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Cellular and subcellular localization of an M(r) 64,000 protein autoantigen in insulin-dependent diabetes

Christie, M. R. ; Pipeleers, D. G. ; Lernmark, A. LU orcid and Baekkeskov, S. (1990) In Journal of Biological Chemistry 265(1). p.376-381
Abstract

Antibodies to an M(r) 64,000 protein from human or rat islets have been detected at high frequency in newly diagnosed insulin-dependent diabetic patients. In this study, we show that the antigenic and amphiphilic properties of the rat islet M(r) 64,000 protein resemble that of the human protein. We have analyzed the expression of the M(r) 64,000 protein in populations of pancreatic β and non-β cells and in selected rat tissues by immunoprecipitation of [35S]methionine-radiolabeled proteins with sera from diabetic patients or from healthy control individuals. When islet cell populations enriched in β or non-β cells were tested for the expression of the M(r) 64,000 antigen, the protein was primarily observed in the β cells. On... (More)

Antibodies to an M(r) 64,000 protein from human or rat islets have been detected at high frequency in newly diagnosed insulin-dependent diabetic patients. In this study, we show that the antigenic and amphiphilic properties of the rat islet M(r) 64,000 protein resemble that of the human protein. We have analyzed the expression of the M(r) 64,000 protein in populations of pancreatic β and non-β cells and in selected rat tissues by immunoprecipitation of [35S]methionine-radiolabeled proteins with sera from diabetic patients or from healthy control individuals. When islet cell populations enriched in β or non-β cells were tested for the expression of the M(r) 64,000 antigen, the protein was primarily observed in the β cells. On analyzing preparations of islets, liver, kidney, thyroid, adrenal, pituitary, spleen, and thymus, the protein could only be detected in islets. The protein was also characterized in terms of its subcellular localization by Percoll density gradient centrifugation and was recovered in a fraction enriched in the plasma membrane marker, 5'-nucleotidase. These results are consistent with a β cell-restricted plasma membrane expression of the protein and support the hypothesis that this protein is a target antigen of β cell-specific autoimmunity in insulin-dependent diabetes.

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author
; ; and
publishing date
type
Contribution to journal
publication status
published
in
Journal of Biological Chemistry
volume
265
issue
1
pages
6 pages
publisher
American Society for Biochemistry and Molecular Biology
external identifiers
  • scopus:0025191302
  • pmid:2403561
ISSN
0021-9258
language
English
LU publication?
no
id
89763ec0-3779-4675-8f2e-6873d7c543f4
date added to LUP
2019-09-11 09:54:37
date last changed
2024-03-13 07:59:16
@article{89763ec0-3779-4675-8f2e-6873d7c543f4,
  abstract     = {{<p>Antibodies to an M(r) 64,000 protein from human or rat islets have been detected at high frequency in newly diagnosed insulin-dependent diabetic patients. In this study, we show that the antigenic and amphiphilic properties of the rat islet M(r) 64,000 protein resemble that of the human protein. We have analyzed the expression of the M(r) 64,000 protein in populations of pancreatic β and non-β cells and in selected rat tissues by immunoprecipitation of [<sup>35</sup>S]methionine-radiolabeled proteins with sera from diabetic patients or from healthy control individuals. When islet cell populations enriched in β or non-β cells were tested for the expression of the M(r) 64,000 antigen, the protein was primarily observed in the β cells. On analyzing preparations of islets, liver, kidney, thyroid, adrenal, pituitary, spleen, and thymus, the protein could only be detected in islets. The protein was also characterized in terms of its subcellular localization by Percoll density gradient centrifugation and was recovered in a fraction enriched in the plasma membrane marker, 5'-nucleotidase. These results are consistent with a β cell-restricted plasma membrane expression of the protein and support the hypothesis that this protein is a target antigen of β cell-specific autoimmunity in insulin-dependent diabetes.</p>}},
  author       = {{Christie, M. R. and Pipeleers, D. G. and Lernmark, A. and Baekkeskov, S.}},
  issn         = {{0021-9258}},
  language     = {{eng}},
  month        = {{01}},
  number       = {{1}},
  pages        = {{376--381}},
  publisher    = {{American Society for Biochemistry and Molecular Biology}},
  series       = {{Journal of Biological Chemistry}},
  title        = {{Cellular and subcellular localization of an M(r) 64,000 protein autoantigen in insulin-dependent diabetes}},
  volume       = {{265}},
  year         = {{1990}},
}