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Hydrogen and polyhydroxybutyrate production from wheat straw hydrolysate using Caldicellulosiruptor species and Ralstonia eutropha in a coupled process

Soto, Luis Romero LU ; Byrne, Eoin LU ; van Niel, Ed W.J. LU ; Sayed, Mahmoud LU ; Villanueva, Cristhian Carrasco and Hatti-Kaul, Rajni LU (2019) In Bioresource Technology 272. p.259-266
Abstract

This report presents an integrated biorefinery concept in which wheat straw hydrolysate was treated with co-cultures of osmotolerant thermophilic bacterial strains, Caldicellulosiruptor saccharolyticus and C. owensensis to obtain hydrogen, while the liquid effluent containing acetate and residual glucose was used as feed for polyhydroxybutyrate (PHB) production by Ralstonia eutropha. The Caldicellulosiruptor spp. co-culture consumed 10.8 g/L of pretreated straw sugars, glucose and xylose, producing 134 mmol H2/L. PHB accumulation by R. eutropha was first studied in minimal salts medium using acetate with/without glucose as carbon source. Addition of salts promoted cell growth and PHB production in the effluent. Fed-batch... (More)

This report presents an integrated biorefinery concept in which wheat straw hydrolysate was treated with co-cultures of osmotolerant thermophilic bacterial strains, Caldicellulosiruptor saccharolyticus and C. owensensis to obtain hydrogen, while the liquid effluent containing acetate and residual glucose was used as feed for polyhydroxybutyrate (PHB) production by Ralstonia eutropha. The Caldicellulosiruptor spp. co-culture consumed 10.8 g/L of pretreated straw sugars, glucose and xylose, producing 134 mmol H2/L. PHB accumulation by R. eutropha was first studied in minimal salts medium using acetate with/without glucose as carbon source. Addition of salts promoted cell growth and PHB production in the effluent. Fed-batch cultivation in a nitrogen limited medium with 40% (v/v) aeration resulted in a cell density of 15.1 g/L with PHB content of 80.1% w/w and PHB concentration of 12.1 g/L, while 20% aeration gave a cell density of 11.3 g/L with 83.4% w/w PHB content and 9.4 g/L PHB concentration.

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author
; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Caldicellulosiruptor species, Hydrogen, Integrated biorefinery, Polyhydroxybutyrate, Ralstonia eutropha, Wheat straw hydrolysate
in
Bioresource Technology
volume
272
pages
8 pages
publisher
Elsevier
external identifiers
  • scopus:85055089724
  • pmid:30352368
ISSN
0960-8524
DOI
10.1016/j.biortech.2018.09.142
project
Farm2Furan: Surplus agricultural feedstocks to furanics
language
English
LU publication?
yes
id
8b1e40ea-d994-4d70-96c7-622790976d6b
date added to LUP
2018-11-14 08:37:57
date last changed
2023-04-08 20:52:58
@article{8b1e40ea-d994-4d70-96c7-622790976d6b,
  abstract     = {{<p>This report presents an integrated biorefinery concept in which wheat straw hydrolysate was treated with co-cultures of osmotolerant thermophilic bacterial strains, Caldicellulosiruptor saccharolyticus and C. owensensis to obtain hydrogen, while the liquid effluent containing acetate and residual glucose was used as feed for polyhydroxybutyrate (PHB) production by Ralstonia eutropha. The Caldicellulosiruptor spp. co-culture consumed 10.8 g/L of pretreated straw sugars, glucose and xylose, producing 134 mmol H<sub>2</sub>/L. PHB accumulation by R. eutropha was first studied in minimal salts medium using acetate with/without glucose as carbon source. Addition of salts promoted cell growth and PHB production in the effluent. Fed-batch cultivation in a nitrogen limited medium with 40% (v/v) aeration resulted in a cell density of 15.1 g/L with PHB content of 80.1% w/w and PHB concentration of 12.1 g/L, while 20% aeration gave a cell density of 11.3 g/L with 83.4% w/w PHB content and 9.4 g/L PHB concentration.</p>}},
  author       = {{Soto, Luis Romero and Byrne, Eoin and van Niel, Ed W.J. and Sayed, Mahmoud and Villanueva, Cristhian Carrasco and Hatti-Kaul, Rajni}},
  issn         = {{0960-8524}},
  keywords     = {{Caldicellulosiruptor species; Hydrogen; Integrated biorefinery; Polyhydroxybutyrate; Ralstonia eutropha; Wheat straw hydrolysate}},
  language     = {{eng}},
  pages        = {{259--266}},
  publisher    = {{Elsevier}},
  series       = {{Bioresource Technology}},
  title        = {{Hydrogen and polyhydroxybutyrate production from wheat straw hydrolysate using Caldicellulosiruptor species and Ralstonia eutropha in a coupled process}},
  url          = {{http://dx.doi.org/10.1016/j.biortech.2018.09.142}},
  doi          = {{10.1016/j.biortech.2018.09.142}},
  volume       = {{272}},
  year         = {{2019}},
}