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Direct comparison between in vivo and in vitro microsized particle phagocytosis assays in Drosophila melanogaster

Adolfsson, K. LU ; Abariute, L. LU ; Dabkowska, A. P. LU ; Schneider, M. LU ; Häcker, U. LU and Prinz, C. N. LU (2018) In Toxicology in Vitro 46. p.213-218
Abstract

The effects of micro and nanoparticles on the innate immune system have been widely investigated and a general lack of agreement between in vivo and in vitro assays has been observed. In order to determine the origin of these discrepancies, there is a need for comparing the results of in vivo and in vitro phagocytosis assays obtained using the same particles and same immune cells. Here, we establish an in vivo polystyrene microsized particle phagocytosis assay in Drosophila melanogaster and compare it with an in vitro assay consisting of exposing the same immune cells in culture to the same particles. The distribution of number of phagocytized beads per cell was shifted to lower numbers of beads per cell in the case of the in vitro... (More)

The effects of micro and nanoparticles on the innate immune system have been widely investigated and a general lack of agreement between in vivo and in vitro assays has been observed. In order to determine the origin of these discrepancies, there is a need for comparing the results of in vivo and in vitro phagocytosis assays obtained using the same particles and same immune cells. Here, we establish an in vivo polystyrene microsized particle phagocytosis assay in Drosophila melanogaster and compare it with an in vitro assay consisting of exposing the same immune cells in culture to the same particles. The distribution of number of phagocytized beads per cell was shifted to lower numbers of beads per cell in the case of the in vitro assay compared to the in vivo assay, which we suggest is partly due to a reduced amount of membrane available in cultured cells.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Drosophila melanogaster, Immune cells, Particle toxicology, Phagocytosis, Phagocytosis assay, Polystyrene beads
in
Toxicology in Vitro
volume
46
pages
6 pages
publisher
Elsevier
external identifiers
  • scopus:85031785616
ISSN
0887-2333
DOI
10.1016/j.tiv.2017.10.014
language
English
LU publication?
yes
id
8ac8300b-06f1-4888-8593-12cbc8d75734
date added to LUP
2017-10-30 10:26:58
date last changed
2018-01-07 12:24:13
@article{8ac8300b-06f1-4888-8593-12cbc8d75734,
  abstract     = {<p>The effects of micro and nanoparticles on the innate immune system have been widely investigated and a general lack of agreement between in vivo and in vitro assays has been observed. In order to determine the origin of these discrepancies, there is a need for comparing the results of in vivo and in vitro phagocytosis assays obtained using the same particles and same immune cells. Here, we establish an in vivo polystyrene microsized particle phagocytosis assay in Drosophila melanogaster and compare it with an in vitro assay consisting of exposing the same immune cells in culture to the same particles. The distribution of number of phagocytized beads per cell was shifted to lower numbers of beads per cell in the case of the in vitro assay compared to the in vivo assay, which we suggest is partly due to a reduced amount of membrane available in cultured cells.</p>},
  author       = {Adolfsson, K. and Abariute, L. and Dabkowska, A. P. and Schneider, M. and Häcker, U. and Prinz, C. N.},
  issn         = {0887-2333},
  keyword      = {Drosophila melanogaster,Immune cells,Particle toxicology,Phagocytosis,Phagocytosis assay,Polystyrene beads},
  language     = {eng},
  month        = {02},
  pages        = {213--218},
  publisher    = {Elsevier},
  series       = {Toxicology in Vitro},
  title        = {Direct comparison between in vivo and in vitro microsized particle phagocytosis assays in Drosophila melanogaster},
  url          = {http://dx.doi.org/10.1016/j.tiv.2017.10.014},
  volume       = {46},
  year         = {2018},
}