Effects of topping and non-topping on growth-regulating hormones of flue-cured tobacco (Nicotiana tabacum L.)—a proteomic analysis
Gu, Kaiyuan ; Yang, Li E. ; Ren, Ke ; Luo, Xianxue ; Qin, Xiao ; Op de Beeck, Michiel LU
; He, Conglian
; Jian, Li
and Chen, Yi
(2023)
In Frontiers in Plant Science
14.
- Abstract
Introduction: Until now, the mechanism underlying the impact of topping on hormone regulation in tobacco plants remains unclear, and most studies investigating the hormone signaling pathways in plants rely on genes or transcriptional pathways. Methods: This study examines the regulatory mechanisms of hormones in the roots and leaves of tobacco plants with and without topping at the protein level. Results: The results demonstrate that, compared with non-topped plants, topping leads to a decrease in the levels of IAA (auxin), ABA (abscisic acid), and GA (gibberellin) hormones in the leaves, whereas the content of the JA (jasmonic acid) hormone increases. Furthermore, in the roots, topping results in an increase in the levels of IAA, ABA,... (More)
Introduction: Until now, the mechanism underlying the impact of topping on hormone regulation in tobacco plants remains unclear, and most studies investigating the hormone signaling pathways in plants rely on genes or transcriptional pathways. Methods: This study examines the regulatory mechanisms of hormones in the roots and leaves of tobacco plants with and without topping at the protein level. Results: The results demonstrate that, compared with non-topped plants, topping leads to a decrease in the levels of IAA (auxin), ABA (abscisic acid), and GA (gibberellin) hormones in the leaves, whereas the content of the JA (jasmonic acid) hormone increases. Furthermore, in the roots, topping results in an increase in the levels of IAA, ABA, and JA hormones, along with a decrease in GA content. In the leaves, a total of 258 significantly different proteins were identified before and after topping, with 128 proteins upregulated and 130 proteins downregulated. In the roots, there were 439 proteins with significantly different quantities before and after topping, consisting of 211 upregulated proteins and 228 downregulated proteins. Notably, these proteins were closely associated with the metabolic and biosynthetic pathways of secondary metabolites, as indicated by functional categorization. Conclusions: When integrating the hormone changes and the proteomics results, it is evident that topping leads to increased metabolic activity and enhanced hormone synthesis in the root system. This research provides a theoretical foundation for further investigations into the regulation and signaling mechanisms of hormones at the protein level before and after topping in plants.
(Less)
- author
- Gu, Kaiyuan
; Yang, Li E.
; Ren, Ke
; Luo, Xianxue
; Qin, Xiao
; Op de Beeck, Michiel
LU
; He, Conglian
; Jian, Li
and Chen, Yi
- organization
- publishing date
- 2023-10-30
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- leaves and roots, plant hormones, proteomics, tobacco, topping
- in
- Frontiers in Plant Science
- volume
- 14
- article number
- 1255252
- pages
- 12 pages
- publisher
- Frontiers Media S. A.
- external identifiers
-
- pmid:38023860
- scopus:85176402092
- ISSN
- 1664-462X
- DOI
- 10.3389/fpls.2023.1255252
- language
- English
- LU publication?
- yes
- id
- 892adae6-ec96-4c22-9762-c048ee8f83ed
- date added to LUP
- 2024-01-12 10:16:54
- date last changed
- 2024-04-27 05:42:22
@article{892adae6-ec96-4c22-9762-c048ee8f83ed,
abstract = {{<p>Introduction: Until now, the mechanism underlying the impact of topping on hormone regulation in tobacco plants remains unclear, and most studies investigating the hormone signaling pathways in plants rely on genes or transcriptional pathways. Methods: This study examines the regulatory mechanisms of hormones in the roots and leaves of tobacco plants with and without topping at the protein level. Results: The results demonstrate that, compared with non-topped plants, topping leads to a decrease in the levels of IAA (auxin), ABA (abscisic acid), and GA (gibberellin) hormones in the leaves, whereas the content of the JA (jasmonic acid) hormone increases. Furthermore, in the roots, topping results in an increase in the levels of IAA, ABA, and JA hormones, along with a decrease in GA content. In the leaves, a total of 258 significantly different proteins were identified before and after topping, with 128 proteins upregulated and 130 proteins downregulated. In the roots, there were 439 proteins with significantly different quantities before and after topping, consisting of 211 upregulated proteins and 228 downregulated proteins. Notably, these proteins were closely associated with the metabolic and biosynthetic pathways of secondary metabolites, as indicated by functional categorization. Conclusions: When integrating the hormone changes and the proteomics results, it is evident that topping leads to increased metabolic activity and enhanced hormone synthesis in the root system. This research provides a theoretical foundation for further investigations into the regulation and signaling mechanisms of hormones at the protein level before and after topping in plants.</p>}},
author = {{Gu, Kaiyuan and Yang, Li E. and Ren, Ke and Luo, Xianxue and Qin, Xiao and Op de Beeck, Michiel and He, Conglian and Jian, Li and Chen, Yi}},
issn = {{1664-462X}},
keywords = {{leaves and roots; plant hormones; proteomics; tobacco; topping}},
language = {{eng}},
month = {{10}},
publisher = {{Frontiers Media S. A.}},
series = {{Frontiers in Plant Science}},
title = {{Effects of topping and non-topping on growth-regulating hormones of flue-cured tobacco (Nicotiana tabacum L.)—a proteomic analysis}},
url = {{http://dx.doi.org/10.3389/fpls.2023.1255252}},
doi = {{10.3389/fpls.2023.1255252}},
volume = {{14}},
year = {{2023}},
}