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β-amyloid peptides inhibit acetylcholine release from cholinergic presynaptic nerve endings isolated from an electric ray

Satoh, Yasushi; Hirakura, Yutaka; Shibayama, Sotaro LU ; Hirashima, Naohide; Suzuki, Toshiharu and Kirino, Yutaka (2001) In Neuroscience Letters 302(2-3). p.97-100
Abstract

We investigated the effects of β-amyloid (Aβ) peptides on cholinergic synaptosomes isolated from the electric organ of the Japanese marine ray Narke japonica. Fresh and pre-incubated solutions of Aβ(1-42) inhibited acetylcholine (ACh) release from the synaptosomes evoked by high [K+] depolarization when incubated with synaptosomes for 10 min before the depolarizing stimulus. A freshly prepared solution of Aβ(1-40) did not inhibit the evoked ACh release, but prolonged pre-incubation of Aβ(1-40) solution caused the inhibition. Aβ(1-15) neither in fresh nor pre-incubated solution inhibited. These results have demonstrated that Aβ peptides can acutely inhibit the depolarization-evoked release of ACh by acting directly on... (More)

We investigated the effects of β-amyloid (Aβ) peptides on cholinergic synaptosomes isolated from the electric organ of the Japanese marine ray Narke japonica. Fresh and pre-incubated solutions of Aβ(1-42) inhibited acetylcholine (ACh) release from the synaptosomes evoked by high [K+] depolarization when incubated with synaptosomes for 10 min before the depolarizing stimulus. A freshly prepared solution of Aβ(1-40) did not inhibit the evoked ACh release, but prolonged pre-incubation of Aβ(1-40) solution caused the inhibition. Aβ(1-15) neither in fresh nor pre-incubated solution inhibited. These results have demonstrated that Aβ peptides can acutely inhibit the depolarization-evoked release of ACh by acting directly on cholinergic presynaptic nerve endings. The electrophoresis analysis showed a strong correlation between Aβ aggregation and its inhibition for ACh release.

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author
publishing date
type
Contribution to journal
publication status
published
subject
keywords
β-Amyloid, aggregation, cholinergic neurons, electric organ, high [K] depolarization, nerve terminals, synaptosomes
in
Neuroscience Letters
volume
302
issue
2-3
pages
4 pages
publisher
Elsevier
external identifiers
  • scopus:0035917827
ISSN
0304-3940
DOI
10.1016/S0304-3940(01)01665-2
language
English
LU publication?
no
id
8b2b02ad-4b10-44b2-84e3-8f9678f49c7b
date added to LUP
2017-04-19 16:41:02
date last changed
2018-01-07 11:59:49
@article{8b2b02ad-4b10-44b2-84e3-8f9678f49c7b,
  abstract     = {<p>We investigated the effects of β-amyloid (Aβ) peptides on cholinergic synaptosomes isolated from the electric organ of the Japanese marine ray Narke japonica. Fresh and pre-incubated solutions of Aβ(1-42) inhibited acetylcholine (ACh) release from the synaptosomes evoked by high [K<sup>+</sup>] depolarization when incubated with synaptosomes for 10 min before the depolarizing stimulus. A freshly prepared solution of Aβ(1-40) did not inhibit the evoked ACh release, but prolonged pre-incubation of Aβ(1-40) solution caused the inhibition. Aβ(1-15) neither in fresh nor pre-incubated solution inhibited. These results have demonstrated that Aβ peptides can acutely inhibit the depolarization-evoked release of ACh by acting directly on cholinergic presynaptic nerve endings. The electrophoresis analysis showed a strong correlation between Aβ aggregation and its inhibition for ACh release.</p>},
  author       = {Satoh, Yasushi and Hirakura, Yutaka and Shibayama, Sotaro and Hirashima, Naohide and Suzuki, Toshiharu and Kirino, Yutaka},
  issn         = {0304-3940},
  keyword      = {β-Amyloid,aggregation,cholinergic neurons,electric organ,high [K] depolarization,nerve terminals,synaptosomes},
  language     = {eng},
  month        = {04},
  number       = {2-3},
  pages        = {97--100},
  publisher    = {Elsevier},
  series       = {Neuroscience Letters},
  title        = {β-amyloid peptides inhibit acetylcholine release from cholinergic presynaptic nerve endings isolated from an electric ray},
  url          = {http://dx.doi.org/10.1016/S0304-3940(01)01665-2},
  volume       = {302},
  year         = {2001},
}