An enzyme-linked molecularly imprinted sorbent assay
(2000) In Analyst 125(1). p.13-16- Abstract
Based on a molecularly imprinted polymer, a competitive binding assay analogous to competitive enzyme immunoassay has been developed. The assay is specific for the herbicide 2,4-dichlorophenoxyacetic acid and uses, for the first time, an enzyme-labelled conjugate as a tracer. The label tobacco peroxidase allowed for colorimetric and chemiluminescence detection. The molecularly imprinted polymer was synthesised in the form of microspheres by precipitation polymerisation. The polymer efficiently and selectively bound the analyte in aqueous solution. Calibration curves were obtained corresponding to analyte concentrations ranging from 40-600 μg mL-1 for the colorimetric assay, and from 1-200 μg mL-1 for... (More)
Based on a molecularly imprinted polymer, a competitive binding assay analogous to competitive enzyme immunoassay has been developed. The assay is specific for the herbicide 2,4-dichlorophenoxyacetic acid and uses, for the first time, an enzyme-labelled conjugate as a tracer. The label tobacco peroxidase allowed for colorimetric and chemiluminescence detection. The molecularly imprinted polymer was synthesised in the form of microspheres by precipitation polymerisation. The polymer efficiently and selectively bound the analyte in aqueous solution. Calibration curves were obtained corresponding to analyte concentrations ranging from 40-600 μg mL-1 for the colorimetric assay, and from 1-200 μg mL-1 for chemiluminescence assay.
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- author
- Surugiu, Ioana ; Ye, Lei LU ; Yilmaz, Ecevit LU ; Dzgoev, Anatoli LU ; Danielsson, Bengt LU ; Mosbach, Klaus LU and Haupt, Karsten LU
- organization
- publishing date
- 2000-01-11
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Analyst
- volume
- 125
- issue
- 1
- pages
- 4 pages
- publisher
- Royal Society of Chemistry
- external identifiers
-
- scopus:0033971170
- ISSN
- 0003-2654
- DOI
- 10.1039/a908871g
- language
- English
- LU publication?
- yes
- id
- 8a781b3e-e5b2-4e75-b4fd-dc749b56ff46
- date added to LUP
- 2019-06-18 09:03:29
- date last changed
- 2022-01-31 21:57:33
@article{8a781b3e-e5b2-4e75-b4fd-dc749b56ff46, abstract = {{<p>Based on a molecularly imprinted polymer, a competitive binding assay analogous to competitive enzyme immunoassay has been developed. The assay is specific for the herbicide 2,4-dichlorophenoxyacetic acid and uses, for the first time, an enzyme-labelled conjugate as a tracer. The label tobacco peroxidase allowed for colorimetric and chemiluminescence detection. The molecularly imprinted polymer was synthesised in the form of microspheres by precipitation polymerisation. The polymer efficiently and selectively bound the analyte in aqueous solution. Calibration curves were obtained corresponding to analyte concentrations ranging from 40-600 μg mL<sup>-1</sup> for the colorimetric assay, and from 1-200 μg mL<sup>-1</sup> for chemiluminescence assay.</p>}}, author = {{Surugiu, Ioana and Ye, Lei and Yilmaz, Ecevit and Dzgoev, Anatoli and Danielsson, Bengt and Mosbach, Klaus and Haupt, Karsten}}, issn = {{0003-2654}}, language = {{eng}}, month = {{01}}, number = {{1}}, pages = {{13--16}}, publisher = {{Royal Society of Chemistry}}, series = {{Analyst}}, title = {{An enzyme-linked molecularly imprinted sorbent assay}}, url = {{http://dx.doi.org/10.1039/a908871g}}, doi = {{10.1039/a908871g}}, volume = {{125}}, year = {{2000}}, }