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Optogenetic Control of Pancreatic Islets.

Reinbothe, Thomas M and Mollet, Ines LU (2016) In Optogenetics. Methods and Protocols. 1408. p.107-123
Abstract
In light of the emerging diabetes epidemic, new experimental approaches in islet research are needed to elucidate the mechanisms behind pancreatic islet dysfunction and to facilitate the development of more effective therapies. Optogenetics has created numerous new experimental tools enabling us to gain insights into processes little was known about before. The spatial and temporal precision that it can achieve is also attractive for studying the cells of the pancreatic islet and we set out to explore the possibilities of this technology for our purposes. We here describe how to use the islets of an "optogenetic beta-cell" mouse line in islet batch incubations and Ca(2+) imaging experiments. This protocol enables light-induced insulin... (More)
In light of the emerging diabetes epidemic, new experimental approaches in islet research are needed to elucidate the mechanisms behind pancreatic islet dysfunction and to facilitate the development of more effective therapies. Optogenetics has created numerous new experimental tools enabling us to gain insights into processes little was known about before. The spatial and temporal precision that it can achieve is also attractive for studying the cells of the pancreatic islet and we set out to explore the possibilities of this technology for our purposes. We here describe how to use the islets of an "optogenetic beta-cell" mouse line in islet batch incubations and Ca(2+) imaging experiments. This protocol enables light-induced insulin release and provides an all-optical solution to control and measure intracellular Ca(2+) levels in pancreatic beta-cells. The technique is easy to set up and provides a useful tool for controlling the activity of distinct islet cell populations. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
in
Optogenetics. Methods and Protocols.
editor
Kianianmomeni, Arash and
volume
1408
pages
107 - 123
publisher
Springer
external identifiers
  • pmid:26965119
  • scopus:84961249429
ISSN
1064-3745
1940-6029
ISBN
978-1-4939-3510-9
DOI
10.1007/978-1-4939-3512-3_8
language
English
LU publication?
yes
id
55630cde-4f7b-43b4-b6d8-412daa61f33e (old id 8852734)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/26965119?dopt=Abstract
date added to LUP
2016-03-15 15:00:52
date last changed
2017-01-01 03:25:18
@inbook{55630cde-4f7b-43b4-b6d8-412daa61f33e,
  abstract     = {In light of the emerging diabetes epidemic, new experimental approaches in islet research are needed to elucidate the mechanisms behind pancreatic islet dysfunction and to facilitate the development of more effective therapies. Optogenetics has created numerous new experimental tools enabling us to gain insights into processes little was known about before. The spatial and temporal precision that it can achieve is also attractive for studying the cells of the pancreatic islet and we set out to explore the possibilities of this technology for our purposes. We here describe how to use the islets of an "optogenetic beta-cell" mouse line in islet batch incubations and Ca(2+) imaging experiments. This protocol enables light-induced insulin release and provides an all-optical solution to control and measure intracellular Ca(2+) levels in pancreatic beta-cells. The technique is easy to set up and provides a useful tool for controlling the activity of distinct islet cell populations.},
  author       = {Reinbothe, Thomas M and Mollet, Ines},
  editor       = {Kianianmomeni, Arash},
  isbn         = {978-1-4939-3510-9},
  issn         = {1064-3745},
  language     = {eng},
  pages        = {107--123},
  publisher    = {Springer},
  series       = {Optogenetics. Methods and Protocols.},
  title        = {Optogenetic Control of Pancreatic Islets.},
  url          = {http://dx.doi.org/10.1007/978-1-4939-3512-3_8},
  volume       = {1408},
  year         = {2016},
}