A simple adaptation to a protein crystallography station to facilitate difference X-ray scattering studies
(2019) In Journal of Applied Crystallography 52(2). p.378-386- Abstract
The X-ray crystallography station I911-2 at MAXLab II (Lund, Sweden) has been adapted to enable difference small- and wide-angle X-ray scattering (SAXS/WAXS) data to be recorded. Modifications to the beamline included a customized flow cell, a motorized flow cell holder, a helium cone, a beam stop, a sample stage and a sample delivery system. This setup incorporated external devices such as infrared lasers, LEDs and reaction mixers to induce conformational changes in macromolecules. This platform was evaluated through proof-of-principle experiments capturing light-induced conformational changes in phytochromes. A difference WAXS signature of conformational changes in a plant aquaporin was also demonstrated using caged calcium.
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- author
- organization
- publishing date
- 2019
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- difference X-ray scattering, protein structural changes
- in
- Journal of Applied Crystallography
- volume
- 52
- issue
- 2
- pages
- 378 - 386
- publisher
- International Union of Crystallography
- external identifiers
-
- pmid:30996717
- scopus:85063649408
- ISSN
- 1600-5767
- DOI
- 10.1107/S1600576719001900
- language
- English
- LU publication?
- yes
- id
- 8b64864c-d5a4-4f75-a653-eaf5b2a25ac3
- date added to LUP
- 2019-04-11 13:15:38
- date last changed
- 2024-03-19 05:13:27
@article{8b64864c-d5a4-4f75-a653-eaf5b2a25ac3, abstract = {{<p>The X-ray crystallography station I911-2 at MAXLab II (Lund, Sweden) has been adapted to enable difference small- and wide-angle X-ray scattering (SAXS/WAXS) data to be recorded. Modifications to the beamline included a customized flow cell, a motorized flow cell holder, a helium cone, a beam stop, a sample stage and a sample delivery system. This setup incorporated external devices such as infrared lasers, LEDs and reaction mixers to induce conformational changes in macromolecules. This platform was evaluated through proof-of-principle experiments capturing light-induced conformational changes in phytochromes. A difference WAXS signature of conformational changes in a plant aquaporin was also demonstrated using caged calcium.</p>}}, author = {{Sharma, Amit and Berntsen, Peter and Harimoorthy, Rajiv and Appio, Roberto and Sjöhamn, Jennie and Järvå, Michael and Björling, Alexander and Hammarin, Greger and Westenhoff, Sebastian and Brändén, Gisela and Neutze, Richard}}, issn = {{1600-5767}}, keywords = {{difference X-ray scattering; protein structural changes}}, language = {{eng}}, number = {{2}}, pages = {{378--386}}, publisher = {{International Union of Crystallography}}, series = {{Journal of Applied Crystallography}}, title = {{A simple adaptation to a protein crystallography station to facilitate difference X-ray scattering studies}}, url = {{http://dx.doi.org/10.1107/S1600576719001900}}, doi = {{10.1107/S1600576719001900}}, volume = {{52}}, year = {{2019}}, }