Context-Dependent Development of Lymphoid Stroma from Adult CD34(+) Adventitial Progenitors.
(2016) In Cell Reports 14(10). p.2375-2388- Abstract
- Despite the key role of primary and secondary lymphoid organ stroma in immunity, our understanding of the heterogeneity and ontogeny of these cells remains limited. Here, we identify a functionally distinct subset of BP3(-)PDPN(+)PDGFRβ(+)/α(+)CD34(+) stromal adventitial cells in both lymph nodes (LNs) and thymus that is located within the vascular niche surrounding PDPN(-)PDGFRβ(+)/α(-)Esam-1(+)ITGA7(+) pericytes. CD34(+) adventitial cells developed in late embryonic thymus and in postnatal LNs and in the thymus originated, along with pericytes, from a common anlage-seeding progenitor population. Using lymphoid organ re-aggregate grafts, we demonstrate that adult CD34(+) adventitial cells are capable of differentiating into multiple... (More)
- Despite the key role of primary and secondary lymphoid organ stroma in immunity, our understanding of the heterogeneity and ontogeny of these cells remains limited. Here, we identify a functionally distinct subset of BP3(-)PDPN(+)PDGFRβ(+)/α(+)CD34(+) stromal adventitial cells in both lymph nodes (LNs) and thymus that is located within the vascular niche surrounding PDPN(-)PDGFRβ(+)/α(-)Esam-1(+)ITGA7(+) pericytes. CD34(+) adventitial cells developed in late embryonic thymus and in postnatal LNs and in the thymus originated, along with pericytes, from a common anlage-seeding progenitor population. Using lymphoid organ re-aggregate grafts, we demonstrate that adult CD34(+) adventitial cells are capable of differentiating into multiple lymphoid stroma-like subsets including pericyte-, FRC-, MRC-, and FDC-like cells, the development of which was lymphoid environment-dependent. These findings extend the current understanding of lymphoid mesenchymal cell heterogeneity and highlight a role of the CD34(+) adventitia as a potential ubiquitous source of lymphoid stromal precursors in postnatal tissues. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/8856076
- author
- Sitnik, Katarzyna M ; Wendland, Kerstin LU ; Weishaupt, Holger ; Uronen-Hansson, Heli LU ; White, Andrea J ; Anderson, Graham ; Kotarsky, Knut LU and Agace, William W
- organization
- publishing date
- 2016-03-02
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Cell Reports
- volume
- 14
- issue
- 10
- pages
- 2375 - 2388
- publisher
- Cell Press
- external identifiers
-
- pmid:26947077
- scopus:84960436706
- wos:000371998700010
- pmid:26947077
- ISSN
- 2211-1247
- DOI
- 10.1016/j.celrep.2016.02.033
- language
- English
- LU publication?
- yes
- id
- 0fd6cc07-7048-4b6c-82bb-f71569e799c3 (old id 8856076)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/26947077?dopt=Abstract
- date added to LUP
- 2016-04-04 09:20:55
- date last changed
- 2022-04-23 20:07:50
@article{0fd6cc07-7048-4b6c-82bb-f71569e799c3, abstract = {{Despite the key role of primary and secondary lymphoid organ stroma in immunity, our understanding of the heterogeneity and ontogeny of these cells remains limited. Here, we identify a functionally distinct subset of BP3(-)PDPN(+)PDGFRβ(+)/α(+)CD34(+) stromal adventitial cells in both lymph nodes (LNs) and thymus that is located within the vascular niche surrounding PDPN(-)PDGFRβ(+)/α(-)Esam-1(+)ITGA7(+) pericytes. CD34(+) adventitial cells developed in late embryonic thymus and in postnatal LNs and in the thymus originated, along with pericytes, from a common anlage-seeding progenitor population. Using lymphoid organ re-aggregate grafts, we demonstrate that adult CD34(+) adventitial cells are capable of differentiating into multiple lymphoid stroma-like subsets including pericyte-, FRC-, MRC-, and FDC-like cells, the development of which was lymphoid environment-dependent. These findings extend the current understanding of lymphoid mesenchymal cell heterogeneity and highlight a role of the CD34(+) adventitia as a potential ubiquitous source of lymphoid stromal precursors in postnatal tissues.}}, author = {{Sitnik, Katarzyna M and Wendland, Kerstin and Weishaupt, Holger and Uronen-Hansson, Heli and White, Andrea J and Anderson, Graham and Kotarsky, Knut and Agace, William W}}, issn = {{2211-1247}}, language = {{eng}}, month = {{03}}, number = {{10}}, pages = {{2375--2388}}, publisher = {{Cell Press}}, series = {{Cell Reports}}, title = {{Context-Dependent Development of Lymphoid Stroma from Adult CD34(+) Adventitial Progenitors.}}, url = {{http://dx.doi.org/10.1016/j.celrep.2016.02.033}}, doi = {{10.1016/j.celrep.2016.02.033}}, volume = {{14}}, year = {{2016}}, }