Immunocapillarymigration with enzyme-labeled antibodies : rapid quantification of C-reactive protein in human plasma
(1981) In Analytical Biochemistry 116(2). p.40-335- Abstract
- Various procedures to improve the sensitivity and precision of antigen quantitation by immunocapillarymigration are investigated. The best results are obtained when using porous strips of cellulose acetate with covalently attached antibodies and when enzyme-labeled antibodies are used to expose the antigen-covered areas of the strips. Such a system has a sensitivity of 0.15 mg/liter and a precision of 7%. It allows a rapid quantitation of human C-reactive protein without the use of laboratory instrumentation.
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https://lup.lub.lu.se/record/8857734b-3d18-4472-b966-024e581fe3f1
- author
- Glad, C
and Grubb, A O
LU
- organization
- publishing date
- 1981
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- C-Reactive Protein/analysis, Capillary Action, Cellulose/analogs & derivatives, Drug Stability, Humans, Immunoelectrophoresis, Immunoenzyme Techniques/standards
- in
- Analytical Biochemistry
- volume
- 116
- issue
- 2
- pages
- 40 - 335
- publisher
- Elsevier
- external identifiers
-
- pmid:7032355
- scopus:0019730671
- ISSN
- 0003-2697
- DOI
- 10.1016/0003-2697(81)90367-5
- language
- English
- LU publication?
- yes
- id
- 8857734b-3d18-4472-b966-024e581fe3f1
- date added to LUP
- 2021-10-21 16:23:25
- date last changed
- 2024-01-20 15:33:10
@article{8857734b-3d18-4472-b966-024e581fe3f1, abstract = {{Various procedures to improve the sensitivity and precision of antigen quantitation by immunocapillarymigration are investigated. The best results are obtained when using porous strips of cellulose acetate with covalently attached antibodies and when enzyme-labeled antibodies are used to expose the antigen-covered areas of the strips. Such a system has a sensitivity of 0.15 mg/liter and a precision of 7%. It allows a rapid quantitation of human C-reactive protein without the use of laboratory instrumentation.}}, author = {{Glad, C and Grubb, A O}}, issn = {{0003-2697}}, keywords = {{C-Reactive Protein/analysis; Capillary Action; Cellulose/analogs & derivatives; Drug Stability; Humans; Immunoelectrophoresis; Immunoenzyme Techniques/standards}}, language = {{eng}}, number = {{2}}, pages = {{40--335}}, publisher = {{Elsevier}}, series = {{Analytical Biochemistry}}, title = {{Immunocapillarymigration with enzyme-labeled antibodies : rapid quantification of C-reactive protein in human plasma}}, url = {{http://dx.doi.org/10.1016/0003-2697(81)90367-5}}, doi = {{10.1016/0003-2697(81)90367-5}}, volume = {{116}}, year = {{1981}}, }