Possible toxic effects of normal and diabetic patient serum on pancreatic B-cells
(1978) In Diabetologia 14(1). p.25-31- Abstract
Serum from normal blood-donors and juvenile diabetic patients inhibited Rb+ accumulation and stimulated release of 51Cr and insulin in suspensions of dispersed pancreatic islet cells prepared from ob/ob mouse islets, which are rich in B-cells. The effects indicate the presence of a B-cytotoxic factor in human serum. Serum from mouse and fetal calf also inhibited the islet cell accumulation of Rb+. Toxicity was not suppressed by treating serum with protein A-Sepharose and did not correlate with islet cell binding of fluorescent antibodies to human immunoglobulin. Whereas all sera inhibited Rb+ accumulation, 3 of 6 diabetic patient sera, but no blood-donor serum, made the cells fluoresce on... (More)
Serum from normal blood-donors and juvenile diabetic patients inhibited Rb+ accumulation and stimulated release of 51Cr and insulin in suspensions of dispersed pancreatic islet cells prepared from ob/ob mouse islets, which are rich in B-cells. The effects indicate the presence of a B-cytotoxic factor in human serum. Serum from mouse and fetal calf also inhibited the islet cell accumulation of Rb+. Toxicity was not suppressed by treating serum with protein A-Sepharose and did not correlate with islet cell binding of fluorescent antibodies to human immunoglobulin. Whereas all sera inhibited Rb+ accumulation, 3 of 6 diabetic patient sera, but no blood-donor serum, made the cells fluoresce on exposure to the fluorescent antibodies. Supporting a dependence on complement, toxicity remained after dialysis, but was destroyed by treating serum with zymosan-A or heating at 56 ° for 30 min.
(Less)
- author
- Lernmark, A. LU ; Sehlin, J. ; Täljedal, I. B. ; Kromann, H. and Nerup, J. LU
- publishing date
- 1978-01-01
- type
- Contribution to journal
- publication status
- published
- keywords
- autoimmunity, Diabetes mellitus, insulin release, islet cell antibodies, pancreatic B-cells, rubidium accumulation, serum complement, serum cytotoxicity
- in
- Diabetologia
- volume
- 14
- issue
- 1
- pages
- 7 pages
- publisher
- Springer
- external identifiers
-
- scopus:0017803015
- pmid:342324
- ISSN
- 0012-186X
- DOI
- 10.1007/BF00429704
- language
- English
- LU publication?
- no
- id
- 89a6e71c-6f8e-4ba5-8a23-92fab962a725
- date added to LUP
- 2019-09-18 12:08:47
- date last changed
- 2024-03-13 07:59:57
@article{89a6e71c-6f8e-4ba5-8a23-92fab962a725, abstract = {{<p>Serum from normal blood-donors and juvenile diabetic patients inhibited Rb<sup>+</sup> accumulation and stimulated release of <sup>51</sup>Cr and insulin in suspensions of dispersed pancreatic islet cells prepared from ob/ob mouse islets, which are rich in B-cells. The effects indicate the presence of a B-cytotoxic factor in human serum. Serum from mouse and fetal calf also inhibited the islet cell accumulation of Rb<sup>+</sup>. Toxicity was not suppressed by treating serum with protein A-Sepharose and did not correlate with islet cell binding of fluorescent antibodies to human immunoglobulin. Whereas all sera inhibited Rb<sup>+</sup> accumulation, 3 of 6 diabetic patient sera, but no blood-donor serum, made the cells fluoresce on exposure to the fluorescent antibodies. Supporting a dependence on complement, toxicity remained after dialysis, but was destroyed by treating serum with zymosan-A or heating at 56 ° for 30 min.</p>}}, author = {{Lernmark, A. and Sehlin, J. and Täljedal, I. B. and Kromann, H. and Nerup, J.}}, issn = {{0012-186X}}, keywords = {{autoimmunity; Diabetes mellitus; insulin release; islet cell antibodies; pancreatic B-cells; rubidium accumulation; serum complement; serum cytotoxicity}}, language = {{eng}}, month = {{01}}, number = {{1}}, pages = {{25--31}}, publisher = {{Springer}}, series = {{Diabetologia}}, title = {{Possible toxic effects of normal and diabetic patient serum on pancreatic B-cells}}, url = {{http://dx.doi.org/10.1007/BF00429704}}, doi = {{10.1007/BF00429704}}, volume = {{14}}, year = {{1978}}, }