Direct neuronal glucose uptake heralds activity-dependent increases in cerebral metabolism
(2015) In Nature Communications 6.- Abstract
Metabolically, the brain is a highly active organ that relies almost exclusively on glucose as its energy source. According to the astrocyte-to-neuron lactate shuttle hypothesis, glucose is taken up by astrocytes and converted to lactate, which is then oxidized by neurons. Here we show, using two-photon imaging of a near-infrared 2-deoxyglucose analogue (2DG-IR), that glucose is taken up preferentially by neurons in awake behaving mice. Anaesthesia suppressed neuronal 2DG-IR uptake and sensory stimulation was associated with a sharp increase in neuronal, but not astrocytic, 2DG-IR uptake. Moreover, hexokinase, which catalyses the first enzymatic steps in glycolysis, was highly enriched in neurons compared with astrocytes, in mouse as... (More)
Metabolically, the brain is a highly active organ that relies almost exclusively on glucose as its energy source. According to the astrocyte-to-neuron lactate shuttle hypothesis, glucose is taken up by astrocytes and converted to lactate, which is then oxidized by neurons. Here we show, using two-photon imaging of a near-infrared 2-deoxyglucose analogue (2DG-IR), that glucose is taken up preferentially by neurons in awake behaving mice. Anaesthesia suppressed neuronal 2DG-IR uptake and sensory stimulation was associated with a sharp increase in neuronal, but not astrocytic, 2DG-IR uptake. Moreover, hexokinase, which catalyses the first enzymatic steps in glycolysis, was highly enriched in neurons compared with astrocytes, in mouse as well as in human cortex. These observations suggest that brain activity and neuronal glucose metabolism are directly linked, and identiy the neuron as the principal locus of glucose uptake as visualized by functional brain imaging.
(Less)
- author
- publishing date
- 2015-04-23
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Nature Communications
- volume
- 6
- article number
- 6807
- publisher
- Nature Publishing Group
- external identifiers
-
- scopus:84928526146
- pmid:25904018
- ISSN
- 2041-1723
- DOI
- 10.1038/ncomms7807
- language
- English
- LU publication?
- no
- id
- 8a69fa20-453e-4a4d-bdf9-51ecbfdabd8c
- date added to LUP
- 2019-05-16 16:44:46
- date last changed
- 2024-07-24 16:43:41
@article{8a69fa20-453e-4a4d-bdf9-51ecbfdabd8c, abstract = {{<p>Metabolically, the brain is a highly active organ that relies almost exclusively on glucose as its energy source. According to the astrocyte-to-neuron lactate shuttle hypothesis, glucose is taken up by astrocytes and converted to lactate, which is then oxidized by neurons. Here we show, using two-photon imaging of a near-infrared 2-deoxyglucose analogue (2DG-IR), that glucose is taken up preferentially by neurons in awake behaving mice. Anaesthesia suppressed neuronal 2DG-IR uptake and sensory stimulation was associated with a sharp increase in neuronal, but not astrocytic, 2DG-IR uptake. Moreover, hexokinase, which catalyses the first enzymatic steps in glycolysis, was highly enriched in neurons compared with astrocytes, in mouse as well as in human cortex. These observations suggest that brain activity and neuronal glucose metabolism are directly linked, and identiy the neuron as the principal locus of glucose uptake as visualized by functional brain imaging.</p>}}, author = {{Lundgaard, Iben and Li, Baoman and Xie, Lulu and Kang, Hongyi and Sanggaard, Simon and Haswell, John D.R. and Sun, Wei and Goldman, Siri and Blekot, Solomiya and Nielsen, Michael and Takano, Takahiro and Deane, Rashid and Nedergaard, Maiken}}, issn = {{2041-1723}}, language = {{eng}}, month = {{04}}, publisher = {{Nature Publishing Group}}, series = {{Nature Communications}}, title = {{Direct neuronal glucose uptake heralds activity-dependent increases in cerebral metabolism}}, url = {{http://dx.doi.org/10.1038/ncomms7807}}, doi = {{10.1038/ncomms7807}}, volume = {{6}}, year = {{2015}}, }