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An enzyme-linked molecularly imprinted sorbent assay

Surugiu, Ioana ; Ye, Lei LU ; Yilmaz, Ecevit LU ; Dzgoev, Anatoli LU ; Danielsson, Bengt LU ; Mosbach, Klaus LU and Haupt, Karsten LU (2000) In Analyst 125(1). p.13-16
Abstract

Based on a molecularly imprinted polymer, a competitive binding assay analogous to competitive enzyme immunoassay has been developed. The assay is specific for the herbicide 2,4-dichlorophenoxyacetic acid and uses, for the first time, an enzyme-labelled conjugate as a tracer. The label tobacco peroxidase allowed for colorimetric and chemiluminescence detection. The molecularly imprinted polymer was synthesised in the form of microspheres by precipitation polymerisation. The polymer efficiently and selectively bound the analyte in aqueous solution. Calibration curves were obtained corresponding to analyte concentrations ranging from 40-600 μg mL-1 for the colorimetric assay, and from 1-200 μg mL-1 for... (More)

Based on a molecularly imprinted polymer, a competitive binding assay analogous to competitive enzyme immunoassay has been developed. The assay is specific for the herbicide 2,4-dichlorophenoxyacetic acid and uses, for the first time, an enzyme-labelled conjugate as a tracer. The label tobacco peroxidase allowed for colorimetric and chemiluminescence detection. The molecularly imprinted polymer was synthesised in the form of microspheres by precipitation polymerisation. The polymer efficiently and selectively bound the analyte in aqueous solution. Calibration curves were obtained corresponding to analyte concentrations ranging from 40-600 μg mL-1 for the colorimetric assay, and from 1-200 μg mL-1 for chemiluminescence assay.

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author
; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Analyst
volume
125
issue
1
pages
4 pages
publisher
Royal Society of Chemistry
external identifiers
  • scopus:0033971170
ISSN
0003-2654
DOI
10.1039/a908871g
language
English
LU publication?
yes
id
8a781b3e-e5b2-4e75-b4fd-dc749b56ff46
date added to LUP
2019-06-18 09:03:29
date last changed
2020-10-20 01:15:38
@article{8a781b3e-e5b2-4e75-b4fd-dc749b56ff46,
  abstract     = {<p>Based on a molecularly imprinted polymer, a competitive binding assay analogous to competitive enzyme immunoassay has been developed. The assay is specific for the herbicide 2,4-dichlorophenoxyacetic acid and uses, for the first time, an enzyme-labelled conjugate as a tracer. The label tobacco peroxidase allowed for colorimetric and chemiluminescence detection. The molecularly imprinted polymer was synthesised in the form of microspheres by precipitation polymerisation. The polymer efficiently and selectively bound the analyte in aqueous solution. Calibration curves were obtained corresponding to analyte concentrations ranging from 40-600 μg mL<sup>-1</sup> for the colorimetric assay, and from 1-200 μg mL<sup>-1</sup> for chemiluminescence assay.</p>},
  author       = {Surugiu, Ioana and Ye, Lei and Yilmaz, Ecevit and Dzgoev, Anatoli and Danielsson, Bengt and Mosbach, Klaus and Haupt, Karsten},
  issn         = {0003-2654},
  language     = {eng},
  month        = {01},
  number       = {1},
  pages        = {13--16},
  publisher    = {Royal Society of Chemistry},
  series       = {Analyst},
  title        = {An enzyme-linked molecularly imprinted sorbent assay},
  url          = {http://dx.doi.org/10.1039/a908871g},
  doi          = {10.1039/a908871g},
  volume       = {125},
  year         = {2000},
}