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Skin permeation studies of chromium species – Evaluation of a reconstructed human epidermis model

Hagvall, L. LU ; Munem, M. ; Hoang Philipsen, M. ; Dowlatshahi Pour, M. ; Hedberg, Y. and Malmberg, P. (2023) In Toxicology in Vitro 91.
Abstract

A reconstructed human epidermis (RHE) model, the EpiDerm, was investigated and compared to human skin ex vivo regarding tissue penetration and distribution of two chromium species, relevant in both occupational and general exposure in the population. Imaging mass spectrometry was used in analysis of the sectioned tissue. The RHE model gave similar results compared to human skin ex vivo for skin penetration of CrVI. However, the penetration of CrIII into the tissue of the RHE model compared to human skin ex vivo differed markedly, such that in the RHE model the CrIII species accumulated in the tissue layer corresponding to stratum corneum whereas in human skin ex vivo, the CrIII species... (More)

A reconstructed human epidermis (RHE) model, the EpiDerm, was investigated and compared to human skin ex vivo regarding tissue penetration and distribution of two chromium species, relevant in both occupational and general exposure in the population. Imaging mass spectrometry was used in analysis of the sectioned tissue. The RHE model gave similar results compared to human skin ex vivo for skin penetration of CrVI. However, the penetration of CrIII into the tissue of the RHE model compared to human skin ex vivo differed markedly, such that in the RHE model the CrIII species accumulated in the tissue layer corresponding to stratum corneum whereas in human skin ex vivo, the CrIII species penetrated evenly through the skin tissue. Further, skin lipids such as cholesterol were less abundant in the RHE model compared to the human skin tissue. Results presented here indicate that the RHE models do not possess the same fundamental properties as human skin tissue. As the RHE models appear to be able to give false negative results, experiments using RHE models for the study of skin penetration should be evaluated with caution.

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organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Toxicology in Vitro
volume
91
article number
105636
publisher
Elsevier
external identifiers
  • pmid:37380007
  • scopus:85163800075
ISSN
0887-2333
DOI
10.1016/j.tiv.2023.105636
language
English
LU publication?
yes
id
89bb8b8f-4021-4496-9c2a-263acdeb340a
date added to LUP
2023-09-04 10:46:49
date last changed
2024-07-13 10:02:08
@article{89bb8b8f-4021-4496-9c2a-263acdeb340a,
  abstract     = {{<p>A reconstructed human epidermis (RHE) model, the EpiDerm, was investigated and compared to human skin ex vivo regarding tissue penetration and distribution of two chromium species, relevant in both occupational and general exposure in the population. Imaging mass spectrometry was used in analysis of the sectioned tissue. The RHE model gave similar results compared to human skin ex vivo for skin penetration of Cr<sup>VI</sup>. However, the penetration of Cr<sup>III</sup> into the tissue of the RHE model compared to human skin ex vivo differed markedly, such that in the RHE model the Cr<sup>III</sup> species accumulated in the tissue layer corresponding to stratum corneum whereas in human skin ex vivo, the Cr<sup>III</sup> species penetrated evenly through the skin tissue. Further, skin lipids such as cholesterol were less abundant in the RHE model compared to the human skin tissue. Results presented here indicate that the RHE models do not possess the same fundamental properties as human skin tissue. As the RHE models appear to be able to give false negative results, experiments using RHE models for the study of skin penetration should be evaluated with caution.</p>}},
  author       = {{Hagvall, L. and Munem, M. and Hoang Philipsen, M. and Dowlatshahi Pour, M. and Hedberg, Y. and Malmberg, P.}},
  issn         = {{0887-2333}},
  language     = {{eng}},
  publisher    = {{Elsevier}},
  series       = {{Toxicology in Vitro}},
  title        = {{Skin permeation studies of chromium species – Evaluation of a reconstructed human epidermis model}},
  url          = {{http://dx.doi.org/10.1016/j.tiv.2023.105636}},
  doi          = {{10.1016/j.tiv.2023.105636}},
  volume       = {{91}},
  year         = {{2023}},
}