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Characterisation of a flavonoid ligand of the fungal protein Alt a 1

Garrido-Arandia, Mariá; Silva-Navas, Javier; Ramírez-Castillejo, Carmen; Cubells-Baeza, Nuria; Gómez-Casado, Cristina LU ; Barber, Domingo; Pozo, Juan C.; Melendi, Pablo G.; Pacios, Luis F. and Diáz-Perales, Araceli (2016) In Scientific Reports 6.
Abstract

Spores of pathogenic fungi are virtually ubiquitous and cause human disease and severe losses in crops. The endophytic fungi Alternaria species produce host-selective phytotoxins. Alt a 1 is a strongly allergenic protein found in A. alternata that causes severe asthma. Despite the well-established pathogenicity of Alt a 1, the molecular mechanisms underlying its action and physiological function remain largely unknown. To gain insight into the role played by this protein in the pathogenicity of the fungus, we studied production of Alt a 1 and its activity in spores. We found that Alt a 1 accumulates inside spores and that its release with a ligand is pH-dependent, with optimum production in the 5.0-6.5 interval. The Alt a 1 ligand was... (More)

Spores of pathogenic fungi are virtually ubiquitous and cause human disease and severe losses in crops. The endophytic fungi Alternaria species produce host-selective phytotoxins. Alt a 1 is a strongly allergenic protein found in A. alternata that causes severe asthma. Despite the well-established pathogenicity of Alt a 1, the molecular mechanisms underlying its action and physiological function remain largely unknown. To gain insight into the role played by this protein in the pathogenicity of the fungus, we studied production of Alt a 1 and its activity in spores. We found that Alt a 1 accumulates inside spores and that its release with a ligand is pH-dependent, with optimum production in the 5.0-6.5 interval. The Alt a 1 ligand was identified as a methylated flavonoid that inhibits plant root growth and detoxifies reactive oxygen species. We also found that Alt a 1 changes its oligomerization state depending on the pH of the surrounding medium and that these changes facilitate the release of the ligand. Based on these results, we propose that release of Alt a 1 should be a pathogenic target in approaches used to block plant defenses and consequently to favor fungal entry into the plant.

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author
organization
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type
Contribution to journal
publication status
published
subject
in
Scientific Reports
volume
6
publisher
Nature Publishing Group
external identifiers
  • scopus:84988010159
  • wos:000383647100001
ISSN
2045-2322
DOI
10.1038/srep33468
language
English
LU publication?
yes
id
88e67210-6a05-4e67-afa1-03cbf39da507
date added to LUP
2016-11-03 10:40:23
date last changed
2017-01-01 08:38:22
@article{88e67210-6a05-4e67-afa1-03cbf39da507,
  abstract     = {<p>Spores of pathogenic fungi are virtually ubiquitous and cause human disease and severe losses in crops. The endophytic fungi Alternaria species produce host-selective phytotoxins. Alt a 1 is a strongly allergenic protein found in A. alternata that causes severe asthma. Despite the well-established pathogenicity of Alt a 1, the molecular mechanisms underlying its action and physiological function remain largely unknown. To gain insight into the role played by this protein in the pathogenicity of the fungus, we studied production of Alt a 1 and its activity in spores. We found that Alt a 1 accumulates inside spores and that its release with a ligand is pH-dependent, with optimum production in the 5.0-6.5 interval. The Alt a 1 ligand was identified as a methylated flavonoid that inhibits plant root growth and detoxifies reactive oxygen species. We also found that Alt a 1 changes its oligomerization state depending on the pH of the surrounding medium and that these changes facilitate the release of the ligand. Based on these results, we propose that release of Alt a 1 should be a pathogenic target in approaches used to block plant defenses and consequently to favor fungal entry into the plant.</p>},
  articleno    = {33468},
  author       = {Garrido-Arandia, Mariá and Silva-Navas, Javier and Ramírez-Castillejo, Carmen and Cubells-Baeza, Nuria and Gómez-Casado, Cristina and Barber, Domingo and Pozo, Juan C. and Melendi, Pablo G. and Pacios, Luis F. and Diáz-Perales, Araceli},
  issn         = {2045-2322},
  language     = {eng},
  month        = {09},
  publisher    = {Nature Publishing Group},
  series       = {Scientific Reports},
  title        = {Characterisation of a flavonoid ligand of the fungal protein Alt a 1},
  url          = {http://dx.doi.org/10.1038/srep33468},
  volume       = {6},
  year         = {2016},
}