Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

Differential regulation of alpha and beta chains of C4b-binding protein during acute-phase response resulting in stable plasma levels of free anticoagulant protein S

Garcia de Frutos, Pablo LU ; Alim, R ; Härdig, Ylva ; Zöller, Bengt LU orcid and Dahlbäck, Björn LU (1994) In Blood 84(3). p.815-822
Abstract

Regulation of C4b-binding protein (C4BP) isoforms during acute phase and its relationship to the plasma concentration of free protein S was elucidated. An assay for beta chain containing C4BP (C4BP beta+) was developed and the concentrations of total C4BP, C4BP beta+, total, free, and bound protein S were measured in patients with acute-phase response. Even though total C4BP was increased to 162% (mean value) of controls, the corresponding value of C4BP beta+ was only 122%. In the acute-phase group, total protein S was increased to the same extent as C4BP beta+ (mean value of 124%), whereas free protein S was not decreased. In controls, total and bound protein S correlated with total C4BP and C4BP beta+. However, in the acute-phase... (More)

Regulation of C4b-binding protein (C4BP) isoforms during acute phase and its relationship to the plasma concentration of free protein S was elucidated. An assay for beta chain containing C4BP (C4BP beta+) was developed and the concentrations of total C4BP, C4BP beta+, total, free, and bound protein S were measured in patients with acute-phase response. Even though total C4BP was increased to 162% (mean value) of controls, the corresponding value of C4BP beta+ was only 122%. In the acute-phase group, total protein S was increased to the same extent as C4BP beta+ (mean value of 124%), whereas free protein S was not decreased. In controls, total and bound protein S correlated with total C4BP and C4BP beta+. However, in the acute-phase group, the correlation between bound protein S and total C4BP was lost, although the correlation between C4BP beta+ and protein S remained. The present results suggest stable levels of free protein S during acute phase to be the result of differential regulation of C4BP alpha- and beta-chain expression, and the concentration of free protein S to be the resulting molar excess of protein S over C4BP beta+. This mechanism ensures functional levels of free anticoagulant protein S despite high levels of C4BP.

(Less)
Please use this url to cite or link to this publication:
author
; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Acute-Phase Proteins, Acute-Phase Reaction, Antibodies, Monoclonal, Calcium, Carrier Proteins, Complement C4b, Complement Inactivator Proteins, Glycoproteins, Humans, Macromolecular Substances, Protein S, Journal Article, Research Support, Non-U.S. Gov't
in
Blood
volume
84
issue
3
pages
8 pages
publisher
American Society of Hematology
external identifiers
  • pmid:7519078
  • scopus:0028103281
ISSN
1528-0020
language
English
LU publication?
yes
id
8bad4052-e453-4a54-870c-4dccca3199f4 (old id 1894793)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/7519078
http://bloodjournal.hematologylibrary.org/content/84/3/815.long
date added to LUP
2016-04-04 13:36:06
date last changed
2021-03-21 04:40:47
@article{8bad4052-e453-4a54-870c-4dccca3199f4,
  abstract     = {{<p>Regulation of C4b-binding protein (C4BP) isoforms during acute phase and its relationship to the plasma concentration of free protein S was elucidated. An assay for beta chain containing C4BP (C4BP beta+) was developed and the concentrations of total C4BP, C4BP beta+, total, free, and bound protein S were measured in patients with acute-phase response. Even though total C4BP was increased to 162% (mean value) of controls, the corresponding value of C4BP beta+ was only 122%. In the acute-phase group, total protein S was increased to the same extent as C4BP beta+ (mean value of 124%), whereas free protein S was not decreased. In controls, total and bound protein S correlated with total C4BP and C4BP beta+. However, in the acute-phase group, the correlation between bound protein S and total C4BP was lost, although the correlation between C4BP beta+ and protein S remained. The present results suggest stable levels of free protein S during acute phase to be the result of differential regulation of C4BP alpha- and beta-chain expression, and the concentration of free protein S to be the resulting molar excess of protein S over C4BP beta+. This mechanism ensures functional levels of free anticoagulant protein S despite high levels of C4BP.</p>}},
  author       = {{Garcia de Frutos, Pablo and Alim, R and Härdig, Ylva and Zöller, Bengt and Dahlbäck, Björn}},
  issn         = {{1528-0020}},
  keywords     = {{Acute-Phase Proteins; Acute-Phase Reaction; Antibodies, Monoclonal; Calcium; Carrier Proteins; Complement C4b; Complement Inactivator Proteins; Glycoproteins; Humans; Macromolecular Substances; Protein S; Journal Article; Research Support, Non-U.S. Gov't}},
  language     = {{eng}},
  number       = {{3}},
  pages        = {{815--822}},
  publisher    = {{American Society of Hematology}},
  series       = {{Blood}},
  title        = {{Differential regulation of alpha and beta chains of C4b-binding protein during acute-phase response resulting in stable plasma levels of free anticoagulant protein S}},
  url          = {{http://www.ncbi.nlm.nih.gov/pubmed/7519078}},
  volume       = {{84}},
  year         = {{1994}},
}