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Possible toxic effects of normal and diabetic patient serum on pancreatic B-cells

Lernmark, A. LU orcid ; Sehlin, J. ; Täljedal, I. B. ; Kromann, H. and Nerup, J. LU (1978) In Diabetologia 14(1). p.25-31
Abstract

Serum from normal blood-donors and juvenile diabetic patients inhibited Rb+ accumulation and stimulated release of 51Cr and insulin in suspensions of dispersed pancreatic islet cells prepared from ob/ob mouse islets, which are rich in B-cells. The effects indicate the presence of a B-cytotoxic factor in human serum. Serum from mouse and fetal calf also inhibited the islet cell accumulation of Rb+. Toxicity was not suppressed by treating serum with protein A-Sepharose and did not correlate with islet cell binding of fluorescent antibodies to human immunoglobulin. Whereas all sera inhibited Rb+ accumulation, 3 of 6 diabetic patient sera, but no blood-donor serum, made the cells fluoresce on... (More)

Serum from normal blood-donors and juvenile diabetic patients inhibited Rb+ accumulation and stimulated release of 51Cr and insulin in suspensions of dispersed pancreatic islet cells prepared from ob/ob mouse islets, which are rich in B-cells. The effects indicate the presence of a B-cytotoxic factor in human serum. Serum from mouse and fetal calf also inhibited the islet cell accumulation of Rb+. Toxicity was not suppressed by treating serum with protein A-Sepharose and did not correlate with islet cell binding of fluorescent antibodies to human immunoglobulin. Whereas all sera inhibited Rb+ accumulation, 3 of 6 diabetic patient sera, but no blood-donor serum, made the cells fluoresce on exposure to the fluorescent antibodies. Supporting a dependence on complement, toxicity remained after dialysis, but was destroyed by treating serum with zymosan-A or heating at 56 ° for 30 min.

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author
; ; ; and
publishing date
type
Contribution to journal
publication status
published
keywords
autoimmunity, Diabetes mellitus, insulin release, islet cell antibodies, pancreatic B-cells, rubidium accumulation, serum complement, serum cytotoxicity
in
Diabetologia
volume
14
issue
1
pages
7 pages
publisher
Springer
external identifiers
  • pmid:342324
  • scopus:0017803015
ISSN
0012-186X
DOI
10.1007/BF00429704
language
English
LU publication?
no
id
89a6e71c-6f8e-4ba5-8a23-92fab962a725
date added to LUP
2019-09-18 12:08:47
date last changed
2024-03-13 07:59:57
@article{89a6e71c-6f8e-4ba5-8a23-92fab962a725,
  abstract     = {{<p>Serum from normal blood-donors and juvenile diabetic patients inhibited Rb<sup>+</sup> accumulation and stimulated release of <sup>51</sup>Cr and insulin in suspensions of dispersed pancreatic islet cells prepared from ob/ob mouse islets, which are rich in B-cells. The effects indicate the presence of a B-cytotoxic factor in human serum. Serum from mouse and fetal calf also inhibited the islet cell accumulation of Rb<sup>+</sup>. Toxicity was not suppressed by treating serum with protein A-Sepharose and did not correlate with islet cell binding of fluorescent antibodies to human immunoglobulin. Whereas all sera inhibited Rb<sup>+</sup> accumulation, 3 of 6 diabetic patient sera, but no blood-donor serum, made the cells fluoresce on exposure to the fluorescent antibodies. Supporting a dependence on complement, toxicity remained after dialysis, but was destroyed by treating serum with zymosan-A or heating at 56 ° for 30 min.</p>}},
  author       = {{Lernmark, A. and Sehlin, J. and Täljedal, I. B. and Kromann, H. and Nerup, J.}},
  issn         = {{0012-186X}},
  keywords     = {{autoimmunity; Diabetes mellitus; insulin release; islet cell antibodies; pancreatic B-cells; rubidium accumulation; serum complement; serum cytotoxicity}},
  language     = {{eng}},
  month        = {{01}},
  number       = {{1}},
  pages        = {{25--31}},
  publisher    = {{Springer}},
  series       = {{Diabetologia}},
  title        = {{Possible toxic effects of normal and diabetic patient serum on pancreatic B-cells}},
  url          = {{http://dx.doi.org/10.1007/BF00429704}},
  doi          = {{10.1007/BF00429704}},
  volume       = {{14}},
  year         = {{1978}},
}