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Characteristics of GABAA channels in rat dentate gyrus

Birnir, Bryndis LU ; Everitt, A B and Gage, P W (1994) In Journal of Membrane Biology 142(1). p.93-102
Abstract
Single channel currents were activated by GABA (0.5 to 5 microM) in cell-attached and inside-out patches from cells in the dentate gyrus of rat hippocampal slices. The currents reversed at the chloride equilibrium potential and were blocked by bicuculline (100 microM). Several different kinds of channel were seen: high conductance and low conductance, rectifying and "nonrectifying." Channels had multiple conductance states. The open probability (Po) of channels was greater at depolarized than at hyperpolarized potentials and the relationship between Po and potential could be fitted with a Boltzmann equation with equivalent valency (z) of 1. The combination of outward rectification and potential-dependent open probability gave very little... (More)
Single channel currents were activated by GABA (0.5 to 5 microM) in cell-attached and inside-out patches from cells in the dentate gyrus of rat hippocampal slices. The currents reversed at the chloride equilibrium potential and were blocked by bicuculline (100 microM). Several different kinds of channel were seen: high conductance and low conductance, rectifying and "nonrectifying." Channels had multiple conductance states. The open probability (Po) of channels was greater at depolarized than at hyperpolarized potentials and the relationship between Po and potential could be fitted with a Boltzmann equation with equivalent valency (z) of 1. The combination of outward rectification and potential-dependent open probability gave very little chloride current at hyperpolarized potentials but steeply increasing current with depolarization, useful properties for a tonic inhibitory mechanism. (Less)
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publishing date
type
Contribution to journal
publication status
published
subject
keywords
GABAA channels, Dentate gyrus, Hippocampal slice, Outward rectification
in
Journal of Membrane Biology
volume
142
issue
1
pages
93 - 102
publisher
Springer
external identifiers
  • pmid:7707357
  • scopus:0028173169
ISSN
0022-2631
DOI
10.1007/BF00233386
language
English
LU publication?
no
additional info
The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: GABA Channels in Physiology and Pharmacology (013241570)
id
8b404322-c706-4954-ac69-694b4a8ab97c (old id 1107995)
date added to LUP
2016-04-01 16:31:25
date last changed
2020-06-21 04:46:26
@article{8b404322-c706-4954-ac69-694b4a8ab97c,
  abstract     = {Single channel currents were activated by GABA (0.5 to 5 microM) in cell-attached and inside-out patches from cells in the dentate gyrus of rat hippocampal slices. The currents reversed at the chloride equilibrium potential and were blocked by bicuculline (100 microM). Several different kinds of channel were seen: high conductance and low conductance, rectifying and "nonrectifying." Channels had multiple conductance states. The open probability (Po) of channels was greater at depolarized than at hyperpolarized potentials and the relationship between Po and potential could be fitted with a Boltzmann equation with equivalent valency (z) of 1. The combination of outward rectification and potential-dependent open probability gave very little chloride current at hyperpolarized potentials but steeply increasing current with depolarization, useful properties for a tonic inhibitory mechanism.},
  author       = {Birnir, Bryndis and Everitt, A B and Gage, P W},
  issn         = {0022-2631},
  language     = {eng},
  number       = {1},
  pages        = {93--102},
  publisher    = {Springer},
  series       = {Journal of Membrane Biology},
  title        = {Characteristics of GABAA channels in rat dentate gyrus},
  url          = {http://dx.doi.org/10.1007/BF00233386},
  doi          = {10.1007/BF00233386},
  volume       = {142},
  year         = {1994},
}