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Expression of osteopontin (Eta-1) in Crohn disease of the terminal ileum

Gassler, N; Autschbach, F; Gauer, S; Bohn, Jörg LU ; Sido, B; Otto, HF; Geiger, H and Obermuller, N (2002) In Scandinavian Journal of Gastroenterology 37(11). p.1286-1295
Abstract
Background: The causes of Crohn disease (CD) are still regarded as unknown, but impaired mucosal immunoregulation with activation of T-helper-1 (Th-1) cytokine responses is probably involved and may contribute to the morphological changes. We investigated a possible role of osteopontin (Opn) in the pathogenesis of CD. This glycoprotein has been suggested to be involved in the generation of Th-1-type immune responses; moreover, it carries anti-inflammatory activities. Methods: Ileal samples from CD patients-both actively inflamed and inactive areas as well as unaffected intestinal specimens from controls (normal ileum)-were investigated by Western blot analysis, immunohistochemistry and in situ hybridization. Results: In normal gut, Opn was... (More)
Background: The causes of Crohn disease (CD) are still regarded as unknown, but impaired mucosal immunoregulation with activation of T-helper-1 (Th-1) cytokine responses is probably involved and may contribute to the morphological changes. We investigated a possible role of osteopontin (Opn) in the pathogenesis of CD. This glycoprotein has been suggested to be involved in the generation of Th-1-type immune responses; moreover, it carries anti-inflammatory activities. Methods: Ileal samples from CD patients-both actively inflamed and inactive areas as well as unaffected intestinal specimens from controls (normal ileum)-were investigated by Western blot analysis, immunohistochemistry and in situ hybridization. Results: In normal gut, Opn was found to be regularly expressed by plasma cells (CD 38) and a subset of lamina propria mononuclear cells (MNC) as well as by intestinal epithelial cells (IEC). In active CD, immunohistochemistry and in situ hybridization analysis revealed a loss of Opn expression by IEC adjacent to ulcerative lesions, whereas especially plasma cells (CD 38) in the vicinity of such lesions were found to express the molecule. In addition, a slight overexpression of Opn protein was found in metaplastic crypts. However, quantitative analysis of total Opn protein in the ileal mucosa of CD patients did not reveal any difference vis-A-vis control tissues. Conclusions: The constitutive expression of Opn in normal gut indicates that it is involved in intestinal immune homeostasis. Downregulation of Opn expression in IEC might favour the disintegration of the epithelial barrier. The expression of Opn in lamina propria plasma cells could contribute to disease chronification, probably by affecting cell survival. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
ileum, Crohn disease, enterocytes, osteopontin
in
Scandinavian Journal of Gastroenterology
volume
37
issue
11
pages
1286 - 1295
publisher
Taylor & Francis
external identifiers
  • wos:000179402000009
  • pmid:12465727
  • scopus:0036844939
ISSN
1502-7708
language
English
LU publication?
yes
id
0042b406-abd7-481a-ada8-5b0230cf2e3b (old id 892285)
alternative location
http://www.ingentaconnect.com/content/apl/sgas/2002/00000037/00000011/art00009
date added to LUP
2008-01-22 09:09:31
date last changed
2017-08-13 04:30:51
@article{0042b406-abd7-481a-ada8-5b0230cf2e3b,
  abstract     = {Background: The causes of Crohn disease (CD) are still regarded as unknown, but impaired mucosal immunoregulation with activation of T-helper-1 (Th-1) cytokine responses is probably involved and may contribute to the morphological changes. We investigated a possible role of osteopontin (Opn) in the pathogenesis of CD. This glycoprotein has been suggested to be involved in the generation of Th-1-type immune responses; moreover, it carries anti-inflammatory activities. Methods: Ileal samples from CD patients-both actively inflamed and inactive areas as well as unaffected intestinal specimens from controls (normal ileum)-were investigated by Western blot analysis, immunohistochemistry and in situ hybridization. Results: In normal gut, Opn was found to be regularly expressed by plasma cells (CD 38) and a subset of lamina propria mononuclear cells (MNC) as well as by intestinal epithelial cells (IEC). In active CD, immunohistochemistry and in situ hybridization analysis revealed a loss of Opn expression by IEC adjacent to ulcerative lesions, whereas especially plasma cells (CD 38) in the vicinity of such lesions were found to express the molecule. In addition, a slight overexpression of Opn protein was found in metaplastic crypts. However, quantitative analysis of total Opn protein in the ileal mucosa of CD patients did not reveal any difference vis-A-vis control tissues. Conclusions: The constitutive expression of Opn in normal gut indicates that it is involved in intestinal immune homeostasis. Downregulation of Opn expression in IEC might favour the disintegration of the epithelial barrier. The expression of Opn in lamina propria plasma cells could contribute to disease chronification, probably by affecting cell survival.},
  author       = {Gassler, N and Autschbach, F and Gauer, S and Bohn, Jörg and Sido, B and Otto, HF and Geiger, H and Obermuller, N},
  issn         = {1502-7708},
  keyword      = {ileum,Crohn disease,enterocytes,osteopontin},
  language     = {eng},
  number       = {11},
  pages        = {1286--1295},
  publisher    = {Taylor & Francis},
  series       = {Scandinavian Journal of Gastroenterology},
  title        = {Expression of osteopontin (Eta-1) in Crohn disease of the terminal ileum},
  volume       = {37},
  year         = {2002},
}