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Effect of wet storage conditions on potato tuber transcriptome, phytohormones and growth

Peivastegan, Bahram; Hadizadeh, Iman; Nykyri, Johanna; Nielsen, Kåre Lehmann; Somervuo, Panu; Sipari, Nina; Tran, Cuong LU and Pirhonen, Minna (2019) In BMC Plant Biology 19(1).
Abstract

Background: Stored potato (Solanum tuberosum L.) tubers are sensitive to wet conditions that can cause rotting in long-term storage. To study the effect of water on the tuber surface during storage, microarray analysis, RNA-Seq profiling, qRT-PCR and phytohormone measurements were performed to study gene expression and hormone content in wet tubers incubated at two temperatures: 4 °C and 15 °C. The growth of the plants was also observed in a greenhouse after the incubation of tubers in wet conditions. Results: Wet conditions induced a low-oxygen response, suggesting reduced oxygen availability in wet tubers at both temperatures when compared to that in the corresponding dry samples. Wet conditions induced genes coding for heat shock... (More)

Background: Stored potato (Solanum tuberosum L.) tubers are sensitive to wet conditions that can cause rotting in long-term storage. To study the effect of water on the tuber surface during storage, microarray analysis, RNA-Seq profiling, qRT-PCR and phytohormone measurements were performed to study gene expression and hormone content in wet tubers incubated at two temperatures: 4 °C and 15 °C. The growth of the plants was also observed in a greenhouse after the incubation of tubers in wet conditions. Results: Wet conditions induced a low-oxygen response, suggesting reduced oxygen availability in wet tubers at both temperatures when compared to that in the corresponding dry samples. Wet conditions induced genes coding for heat shock proteins, as well as proteins involved in fermentative energy production and defense against reactive oxygen species (ROS), which are transcripts that have been previously associated with low-oxygen stress in hypoxic or anoxic conditions. Wet treatment also induced senescence-related gene expression and genes involved in cell wall loosening, but downregulated genes encoding protease inhibitors and proteins involved in chloroplast functions and in the biosynthesis of secondary metabolites. Many genes involved in the production of phytohormones and signaling were also affected by wet conditions, suggesting altered regulation of growth by wet conditions. Hormone measurements after incubation showed increased salicylic acid (SA), abscisic acid (ABA) and auxin (IAA) concentrations as well as reduced production of jasmonate 12-oxo-phytodienoic acid (OPDA) in wet tubers. After incubation in wet conditions, the tubers produced fewer stems and more roots compared to controls incubated in dry conditions. Conclusions: In wet conditions, tubers invest in ROS protection and defense against the abiotic stress caused by reduced oxygen due to excessive water. Changes in ABA, SA and IAA that are antagonistic to jasmonates affect growth and defenses, causing induction of root growth and rendering tubers susceptible to necrotrophic pathogens. Water on the tuber surface may function as a signal for growth, similar to germination of seeds.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Defence, Energy metabolism, Growth, Microarray, Oxygen, Potato, RNA-Seq, Storage, Tuber, Water
in
BMC Plant Biology
volume
19
issue
1
publisher
BioMed Central
external identifiers
  • scopus:85067429639
ISSN
1471-2229
DOI
10.1186/s12870-019-1875-y
language
English
LU publication?
yes
id
88a97f03-6710-4c7f-a474-9cb5d600a445
date added to LUP
2019-07-05 12:14:37
date last changed
2019-07-30 05:06:06
@article{88a97f03-6710-4c7f-a474-9cb5d600a445,
  abstract     = {<p>Background: Stored potato (Solanum tuberosum L.) tubers are sensitive to wet conditions that can cause rotting in long-term storage. To study the effect of water on the tuber surface during storage, microarray analysis, RNA-Seq profiling, qRT-PCR and phytohormone measurements were performed to study gene expression and hormone content in wet tubers incubated at two temperatures: 4 °C and 15 °C. The growth of the plants was also observed in a greenhouse after the incubation of tubers in wet conditions. Results: Wet conditions induced a low-oxygen response, suggesting reduced oxygen availability in wet tubers at both temperatures when compared to that in the corresponding dry samples. Wet conditions induced genes coding for heat shock proteins, as well as proteins involved in fermentative energy production and defense against reactive oxygen species (ROS), which are transcripts that have been previously associated with low-oxygen stress in hypoxic or anoxic conditions. Wet treatment also induced senescence-related gene expression and genes involved in cell wall loosening, but downregulated genes encoding protease inhibitors and proteins involved in chloroplast functions and in the biosynthesis of secondary metabolites. Many genes involved in the production of phytohormones and signaling were also affected by wet conditions, suggesting altered regulation of growth by wet conditions. Hormone measurements after incubation showed increased salicylic acid (SA), abscisic acid (ABA) and auxin (IAA) concentrations as well as reduced production of jasmonate 12-oxo-phytodienoic acid (OPDA) in wet tubers. After incubation in wet conditions, the tubers produced fewer stems and more roots compared to controls incubated in dry conditions. Conclusions: In wet conditions, tubers invest in ROS protection and defense against the abiotic stress caused by reduced oxygen due to excessive water. Changes in ABA, SA and IAA that are antagonistic to jasmonates affect growth and defenses, causing induction of root growth and rendering tubers susceptible to necrotrophic pathogens. Water on the tuber surface may function as a signal for growth, similar to germination of seeds.</p>},
  articleno    = {262},
  author       = {Peivastegan, Bahram and Hadizadeh, Iman and Nykyri, Johanna and Nielsen, Kåre Lehmann and Somervuo, Panu and Sipari, Nina and Tran, Cuong and Pirhonen, Minna},
  issn         = {1471-2229},
  keyword      = {Defence,Energy metabolism,Growth,Microarray,Oxygen,Potato,RNA-Seq,Storage,Tuber,Water},
  language     = {eng},
  number       = {1},
  publisher    = {BioMed Central},
  series       = {BMC Plant Biology},
  title        = {Effect of wet storage conditions on potato tuber transcriptome, phytohormones and growth},
  url          = {http://dx.doi.org/10.1186/s12870-019-1875-y},
  volume       = {19},
  year         = {2019},
}