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Immune response to Mycoplasma pneumoniae P1 and P116 in patients with atypical pneumonia analyzed by ELISA

Drasbek, M; Nielsen, PK; Persson, Kenneth LU ; Birkelund, S and Christiansen, G (2004) In BMC Microbiology 4.
Abstract
Background: Serology is often used for the diagnosis of Mycoplasma pneumoniae. It is important to identify specific antigens that can distinguish between the presence or absence of antibodies against M. pneumoniae. The two proteins, P116 and P1, are found to be immunogenic. By using these in ELISA it is possible to identify an immune response against M. pneumoniae in serum samples. Results: A recombinant protein derived from the P116 protein and one from the P1 protein were used in two ELISA tests, rP116-ELISA and rP1-ELISA. Human serum samples from patients with atypical pneumonia were tested and compared to the results of the complement fixation test. There was a good agreement between the two tests but the rP1-ELISA showed the best... (More)
Background: Serology is often used for the diagnosis of Mycoplasma pneumoniae. It is important to identify specific antigens that can distinguish between the presence or absence of antibodies against M. pneumoniae. The two proteins, P116 and P1, are found to be immunogenic. By using these in ELISA it is possible to identify an immune response against M. pneumoniae in serum samples. Results: A recombinant protein derived from the P116 protein and one from the P1 protein were used in two ELISA tests, rP116-ELISA and rP1-ELISA. Human serum samples from patients with atypical pneumonia were tested and compared to the results of the complement fixation test. There was a good agreement between the two tests but the rP1-ELISA showed the best discrimination between positive and negative samples. Conclusion: Two ELISA tests based on recombinant proteins have been analysed and compared to the complement fixation test results. The two ELISA tests were found suitable for use in serodiagnostics of M. pneumoniae infections. The use of specific antigens eliminates the risk of cross reaction to an immune response against other bacteria. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
BMC Microbiology
volume
4
publisher
BioMed Central
external identifiers
  • wos:000220145000001
  • pmid:15018643
  • scopus:2642514901
ISSN
1471-2180
DOI
10.1186/1471-2180-4-7
language
English
LU publication?
yes
id
82b93693-51d9-4a7f-bc1a-43f55ac211df (old id 899277)
date added to LUP
2008-01-10 14:00:12
date last changed
2017-07-02 04:20:35
@article{82b93693-51d9-4a7f-bc1a-43f55ac211df,
  abstract     = {Background: Serology is often used for the diagnosis of Mycoplasma pneumoniae. It is important to identify specific antigens that can distinguish between the presence or absence of antibodies against M. pneumoniae. The two proteins, P116 and P1, are found to be immunogenic. By using these in ELISA it is possible to identify an immune response against M. pneumoniae in serum samples. Results: A recombinant protein derived from the P116 protein and one from the P1 protein were used in two ELISA tests, rP116-ELISA and rP1-ELISA. Human serum samples from patients with atypical pneumonia were tested and compared to the results of the complement fixation test. There was a good agreement between the two tests but the rP1-ELISA showed the best discrimination between positive and negative samples. Conclusion: Two ELISA tests based on recombinant proteins have been analysed and compared to the complement fixation test results. The two ELISA tests were found suitable for use in serodiagnostics of M. pneumoniae infections. The use of specific antigens eliminates the risk of cross reaction to an immune response against other bacteria.},
  author       = {Drasbek, M and Nielsen, PK and Persson, Kenneth and Birkelund, S and Christiansen, G},
  issn         = {1471-2180},
  language     = {eng},
  publisher    = {BioMed Central},
  series       = {BMC Microbiology},
  title        = {Immune response to Mycoplasma pneumoniae P1 and P116 in patients with atypical pneumonia analyzed by ELISA},
  url          = {http://dx.doi.org/10.1186/1471-2180-4-7},
  volume       = {4},
  year         = {2004},
}