Electrocatalytic oxidation of NADH using a pencil graphite electrode modified with quercetin
(2013) In Colloids and Surfaces B: Biointerfaces 102. p.816-821- Abstract
- In the present study, the electrocatalytic oxidation of reduced beta nicotinamide adenine dinucleotide (NADH) was investigated using a pencil graphite electrode modified with quercetin (PGE/QH(2)). The PGE/QH(2) was prepared through two steps: (i) the pre-treatment of PGE at 1.40V vs. Ag vertical bar AgCl vertical bar KCl(sat.) in pH 7.0 phosphate buffer containing 0.1 M KCl for 60 s and (ii) adsorption of QH(2) on the PGE via immersion of PGE into a 1.0 mM QH(2) solution (in ethanol) for 60 s. Cyclic voltammetric studies show that the peak potential of NADH oxidation shifts from +500 mV at bare PGE to +300 mV at PGE/QH(2). The electrocatalytic currents obtained from amperometric measurements at +300 mV vs. Ag vertical bar AgCl vertical... (More)
- In the present study, the electrocatalytic oxidation of reduced beta nicotinamide adenine dinucleotide (NADH) was investigated using a pencil graphite electrode modified with quercetin (PGE/QH(2)). The PGE/QH(2) was prepared through two steps: (i) the pre-treatment of PGE at 1.40V vs. Ag vertical bar AgCl vertical bar KCl(sat.) in pH 7.0 phosphate buffer containing 0.1 M KCl for 60 s and (ii) adsorption of QH(2) on the PGE via immersion of PGE into a 1.0 mM QH(2) solution (in ethanol) for 60 s. Cyclic voltammetric studies show that the peak potential of NADH oxidation shifts from +500 mV at bare PGE to +300 mV at PGE/QH(2). The electrocatalytic currents obtained from amperometric measurements at +300 mV vs. Ag vertical bar AgCl vertical bar KCl(sat.) and in phosphate buffer solution at pH 7.0 containing 0.1 M KCl were linearly related to the concentration of NADH. Linear calibration plots are obtained in the concentration range from 0.5 mu M to 100 mu M. The limit of detection was found to be 0.15 mu M. Crown Copyright (c) 2012 Published by Elsevier B.V. All rights reserved. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/3590197
- author
- Dilgin, Yusuf ; Kizilkaya, Bayram ; Dilgin, Didem Giray ; Gokcel, H. Ismet and Gorton, Lo LU
- organization
- publishing date
- 2013
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Electrocatalytic oxidation, Modified electrode, Quercetin, NADH, Pencil, graphite electrode
- in
- Colloids and Surfaces B: Biointerfaces
- volume
- 102
- pages
- 816 - 821
- publisher
- Elsevier
- external identifiers
-
- wos:000313301200116
- scopus:84867772909
- pmid:23107961
- ISSN
- 1873-4367
- DOI
- 10.1016/j.colsurfb.2012.09.030
- language
- English
- LU publication?
- yes
- id
- 898f64ee-6b0a-400b-a551-34da3ef16189 (old id 3590197)
- date added to LUP
- 2016-04-01 13:44:51
- date last changed
- 2023-11-12 21:16:01
@article{898f64ee-6b0a-400b-a551-34da3ef16189,
abstract = {{In the present study, the electrocatalytic oxidation of reduced beta nicotinamide adenine dinucleotide (NADH) was investigated using a pencil graphite electrode modified with quercetin (PGE/QH(2)). The PGE/QH(2) was prepared through two steps: (i) the pre-treatment of PGE at 1.40V vs. Ag vertical bar AgCl vertical bar KCl(sat.) in pH 7.0 phosphate buffer containing 0.1 M KCl for 60 s and (ii) adsorption of QH(2) on the PGE via immersion of PGE into a 1.0 mM QH(2) solution (in ethanol) for 60 s. Cyclic voltammetric studies show that the peak potential of NADH oxidation shifts from +500 mV at bare PGE to +300 mV at PGE/QH(2). The electrocatalytic currents obtained from amperometric measurements at +300 mV vs. Ag vertical bar AgCl vertical bar KCl(sat.) and in phosphate buffer solution at pH 7.0 containing 0.1 M KCl were linearly related to the concentration of NADH. Linear calibration plots are obtained in the concentration range from 0.5 mu M to 100 mu M. The limit of detection was found to be 0.15 mu M. Crown Copyright (c) 2012 Published by Elsevier B.V. All rights reserved.}},
author = {{Dilgin, Yusuf and Kizilkaya, Bayram and Dilgin, Didem Giray and Gokcel, H. Ismet and Gorton, Lo}},
issn = {{1873-4367}},
keywords = {{Electrocatalytic oxidation; Modified electrode; Quercetin; NADH; Pencil; graphite electrode}},
language = {{eng}},
pages = {{816--821}},
publisher = {{Elsevier}},
series = {{Colloids and Surfaces B: Biointerfaces}},
title = {{Electrocatalytic oxidation of NADH using a pencil graphite electrode modified with quercetin}},
url = {{http://dx.doi.org/10.1016/j.colsurfb.2012.09.030}},
doi = {{10.1016/j.colsurfb.2012.09.030}},
volume = {{102}},
year = {{2013}},
}