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Dosage considerations in patch testing with liquid allergens

Shaw, DW; Zhai, HB; Maibach, HI and Niklasson, Bo LU (2002) In Contact Dermatitis 47(2). p.86-90
Abstract
This study examines reproducibility of water and ethanol drop volumes from plastic squeeze dropper bottles, examines the difference in drop volumes between commonly used liquid patch test solutions, and evaluates the volumes of water and ethanol needed to saturate Finn and IQ Chamber filter papers. 2 plastic squeeze dropper bottles recommended for use in patch testing have poor reproducibility compared to other bottles tested. 3 aqueous allergens tested (formaldehyde 1%, methylchloroisothiazolinone/methylisothiazolinone 0.01%, and dimethylol dihydroxyethyleneurea 4.5%) have drop volumes equivalent to water. Smaller drop volumes are produced by ethanol, hydrocortisone butyrate 1% in ethanol, cocamidopropyl betaine 1% aq., and propylene... (More)
This study examines reproducibility of water and ethanol drop volumes from plastic squeeze dropper bottles, examines the difference in drop volumes between commonly used liquid patch test solutions, and evaluates the volumes of water and ethanol needed to saturate Finn and IQ Chamber filter papers. 2 plastic squeeze dropper bottles recommended for use in patch testing have poor reproducibility compared to other bottles tested. 3 aqueous allergens tested (formaldehyde 1%, methylchloroisothiazolinone/methylisothiazolinone 0.01%, and dimethylol dihydroxyethyleneurea 4.5%) have drop volumes equivalent to water. Smaller drop volumes are produced by ethanol, hydrocortisone butyrate 1% in ethanol, cocamidopropyl betaine 1% aq., and propylene glycol 30% aq. Filter paper saturation volumes using distilled water are 16-19 muL in standard Finn Chambers and 29-35 muL in IQ Chambers. Ethanol saturation volumes are slightly lower. Previously recommended volumes of application for aqueous allergens of 15 muL for standard Finn Chambers and 25 muL for IQ Chambers (slightly below the filter paper saturation points) are appropriate. Selection of dropper bottles should consider drop volume reproducibility, differing drop volumes for different allergens, and the patch test chamber system being used. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
standards), methods, patch tests (instrumentation, reproducibility of, results
in
Contact Dermatitis
volume
47
issue
2
pages
86 - 90
publisher
Federation of European Neuroscience Societies and Blackwell Publishing Ltd
external identifiers
  • wos:000179138600005
  • pmid:12423405
  • scopus:0036702690
ISSN
0105-1873
DOI
10.1034/j.1600-0536.2002.470205.x
language
English
LU publication?
yes
id
b254fe5d-2ea5-4cf0-952c-8ad0b6da572d (old id 892495)
date added to LUP
2008-01-21 09:18:33
date last changed
2017-10-01 03:44:11
@article{b254fe5d-2ea5-4cf0-952c-8ad0b6da572d,
  abstract     = {This study examines reproducibility of water and ethanol drop volumes from plastic squeeze dropper bottles, examines the difference in drop volumes between commonly used liquid patch test solutions, and evaluates the volumes of water and ethanol needed to saturate Finn and IQ Chamber filter papers. 2 plastic squeeze dropper bottles recommended for use in patch testing have poor reproducibility compared to other bottles tested. 3 aqueous allergens tested (formaldehyde 1%, methylchloroisothiazolinone/methylisothiazolinone 0.01%, and dimethylol dihydroxyethyleneurea 4.5%) have drop volumes equivalent to water. Smaller drop volumes are produced by ethanol, hydrocortisone butyrate 1% in ethanol, cocamidopropyl betaine 1% aq., and propylene glycol 30% aq. Filter paper saturation volumes using distilled water are 16-19 muL in standard Finn Chambers and 29-35 muL in IQ Chambers. Ethanol saturation volumes are slightly lower. Previously recommended volumes of application for aqueous allergens of 15 muL for standard Finn Chambers and 25 muL for IQ Chambers (slightly below the filter paper saturation points) are appropriate. Selection of dropper bottles should consider drop volume reproducibility, differing drop volumes for different allergens, and the patch test chamber system being used.},
  author       = {Shaw, DW and Zhai, HB and Maibach, HI and Niklasson, Bo},
  issn         = {0105-1873},
  keyword      = {standards),methods,patch tests (instrumentation,reproducibility of,results},
  language     = {eng},
  number       = {2},
  pages        = {86--90},
  publisher    = {Federation of European Neuroscience Societies and Blackwell Publishing Ltd},
  series       = {Contact Dermatitis},
  title        = {Dosage considerations in patch testing with liquid allergens},
  url          = {http://dx.doi.org/10.1034/j.1600-0536.2002.470205.x},
  volume       = {47},
  year         = {2002},
}