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Gene profiling reveals a contact allergy signature in most positive Amerchol L-101 patch-test reactions

Ljungberg Silic, Linda LU ; Lefevre, Marine Alexia ; Bergendorff, Ola LU ; De Bernard, Simon ; Nourikyan, Julien ; Buffat, Laurent ; Nosbaum, Audrey ; Bruze, Magnus LU ; Nicolas, Jean François and Svedman, Cecilia LU , et al. (2022) In Contact Dermatitis 87(1). p.40-52
Abstract

Background: Diagnosis of contact allergy (CA) to Amerchol L-101 (AL-101), a marker for lanolin allergy, is problematic. Positive patch-test reactions are frequently doubtful or weakly positive and difficult to associate with clinical relevance. Objective: To gain further insight on the allergic or irritant nature of skin reactions induced by AL-101 patch test. Methods: We re-tested in a dose–response fashion, 10 subjects with AL-101 CA and performed comprehensive transcriptomic analysis (gene arrays, quantitative real-time polymerase chain reaction [qRT-PCR]) of samples of their skin reactions. Results: Eight of the 10 CA subjects reacted positively upon re-test, whereas two did not react. Most of AL-101 positive patch tests expressed... (More)

Background: Diagnosis of contact allergy (CA) to Amerchol L-101 (AL-101), a marker for lanolin allergy, is problematic. Positive patch-test reactions are frequently doubtful or weakly positive and difficult to associate with clinical relevance. Objective: To gain further insight on the allergic or irritant nature of skin reactions induced by AL-101 patch test. Methods: We re-tested in a dose–response fashion, 10 subjects with AL-101 CA and performed comprehensive transcriptomic analysis (gene arrays, quantitative real-time polymerase chain reaction [qRT-PCR]) of samples of their skin reactions. Results: Eight of the 10 CA subjects reacted positively upon re-test, whereas two did not react. Most of AL-101 positive patch tests expressed an allergy signature with strong activation of gene modules associated with adaptive immunity and downregulation of cornification pathway genes. In addition, the breadth of gene modulation correlated with the magnitude of patch-test reactions and the concentration of AL-101 applied. However, we observed that some of the positive patch-test reactions to AL-101 expressed no/few allergy biomarkers, suggesting the induction of an irritant skin inflammation in these samples. Conclusions: This study confirms that AL-101 is an allergen that can cause both contact allergy and contact irritation. Our results also highlight that molecular profiling might help to strengthen clinical diagnosis.

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organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
allergic contact dermatitis, Amerchol L-101, contact allergy, irritant contact dermatitis, irritant reaction, lanolin, patch testing, transcriptomic profiling
in
Contact Dermatitis
volume
87
issue
1
pages
40 - 52
publisher
Wiley-Blackwell
external identifiers
  • pmid:35184302
  • scopus:85127294655
ISSN
0105-1873
DOI
10.1111/cod.14077
language
English
LU publication?
yes
id
8bad763b-d1b0-40d0-9e1e-3a8d5b17eba1
date added to LUP
2022-06-07 11:38:08
date last changed
2024-04-18 04:29:20
@article{8bad763b-d1b0-40d0-9e1e-3a8d5b17eba1,
  abstract     = {{<p>Background: Diagnosis of contact allergy (CA) to Amerchol L-101 (AL-101), a marker for lanolin allergy, is problematic. Positive patch-test reactions are frequently doubtful or weakly positive and difficult to associate with clinical relevance. Objective: To gain further insight on the allergic or irritant nature of skin reactions induced by AL-101 patch test. Methods: We re-tested in a dose–response fashion, 10 subjects with AL-101 CA and performed comprehensive transcriptomic analysis (gene arrays, quantitative real-time polymerase chain reaction [qRT-PCR]) of samples of their skin reactions. Results: Eight of the 10 CA subjects reacted positively upon re-test, whereas two did not react. Most of AL-101 positive patch tests expressed an allergy signature with strong activation of gene modules associated with adaptive immunity and downregulation of cornification pathway genes. In addition, the breadth of gene modulation correlated with the magnitude of patch-test reactions and the concentration of AL-101 applied. However, we observed that some of the positive patch-test reactions to AL-101 expressed no/few allergy biomarkers, suggesting the induction of an irritant skin inflammation in these samples. Conclusions: This study confirms that AL-101 is an allergen that can cause both contact allergy and contact irritation. Our results also highlight that molecular profiling might help to strengthen clinical diagnosis.</p>}},
  author       = {{Ljungberg Silic, Linda and Lefevre, Marine Alexia and Bergendorff, Ola and De Bernard, Simon and Nourikyan, Julien and Buffat, Laurent and Nosbaum, Audrey and Bruze, Magnus and Nicolas, Jean François and Svedman, Cecilia and Vocanson, Marc}},
  issn         = {{0105-1873}},
  keywords     = {{allergic contact dermatitis; Amerchol L-101; contact allergy; irritant contact dermatitis; irritant reaction; lanolin; patch testing; transcriptomic profiling}},
  language     = {{eng}},
  number       = {{1}},
  pages        = {{40--52}},
  publisher    = {{Wiley-Blackwell}},
  series       = {{Contact Dermatitis}},
  title        = {{Gene profiling reveals a contact allergy signature in most positive Amerchol L-101 patch-test reactions}},
  url          = {{http://dx.doi.org/10.1111/cod.14077}},
  doi          = {{10.1111/cod.14077}},
  volume       = {{87}},
  year         = {{2022}},
}