Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

Effects of reactive oxygen species generation induced by Wonju City particulate matter on mitochondrial dysfunction in human middle ear cell

Lee, Su Hoon ; Ha, Sun Mok ; Jeong, Min Jae ; Park, Dong Jun ; Polo, Carlos Noriega LU orcid ; Seo, Young Joon and Kim, Seong Heon (2021) In Environmental Science and Pollution Research 28(35). p.49244-49257
Abstract

Atmospheric particulate matter (PM) contains different components that can elicit varying adverse health effects in humans and animals. Studies on PM toxicity and its underlying mechanisms in the middle ear are limited, and they generally use a PM standard. However, as PM composition varies temporally and geographically, it is crucial to identify the toxic PM constituents according to season and region and investigate their associated health effects. Thus, we sought to determine whether PM induces cytotoxicity and inflammatory factor and reactive oxygen species (ROS) generation in human middle ear epithelial cells obtained from patients with otitis media. The cells were treated with both standard urban PM and PM directly captured from... (More)

Atmospheric particulate matter (PM) contains different components that can elicit varying adverse health effects in humans and animals. Studies on PM toxicity and its underlying mechanisms in the middle ear are limited, and they generally use a PM standard. However, as PM composition varies temporally and geographically, it is crucial to identify the toxic PM constituents according to season and region and investigate their associated health effects. Thus, we sought to determine whether PM induces cytotoxicity and inflammatory factor and reactive oxygen species (ROS) generation in human middle ear epithelial cells obtained from patients with otitis media. The cells were treated with both standard urban PM and PM directly captured from the atmosphere in Wonju City. The association between mitochondrial dysfunction and PM was investigated. PM exposure significantly increased COX-2 and TNF-α mRNA expression, increased ROS generation, induced inflammatory responses, and caused abnormalities in mitochondrial motility and function. Furthermore, PM induced cell apoptosis, which consequently reduced cell survival, particularly at the concentration of 100 μg/mL. Overall, our study provides new insights into the toxic effects of standard and atmospheric PM on middle ear cell line.

(Less)
Please use this url to cite or link to this publication:
author
; ; ; ; ; and
publishing date
type
Contribution to journal
publication status
published
keywords
Air Pollutants/analysis, Animals, Cities, Ear, Middle/chemistry, Humans, Mitochondria/chemistry, Oxidative Stress, Particulate Matter/analysis, Reactive Oxygen Species
in
Environmental Science and Pollution Research
volume
28
issue
35
pages
49244 - 49257
publisher
Springer
external identifiers
  • pmid:33932209
  • scopus:85105390813
ISSN
1614-7499
DOI
10.1007/s11356-021-14216-7
language
English
LU publication?
no
additional info
© 2021. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.
id
88dc0078-1905-4b5f-b805-5e5f83a2fff0
date added to LUP
2026-01-29 09:51:16
date last changed
2026-01-30 04:01:46
@article{88dc0078-1905-4b5f-b805-5e5f83a2fff0,
  abstract     = {{<p>Atmospheric particulate matter (PM) contains different components that can elicit varying adverse health effects in humans and animals. Studies on PM toxicity and its underlying mechanisms in the middle ear are limited, and they generally use a PM standard. However, as PM composition varies temporally and geographically, it is crucial to identify the toxic PM constituents according to season and region and investigate their associated health effects. Thus, we sought to determine whether PM induces cytotoxicity and inflammatory factor and reactive oxygen species (ROS) generation in human middle ear epithelial cells obtained from patients with otitis media. The cells were treated with both standard urban PM and PM directly captured from the atmosphere in Wonju City. The association between mitochondrial dysfunction and PM was investigated. PM exposure significantly increased COX-2 and TNF-α mRNA expression, increased ROS generation, induced inflammatory responses, and caused abnormalities in mitochondrial motility and function. Furthermore, PM induced cell apoptosis, which consequently reduced cell survival, particularly at the concentration of 100 μg/mL. Overall, our study provides new insights into the toxic effects of standard and atmospheric PM on middle ear cell line.</p>}},
  author       = {{Lee, Su Hoon and Ha, Sun Mok and Jeong, Min Jae and Park, Dong Jun and Polo, Carlos Noriega and Seo, Young Joon and Kim, Seong Heon}},
  issn         = {{1614-7499}},
  keywords     = {{Air Pollutants/analysis; Animals; Cities; Ear, Middle/chemistry; Humans; Mitochondria/chemistry; Oxidative Stress; Particulate Matter/analysis; Reactive Oxygen Species}},
  language     = {{eng}},
  number       = {{35}},
  pages        = {{49244--49257}},
  publisher    = {{Springer}},
  series       = {{Environmental Science and Pollution Research}},
  title        = {{Effects of reactive oxygen species generation induced by Wonju City particulate matter on mitochondrial dysfunction in human middle ear cell}},
  url          = {{http://dx.doi.org/10.1007/s11356-021-14216-7}},
  doi          = {{10.1007/s11356-021-14216-7}},
  volume       = {{28}},
  year         = {{2021}},
}