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A simple adaptation to a protein crystallography station to facilitate difference X-ray scattering studies

Sharma, Amit; Berntsen, Peter; Harimoorthy, Rajiv; Appio, Roberto LU ; Sjöhamn, Jennie; Järvå, Michael; Björling, Alexander LU ; Hammarin, Greger; Westenhoff, Sebastian and Brändén, Gisela, et al. (2019) In Journal of Applied Crystallography 52(2). p.378-386
Abstract

The X-ray crystallography station I911-2 at MAXLab II (Lund, Sweden) has been adapted to enable difference small- and wide-angle X-ray scattering (SAXS/WAXS) data to be recorded. Modifications to the beamline included a customized flow cell, a motorized flow cell holder, a helium cone, a beam stop, a sample stage and a sample delivery system. This setup incorporated external devices such as infrared lasers, LEDs and reaction mixers to induce conformational changes in macromolecules. This platform was evaluated through proof-of-principle experiments capturing light-induced conformational changes in phytochromes. A difference WAXS signature of conformational changes in a plant aquaporin was also demonstrated using caged calcium.

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organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
difference X-ray scattering, protein structural changes
in
Journal of Applied Crystallography
volume
52
issue
2
pages
378 - 386
publisher
International Union of Crystallography
external identifiers
  • scopus:85063649408
ISSN
1600-5767
DOI
10.1107/S1600576719001900
language
English
LU publication?
yes
id
8b64864c-d5a4-4f75-a653-eaf5b2a25ac3
date added to LUP
2019-04-11 13:15:38
date last changed
2019-06-28 10:49:13
@article{8b64864c-d5a4-4f75-a653-eaf5b2a25ac3,
  abstract     = {<p>The X-ray crystallography station I911-2 at MAXLab II (Lund, Sweden) has been adapted to enable difference small- and wide-angle X-ray scattering (SAXS/WAXS) data to be recorded. Modifications to the beamline included a customized flow cell, a motorized flow cell holder, a helium cone, a beam stop, a sample stage and a sample delivery system. This setup incorporated external devices such as infrared lasers, LEDs and reaction mixers to induce conformational changes in macromolecules. This platform was evaluated through proof-of-principle experiments capturing light-induced conformational changes in phytochromes. A difference WAXS signature of conformational changes in a plant aquaporin was also demonstrated using caged calcium.</p>},
  author       = {Sharma, Amit and Berntsen, Peter and Harimoorthy, Rajiv and Appio, Roberto and Sjöhamn, Jennie and Järvå, Michael and Björling, Alexander and Hammarin, Greger and Westenhoff, Sebastian and Brändén, Gisela and Neutze, Richard},
  issn         = {1600-5767},
  keyword      = {difference X-ray scattering,protein structural changes},
  language     = {eng},
  number       = {2},
  pages        = {378--386},
  publisher    = {International Union of Crystallography},
  series       = {Journal of Applied Crystallography},
  title        = {A simple adaptation to a protein crystallography station to facilitate difference X-ray scattering studies},
  url          = {http://dx.doi.org/10.1107/S1600576719001900},
  volume       = {52},
  year         = {2019},
}