T regulatory cells and dendritic cells protect against transfusion-related acute lung injury via IL-10
(2017) In Blood 129(18). p.2557-2569- Abstract
Transfusion-related acute lung injury (TRALI) is the leading cause of transfusion-related fatalities and is characterized by acute respiratory distress following blood transfusion. Donor antibodies are frequently involved; however, the pathogenesis and protective mechanisms in the recipient are poorly understood, and specific therapies are lacking. Using newly developed murine TRALI models based on injection of anti-major histocompatibility complex class I antibodies, we found CD4+CD25+FoxP3+ T regulatory cells (Tregs) and CD11c+ dendritic cells (DCs) to be critical effectors that protect against TRALI. Treg or DC depletion in vivo resulted in aggravated antibody-mediated acute lung injury within 90 minutes with 60% mortality upon DC... (More)
Transfusion-related acute lung injury (TRALI) is the leading cause of transfusion-related fatalities and is characterized by acute respiratory distress following blood transfusion. Donor antibodies are frequently involved; however, the pathogenesis and protective mechanisms in the recipient are poorly understood, and specific therapies are lacking. Using newly developed murine TRALI models based on injection of anti-major histocompatibility complex class I antibodies, we found CD4+CD25+FoxP3+ T regulatory cells (Tregs) and CD11c+ dendritic cells (DCs) to be critical effectors that protect against TRALI. Treg or DC depletion in vivo resulted in aggravated antibody-mediated acute lung injury within 90 minutes with 60% mortality upon DC depletion. In addition, resistance to antibody-mediated TRALI was associated with increased interleukin-10 (IL-10) levels, and IL-10 levels were found to be decreased in mice suffering from TRALI. Importantly, IL-10 injection completely prevented and rescued the development of TRALI in mice and may prove to be a promising new therapeutic approach for alleviating lung injury in this serious complication of transfusion.
(Less)
- author
- organization
- publishing date
- 2017
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Acute Lung Injury, Animals, Antibodies, Dendritic Cells, Interleukin-10, Mice, Mice, Inbred BALB C, Mice, Knockout, T-Lymphocytes, Regulatory, Transfusion Reaction, Journal Article
- in
- Blood
- volume
- 129
- issue
- 18
- pages
- 13 pages
- publisher
- American Society of Hematology
- external identifiers
-
- pmid:28202460
- scopus:85020165674
- wos:000400555800015
- ISSN
- 1528-0020
- DOI
- 10.1182/blood-2016-12-758185
- language
- English
- LU publication?
- yes
- id
- 89f9ca8a-4fc5-49cf-b026-af0a680807cb
- date added to LUP
- 2017-12-21 11:32:37
- date last changed
- 2024-10-14 17:16:02
@article{89f9ca8a-4fc5-49cf-b026-af0a680807cb, abstract = {{<p>Transfusion-related acute lung injury (TRALI) is the leading cause of transfusion-related fatalities and is characterized by acute respiratory distress following blood transfusion. Donor antibodies are frequently involved; however, the pathogenesis and protective mechanisms in the recipient are poorly understood, and specific therapies are lacking. Using newly developed murine TRALI models based on injection of anti-major histocompatibility complex class I antibodies, we found CD4+CD25+FoxP3+ T regulatory cells (Tregs) and CD11c+ dendritic cells (DCs) to be critical effectors that protect against TRALI. Treg or DC depletion in vivo resulted in aggravated antibody-mediated acute lung injury within 90 minutes with 60% mortality upon DC depletion. In addition, resistance to antibody-mediated TRALI was associated with increased interleukin-10 (IL-10) levels, and IL-10 levels were found to be decreased in mice suffering from TRALI. Importantly, IL-10 injection completely prevented and rescued the development of TRALI in mice and may prove to be a promising new therapeutic approach for alleviating lung injury in this serious complication of transfusion.</p>}}, author = {{Kapur, Rick and Kim, Michael and Aslam, Rukhsana and McVey, Mark J and Tabuchi, Arata and Luo, Alice and Liu, Jonathan and Li, Yuan and Shanmugabhavananthan, Shanjeevan and Speck, Edwin R and Zufferey, Anne and Yousef, George and Zhang, Haibo and Rondina, Matthew T and Weyrich, Andrew S and Porcelijn, Leendert and Kuebler, Wolfgang M and Slutsky, Arthur S and Semple, John W}}, issn = {{1528-0020}}, keywords = {{Acute Lung Injury; Animals; Antibodies; Dendritic Cells; Interleukin-10; Mice; Mice, Inbred BALB C; Mice, Knockout; T-Lymphocytes, Regulatory; Transfusion Reaction; Journal Article}}, language = {{eng}}, number = {{18}}, pages = {{2557--2569}}, publisher = {{American Society of Hematology}}, series = {{Blood}}, title = {{T regulatory cells and dendritic cells protect against transfusion-related acute lung injury via IL-10}}, url = {{http://dx.doi.org/10.1182/blood-2016-12-758185}}, doi = {{10.1182/blood-2016-12-758185}}, volume = {{129}}, year = {{2017}}, }