Retinal differentiation in an altricial bird species, Taeniopygia guttata : An immunohistochemical study
(2020) In Experimental Eye Research 190.- Abstract
The bird retina offers an excellent model to investigate the mechanisms that coordinate the morphogenesis, histogenesis, and differentiation of neuron and glial cells. Although these developmental features have been intensively studied in the chicken (Gallus gallus, Linnaeus 1758), a precocial bird species, little is known about retinogenesis in altricial birds. The purpose of this study was to examine the differentiation of retinal cells in the altricial zebra finch (Taeniopygia guttata, Vieillot, 1817) and compare the results with those from previous studies in G. gallus. By using immunohistochemical techniques, the first differentiated TUJ1-/Isl1-positive neuroblasts were detected in the vitreal surface of the neuroblastic layer at... (More)
The bird retina offers an excellent model to investigate the mechanisms that coordinate the morphogenesis, histogenesis, and differentiation of neuron and glial cells. Although these developmental features have been intensively studied in the chicken (Gallus gallus, Linnaeus 1758), a precocial bird species, little is known about retinogenesis in altricial birds. The purpose of this study was to examine the differentiation of retinal cells in the altricial zebra finch (Taeniopygia guttata, Vieillot, 1817) and compare the results with those from previous studies in G. gallus. By using immunohistochemical techniques, the first differentiated TUJ1-/Isl1-positive neuroblasts were detected in the vitreal surface of the neuroblastic layer at later incubation times in T. guttata than in G. gallus (108 h vs 55 h). The immunoreactivity of these early differentiation markers coincided temporo-spatially with the appearance of the first PCNA-negative nuclei. Furthermore, the first visinin-positive photoreceptors (132 h vs 120 h) and the first Prox-1-immunoreactive neuroblasts (embryonic day 7.25 (E7.25) vs E6.5) were also detected at later embryonic stages in the retina of T. guttata than in the retina of G. gallus. At E13, one day before hatching, abundant PCNA- and pHisH3-immunoreactivities were detected in the T. guttata retina, while proliferation was almost absent in the G. gallus retina at perinatal stages. Therefore, these results suggest that cell differentiation in the retina is delayed in the altricial bird compared to precocial birds. Furthermore, the T. guttata retina was not completely developed at hatching, and abundant mitotically active precursor cells of retinal neurons were found, suggesting that retinal neurogenesis was intense at perinatal stages.
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- author
- Álvarez-Hernán, Guadalupe LU ; Hernández-Núñez, Ismael ; Rico-Leo, Eva María ; Marzal, Alfonso LU ; de Mera-Rodríguez, José Antonio ; Rodríguez-León, Joaquín ; Martín-Partido, Gervasio and Francisco-Morcillo, Javier
- publishing date
- 2020-01
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Animals, Animals, Newborn, Biomarkers/metabolism, Blotting, Western, Cell Differentiation/physiology, Cell Proliferation/physiology, Chick Embryo, Embryonic Development/physiology, Eye Proteins/metabolism, Finches/embryology, Immunohistochemistry, Neurogenesis/physiology, Retina/cytology
- in
- Experimental Eye Research
- volume
- 190
- article number
- 107869
- publisher
- Elsevier
- external identifiers
-
- pmid:31705900
- scopus:85074891903
- ISSN
- 0014-4835
- DOI
- 10.1016/j.exer.2019.107869
- language
- English
- LU publication?
- no
- additional info
- Copyright © 2019 Elsevier Ltd. All rights reserved.
- id
- 8980815d-5c88-4339-8892-fcf17e997eb8
- date added to LUP
- 2021-12-15 14:06:15
- date last changed
- 2024-06-16 03:08:50
@article{8980815d-5c88-4339-8892-fcf17e997eb8,
abstract = {{<p>The bird retina offers an excellent model to investigate the mechanisms that coordinate the morphogenesis, histogenesis, and differentiation of neuron and glial cells. Although these developmental features have been intensively studied in the chicken (Gallus gallus, Linnaeus 1758), a precocial bird species, little is known about retinogenesis in altricial birds. The purpose of this study was to examine the differentiation of retinal cells in the altricial zebra finch (Taeniopygia guttata, Vieillot, 1817) and compare the results with those from previous studies in G. gallus. By using immunohistochemical techniques, the first differentiated TUJ1-/Isl1-positive neuroblasts were detected in the vitreal surface of the neuroblastic layer at later incubation times in T. guttata than in G. gallus (108 h vs 55 h). The immunoreactivity of these early differentiation markers coincided temporo-spatially with the appearance of the first PCNA-negative nuclei. Furthermore, the first visinin-positive photoreceptors (132 h vs 120 h) and the first Prox-1-immunoreactive neuroblasts (embryonic day 7.25 (E7.25) vs E6.5) were also detected at later embryonic stages in the retina of T. guttata than in the retina of G. gallus. At E13, one day before hatching, abundant PCNA- and pHisH3-immunoreactivities were detected in the T. guttata retina, while proliferation was almost absent in the G. gallus retina at perinatal stages. Therefore, these results suggest that cell differentiation in the retina is delayed in the altricial bird compared to precocial birds. Furthermore, the T. guttata retina was not completely developed at hatching, and abundant mitotically active precursor cells of retinal neurons were found, suggesting that retinal neurogenesis was intense at perinatal stages.</p>}},
author = {{Álvarez-Hernán, Guadalupe and Hernández-Núñez, Ismael and Rico-Leo, Eva María and Marzal, Alfonso and de Mera-Rodríguez, José Antonio and Rodríguez-León, Joaquín and Martín-Partido, Gervasio and Francisco-Morcillo, Javier}},
issn = {{0014-4835}},
keywords = {{Animals; Animals, Newborn; Biomarkers/metabolism; Blotting, Western; Cell Differentiation/physiology; Cell Proliferation/physiology; Chick Embryo; Embryonic Development/physiology; Eye Proteins/metabolism; Finches/embryology; Immunohistochemistry; Neurogenesis/physiology; Retina/cytology}},
language = {{eng}},
publisher = {{Elsevier}},
series = {{Experimental Eye Research}},
title = {{Retinal differentiation in an altricial bird species, Taeniopygia guttata : An immunohistochemical study}},
url = {{http://dx.doi.org/10.1016/j.exer.2019.107869}},
doi = {{10.1016/j.exer.2019.107869}},
volume = {{190}},
year = {{2020}},
}