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Effects of a topical enamel matrix derivative on skin wound healing

Mirastschijski, U; Konrad, D; Lundberg, E; Lyngstadaas, SP; Jorgensen, LN and Agren, MS (2004) In Wound Repair and Regeneration 12(1). p.100-108
Abstract
Enamel matrix derivative, obtained from developing porcine teeth, is composed mainly of amelogenin proteins and used topically in periodontal surgery for advanced periodontitis to regenerate lost connective tissues. The primary objective of this study was to investigate the effects of enamel matrix derivative on skin wound healing. Secondly, in vitro effects of enamel matrix derivative on dermal fibroblasts and microvascular endothelial cells were examined. Full-thickness, circular 2-cm skin wounds in white 16-week-old rabbits were treated thrice weekly with enamel matrix derivative (30 mg/ml) in the vehicle propylene glycol alginate or with vehicle alone. Enamel matrix derivative treatment increased the amount of granulation tissue and... (More)
Enamel matrix derivative, obtained from developing porcine teeth, is composed mainly of amelogenin proteins and used topically in periodontal surgery for advanced periodontitis to regenerate lost connective tissues. The primary objective of this study was to investigate the effects of enamel matrix derivative on skin wound healing. Secondly, in vitro effects of enamel matrix derivative on dermal fibroblasts and microvascular endothelial cells were examined. Full-thickness, circular 2-cm skin wounds in white 16-week-old rabbits were treated thrice weekly with enamel matrix derivative (30 mg/ml) in the vehicle propylene glycol alginate or with vehicle alone. Enamel matrix derivative treatment increased the amount of granulation tissue and accelerated time to complete epithelialization by 3 days (p < 0.001) compared to vehicle treatment. In cultured fibroblasts, vascular endothelial growth factor levels in conditioned media were increased more than fivefold (p < 0.001) with enamel matrix derivative treatment (0.1 mg/ml) over control, measured by specific enzyme-linked immunosorbent assay. Enamel matrix derivative also increased release of matrix metalloproteinase-2 more than threefold from fibroblasts (p < 0.001) and from endothelial cells (p < 0.001). Thus, enamel matrix derivative significantly accelerated wound closure in rabbits, possibly by increasing levels of growth factors and proteinases important for granulation tissue formation and remodeling. (Less)
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author
publishing date
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published
subject
in
Wound Repair and Regeneration
volume
12
issue
1
pages
100 - 108
publisher
Wiley-Blackwell
external identifiers
  • wos:000189143300015
  • scopus:1342281770
ISSN
1524-475X
language
English
LU publication?
no
id
55d7d1c9-b9c3-4012-9747-068d1897caca (old id 899315)
alternative location
http://www.ingentaconnect.com/content/bsc/wrr/2004/00000012/00000001/art00076
date added to LUP
2008-01-15 15:13:11
date last changed
2017-01-01 07:07:15
@article{55d7d1c9-b9c3-4012-9747-068d1897caca,
  abstract     = {Enamel matrix derivative, obtained from developing porcine teeth, is composed mainly of amelogenin proteins and used topically in periodontal surgery for advanced periodontitis to regenerate lost connective tissues. The primary objective of this study was to investigate the effects of enamel matrix derivative on skin wound healing. Secondly, in vitro effects of enamel matrix derivative on dermal fibroblasts and microvascular endothelial cells were examined. Full-thickness, circular 2-cm skin wounds in white 16-week-old rabbits were treated thrice weekly with enamel matrix derivative (30 mg/ml) in the vehicle propylene glycol alginate or with vehicle alone. Enamel matrix derivative treatment increased the amount of granulation tissue and accelerated time to complete epithelialization by 3 days (p &lt; 0.001) compared to vehicle treatment. In cultured fibroblasts, vascular endothelial growth factor levels in conditioned media were increased more than fivefold (p &lt; 0.001) with enamel matrix derivative treatment (0.1 mg/ml) over control, measured by specific enzyme-linked immunosorbent assay. Enamel matrix derivative also increased release of matrix metalloproteinase-2 more than threefold from fibroblasts (p &lt; 0.001) and from endothelial cells (p &lt; 0.001). Thus, enamel matrix derivative significantly accelerated wound closure in rabbits, possibly by increasing levels of growth factors and proteinases important for granulation tissue formation and remodeling.},
  author       = {Mirastschijski, U and Konrad, D and Lundberg, E and Lyngstadaas, SP and Jorgensen, LN and Agren, MS},
  issn         = {1524-475X},
  language     = {eng},
  number       = {1},
  pages        = {100--108},
  publisher    = {Wiley-Blackwell},
  series       = {Wound Repair and Regeneration},
  title        = {Effects of a topical enamel matrix derivative on skin wound healing},
  volume       = {12},
  year         = {2004},
}