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MiR-378a suppresses tenogenic differentiation and tendon repair by targeting at TGF-β2

Liu, Yang LU ; Feng, Lu; Xu, Jia; Yang, Zhengmeng; Wu, Tianyi; Zhang, Jiajun; Shi, Liu; Zhu, Dahai; Zhang, Jinfang and Li, Gang (2019) In Stem Cell Research and Therapy 10(1).
Abstract

Background: Tendons are a crucial component of the musculoskeletal system and responsible for transmission forces derived from muscle to bone. Patients with tendon injuries are often observed with decreased collagen production and matrix degeneration, and healing of tendon injuries remains a challenge as a result of limited understanding of tendon biology. Recent studies highlight the contribution of miR-378a on the regulation gene expression during tendon differentiation. Methods: We examined the tendon microstructure and tendon repair with using miR-378a knock-in transgenic mice, and the tendon-derived stem cells were also isolated from transgenic mice to study their tenogenic differentiation ability. Meanwhile, the expression levels... (More)

Background: Tendons are a crucial component of the musculoskeletal system and responsible for transmission forces derived from muscle to bone. Patients with tendon injuries are often observed with decreased collagen production and matrix degeneration, and healing of tendon injuries remains a challenge as a result of limited understanding of tendon biology. Recent studies highlight the contribution of miR-378a on the regulation gene expression during tendon differentiation. Methods: We examined the tendon microstructure and tendon repair with using miR-378a knock-in transgenic mice, and the tendon-derived stem cells were also isolated from transgenic mice to study their tenogenic differentiation ability. Meanwhile, the expression levels of tenogenic markers were also examined in mouse tendon-derived stem cells transfected with miR-378a mimics during tenogenic differentiation. With using online prediction software and luciferase reporter assay, the binding target of miR-378a was also studied. Results: Our results indicated miR-378a impairs tenogenic differentiation and tendon repair by inhibition collagen and extracellular matrix production both in vitro and in vivo. We also demonstrated that miR-378a exert its inhibitory role during tenogenic differentiation through binding at TGFβ2 by luciferase reporter assay and western blot. Conclusions: Our investigation suggests that miR-378a could be considered as a new potential biomarker for tendon injury diagnosis or drug target for a possible therapeutic approach in future clinical practice.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Stem Cell Research and Therapy
volume
10
issue
1
publisher
BioMed Central
external identifiers
  • scopus:85063777619
ISSN
1757-6512
DOI
10.1186/s13287-019-1216-y
language
English
LU publication?
yes
id
8b0527e3-52c7-431e-8af1-4bccd09ae312
date added to LUP
2019-04-23 14:23:04
date last changed
2019-05-14 04:53:56
@article{8b0527e3-52c7-431e-8af1-4bccd09ae312,
  abstract     = {<p>Background: Tendons are a crucial component of the musculoskeletal system and responsible for transmission forces derived from muscle to bone. Patients with tendon injuries are often observed with decreased collagen production and matrix degeneration, and healing of tendon injuries remains a challenge as a result of limited understanding of tendon biology. Recent studies highlight the contribution of miR-378a on the regulation gene expression during tendon differentiation. Methods: We examined the tendon microstructure and tendon repair with using miR-378a knock-in transgenic mice, and the tendon-derived stem cells were also isolated from transgenic mice to study their tenogenic differentiation ability. Meanwhile, the expression levels of tenogenic markers were also examined in mouse tendon-derived stem cells transfected with miR-378a mimics during tenogenic differentiation. With using online prediction software and luciferase reporter assay, the binding target of miR-378a was also studied. Results: Our results indicated miR-378a impairs tenogenic differentiation and tendon repair by inhibition collagen and extracellular matrix production both in vitro and in vivo. We also demonstrated that miR-378a exert its inhibitory role during tenogenic differentiation through binding at TGFβ2 by luciferase reporter assay and western blot. Conclusions: Our investigation suggests that miR-378a could be considered as a new potential biomarker for tendon injury diagnosis or drug target for a possible therapeutic approach in future clinical practice.</p>},
  articleno    = {108},
  author       = {Liu, Yang and Feng, Lu and Xu, Jia and Yang, Zhengmeng and Wu, Tianyi and Zhang, Jiajun and Shi, Liu and Zhu, Dahai and Zhang, Jinfang and Li, Gang},
  issn         = {1757-6512},
  language     = {eng},
  number       = {1},
  publisher    = {BioMed Central},
  series       = {Stem Cell Research and Therapy},
  title        = {MiR-378a suppresses tenogenic differentiation and tendon repair by targeting at TGF-β2},
  url          = {http://dx.doi.org/10.1186/s13287-019-1216-y},
  volume       = {10},
  year         = {2019},
}