeybee inhibit the in vitro growth of mastitis pathogens.

Piccart, K; Vasquez, Alejandra LU ; Piepers, S; De Vliegher, S and Olofsson, Tobias LU (2016) In Journal of Dairy Science
Abstract
Despite the increasing knowledge of prevention and control strategies, bovine mastitis remains one of the most challenging diseases in the dairy industry. This study investigated the antimicrobial activity of 13 species of lactic acid bacteria (LAB), previously isolated from the honey crop of the honeybee, on several mastitis pathogens. The viable LAB were first reintroduced into a sterilized heather honey matrix. More than 20 different bovine mastitis isolates were tested against the mixture of the 13 LAB species in the honey medium using a dual-culture overlay assay. The mastitis isolates were identified through bacteriological culturing, followed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.... (More)
Despite the increasing knowledge of prevention and control strategies, bovine mastitis remains one of the most challenging diseases in the dairy industry. This study investigated the antimicrobial activity of 13 species of lactic acid bacteria (LAB), previously isolated from the honey crop of the honeybee, on several mastitis pathogens. The viable LAB were first reintroduced into a sterilized heather honey matrix. More than 20 different bovine mastitis isolates were tested against the mixture of the 13 LAB species in the honey medium using a dual-culture overlay assay. The mastitis isolates were identified through bacteriological culturing, followed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Additionally, the mastitis isolates were subjected to antimicrobial susceptibility testing through disk diffusion. Growth of all tested mastitis pathogens, including the ones displaying antimicrobial resistance to one or more antimicrobial compounds, were inhibited to some extent by the honey and LAB combination. The antibacterial effect of these LAB opens up new perspectives on alternative treatment and prevention of bovine mastitis. (Less)
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organization
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Contribution to journal
publication status
published
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in
Journal of Dairy Science
publisher
American Dairy Science Association
external identifiers
  • pmid:26830735
  • scopus:84955564277
  • wos:000372945500048
ISSN
1525-3198
DOI
10.3168/jds.2015-10208
language
English
LU publication?
yes
id
65d8b924-0897-4f6f-adc7-075acfb317e7 (old id 8830074)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/26830735?dopt=Abstract
date added to LUP
2016-03-01 11:45:17
date last changed
2017-08-27 03:27:13
@article{65d8b924-0897-4f6f-adc7-075acfb317e7,
  abstract     = {Despite the increasing knowledge of prevention and control strategies, bovine mastitis remains one of the most challenging diseases in the dairy industry. This study investigated the antimicrobial activity of 13 species of lactic acid bacteria (LAB), previously isolated from the honey crop of the honeybee, on several mastitis pathogens. The viable LAB were first reintroduced into a sterilized heather honey matrix. More than 20 different bovine mastitis isolates were tested against the mixture of the 13 LAB species in the honey medium using a dual-culture overlay assay. The mastitis isolates were identified through bacteriological culturing, followed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Additionally, the mastitis isolates were subjected to antimicrobial susceptibility testing through disk diffusion. Growth of all tested mastitis pathogens, including the ones displaying antimicrobial resistance to one or more antimicrobial compounds, were inhibited to some extent by the honey and LAB combination. The antibacterial effect of these LAB opens up new perspectives on alternative treatment and prevention of bovine mastitis.},
  author       = {Piccart, K and Vasquez, Alejandra and Piepers, S and De Vliegher, S and Olofsson, Tobias},
  issn         = {1525-3198},
  language     = {eng},
  month        = {01},
  publisher    = {American Dairy Science Association},
  series       = {Journal of Dairy Science},
  title        = {Short communication: Lactic acid bacteria from the honeybee inhibit the in vitro growth of mastitis pathogens.},
  url          = {http://dx.doi.org/10.3168/jds.2015-10208},
  year         = {2016},
}