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Immunocapillarymigration with enzyme-labeled antibodies : rapid quantification of C-reactive protein in human plasma

Glad, C and Grubb, A O LU orcid (1981) In Analytical Biochemistry 116(2). p.40-335
Abstract
Various procedures to improve the sensitivity and precision of antigen quantitation by immunocapillarymigration are investigated. The best results are obtained when using porous strips of cellulose acetate with covalently attached antibodies and when enzyme-labeled antibodies are used to expose the antigen-covered areas of the strips. Such a system has a sensitivity of 0.15 mg/liter and a precision of 7%. It allows a rapid quantitation of human C-reactive protein without the use of laboratory instrumentation.
Please use this url to cite or link to this publication:
author
and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
C-Reactive Protein/analysis, Capillary Action, Cellulose/analogs & derivatives, Drug Stability, Humans, Immunoelectrophoresis, Immunoenzyme Techniques/standards
in
Analytical Biochemistry
volume
116
issue
2
pages
40 - 335
publisher
Elsevier
external identifiers
  • pmid:7032355
  • scopus:0019730671
ISSN
0003-2697
DOI
10.1016/0003-2697(81)90367-5
language
English
LU publication?
yes
id
8857734b-3d18-4472-b966-024e581fe3f1
date added to LUP
2021-10-21 16:23:25
date last changed
2024-01-20 15:33:10
@article{8857734b-3d18-4472-b966-024e581fe3f1,
  abstract     = {{Various procedures to improve the sensitivity and precision of antigen quantitation by immunocapillarymigration are investigated. The best results are obtained when using porous strips of cellulose acetate with covalently attached antibodies and when enzyme-labeled antibodies are used to expose the antigen-covered areas of the strips. Such a system has a sensitivity of 0.15 mg/liter and a precision of 7%. It allows a rapid quantitation of human C-reactive protein without the use of laboratory instrumentation.}},
  author       = {{Glad, C and Grubb, A O}},
  issn         = {{0003-2697}},
  keywords     = {{C-Reactive Protein/analysis; Capillary Action; Cellulose/analogs & derivatives; Drug Stability; Humans; Immunoelectrophoresis; Immunoenzyme Techniques/standards}},
  language     = {{eng}},
  number       = {{2}},
  pages        = {{40--335}},
  publisher    = {{Elsevier}},
  series       = {{Analytical Biochemistry}},
  title        = {{Immunocapillarymigration with enzyme-labeled antibodies : rapid quantification of C-reactive protein in human plasma}},
  url          = {{http://dx.doi.org/10.1016/0003-2697(81)90367-5}},
  doi          = {{10.1016/0003-2697(81)90367-5}},
  volume       = {{116}},
  year         = {{1981}},
}