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Context-Dependent Development of Lymphoid Stroma from Adult CD34(+) Adventitial Progenitors.

Sitnik, Katarzyna M; Wendland, Kerstin LU ; Weishaupt, Holger; Uronen-Hansson, Heli LU ; White, Andrea J; Anderson, Graham; Kotarsky, Knut LU and Agace, William W (2016) In Cell Reports 14(10). p.2375-2388
Abstract
Despite the key role of primary and secondary lymphoid organ stroma in immunity, our understanding of the heterogeneity and ontogeny of these cells remains limited. Here, we identify a functionally distinct subset of BP3(-)PDPN(+)PDGFRβ(+)/α(+)CD34(+) stromal adventitial cells in both lymph nodes (LNs) and thymus that is located within the vascular niche surrounding PDPN(-)PDGFRβ(+)/α(-)Esam-1(+)ITGA7(+) pericytes. CD34(+) adventitial cells developed in late embryonic thymus and in postnatal LNs and in the thymus originated, along with pericytes, from a common anlage-seeding progenitor population. Using lymphoid organ re-aggregate grafts, we demonstrate that adult CD34(+) adventitial cells are capable of differentiating into multiple... (More)
Despite the key role of primary and secondary lymphoid organ stroma in immunity, our understanding of the heterogeneity and ontogeny of these cells remains limited. Here, we identify a functionally distinct subset of BP3(-)PDPN(+)PDGFRβ(+)/α(+)CD34(+) stromal adventitial cells in both lymph nodes (LNs) and thymus that is located within the vascular niche surrounding PDPN(-)PDGFRβ(+)/α(-)Esam-1(+)ITGA7(+) pericytes. CD34(+) adventitial cells developed in late embryonic thymus and in postnatal LNs and in the thymus originated, along with pericytes, from a common anlage-seeding progenitor population. Using lymphoid organ re-aggregate grafts, we demonstrate that adult CD34(+) adventitial cells are capable of differentiating into multiple lymphoid stroma-like subsets including pericyte-, FRC-, MRC-, and FDC-like cells, the development of which was lymphoid environment-dependent. These findings extend the current understanding of lymphoid mesenchymal cell heterogeneity and highlight a role of the CD34(+) adventitia as a potential ubiquitous source of lymphoid stromal precursors in postnatal tissues. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Cell Reports
volume
14
issue
10
pages
2375 - 2388
publisher
Cell Press
external identifiers
  • pmid:26947077
  • scopus:84960436706
  • wos:000371998700010
ISSN
2211-1247
DOI
10.1016/j.celrep.2016.02.033
language
English
LU publication?
yes
id
0fd6cc07-7048-4b6c-82bb-f71569e799c3 (old id 8856076)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/26947077?dopt=Abstract
date added to LUP
2016-03-15 12:09:55
date last changed
2017-10-01 05:02:55
@article{0fd6cc07-7048-4b6c-82bb-f71569e799c3,
  abstract     = {Despite the key role of primary and secondary lymphoid organ stroma in immunity, our understanding of the heterogeneity and ontogeny of these cells remains limited. Here, we identify a functionally distinct subset of BP3(-)PDPN(+)PDGFRβ(+)/α(+)CD34(+) stromal adventitial cells in both lymph nodes (LNs) and thymus that is located within the vascular niche surrounding PDPN(-)PDGFRβ(+)/α(-)Esam-1(+)ITGA7(+) pericytes. CD34(+) adventitial cells developed in late embryonic thymus and in postnatal LNs and in the thymus originated, along with pericytes, from a common anlage-seeding progenitor population. Using lymphoid organ re-aggregate grafts, we demonstrate that adult CD34(+) adventitial cells are capable of differentiating into multiple lymphoid stroma-like subsets including pericyte-, FRC-, MRC-, and FDC-like cells, the development of which was lymphoid environment-dependent. These findings extend the current understanding of lymphoid mesenchymal cell heterogeneity and highlight a role of the CD34(+) adventitia as a potential ubiquitous source of lymphoid stromal precursors in postnatal tissues.},
  author       = {Sitnik, Katarzyna M and Wendland, Kerstin and Weishaupt, Holger and Uronen-Hansson, Heli and White, Andrea J and Anderson, Graham and Kotarsky, Knut and Agace, William W},
  issn         = {2211-1247},
  language     = {eng},
  month        = {03},
  number       = {10},
  pages        = {2375--2388},
  publisher    = {Cell Press},
  series       = {Cell Reports},
  title        = {Context-Dependent Development of Lymphoid Stroma from Adult CD34(+) Adventitial Progenitors.},
  url          = {http://dx.doi.org/10.1016/j.celrep.2016.02.033},
  volume       = {14},
  year         = {2016},
}