Interaction of sugars, polysaccharides and cells with boronate-containing copolymers: from solution to polymer brushes
(2006) Bioaffinity 2005 19(4). p.322-331- Abstract
- Interaction of mono- and disaccharides, polysaccharide particles and yeast cells with boronate-containing copolymers (BCC) of N-acryloyl-m-aminophenylboronic acid (NAAPBA) with NN-dimethylacrylamide (DMAA) or N-isopropylacrylamide (NIPAM) was studied. The binding of saccharides to BCC of NIPAM resulted in a shift of its phase transition temperature (Delta T-p), which provided a quantitative measure for the complex formation. Among the sugars typical of non-reducing ends of glycoproteins the Delta T-p decreased in the order: N-acetylneuraminic acid > xylose approximate to galactose > mannose approximate to fucose >> N-acetylglucosamine. Strong specific adsorption of the BCC on the cross-linked agarose gel Sepharose CL-6B (15-30... (More)
- Interaction of mono- and disaccharides, polysaccharide particles and yeast cells with boronate-containing copolymers (BCC) of N-acryloyl-m-aminophenylboronic acid (NAAPBA) with NN-dimethylacrylamide (DMAA) or N-isopropylacrylamide (NIPAM) was studied. The binding of saccharides to BCC of NIPAM resulted in a shift of its phase transition temperature (Delta T-p), which provided a quantitative measure for the complex formation. Among the sugars typical of non-reducing ends of glycoproteins the Delta T-p decreased in the order: N-acetylneuraminic acid > xylose approximate to galactose > mannose approximate to fucose >> N-acetylglucosamine. Strong specific adsorption of the BCC on the cross-linked agarose gel Sepharose CL-6B (15-30 mg/ml gel at pH 9.2) was registered. The copolymers adsorption was due to boronate-sugar interactions and decreased with pH. Multivalent interaction of the BCC with the agarose gel has been proven by liquid column chromatography exhibiting a weak reversible adsorption of NAAPBA and almost irreversible adsorption of DMAA-NAAPBA copolymer from 0.1 M sodium phosphate buffer, pH 7.9. The two studied BCCs could be completely desorbed from the gel by 0.1 M fructose in aqueous buffered media with pH from 7.5 to 9.2. In turn, the agarose particles and yeast cells were found to adhere to siliceous supports end-grafted with boronate-BCC of N,N-dimethylacrylamide at pH >= 7.5, due to the actions. Quantitative detachment of adhered particles or cells could be attained by addition of 20 mM or 100 mM fructose, respectively, in the pH range from 7.5 to 9.2. Affinity adhesion of micron-size carbohydrate particles to boronate-containing polymer brushes fixed on solid supports was considered as a model system suggesting a new approach to isolation and separation of living cells. Copyright (c) 2006 John Wiley & Sons, Ltd. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/397374
- author
- Ivanov, Alexander LU ; Galaev, Igor LU and Mattiasson, Bo LU
- organization
- publishing date
- 2006
- type
- Chapter in Book/Report/Conference proceeding
- publication status
- published
- subject
- keywords
- thermoresponsive polymers, biomimetic, binding constant, boronic acid, cell adhesion, cell, oligosaccharides, chemisorption, detachment, immobilization
- host publication
- Journal of Molecular Recognition
- volume
- 19
- issue
- 4
- pages
- 322 - 331
- publisher
- John Wiley & Sons Inc.
- conference name
- Bioaffinity 2005
- conference location
- Upsala, Sweden
- conference dates
- 2005-08-14 - 2005-08-18
- external identifiers
-
- wos:000239882300011
- scopus:33747246960
- ISSN
- 0952-3499
- 1099-1352
- DOI
- 10.1002/jmr.792
- language
- English
- LU publication?
- yes
- id
- 8c499693-a8bf-4d39-8f26-e4352625e37a (old id 397374)
- date added to LUP
- 2016-04-01 12:15:53
- date last changed
- 2024-10-10 03:24:20
@inproceedings{8c499693-a8bf-4d39-8f26-e4352625e37a,
abstract = {{Interaction of mono- and disaccharides, polysaccharide particles and yeast cells with boronate-containing copolymers (BCC) of N-acryloyl-m-aminophenylboronic acid (NAAPBA) with NN-dimethylacrylamide (DMAA) or N-isopropylacrylamide (NIPAM) was studied. The binding of saccharides to BCC of NIPAM resulted in a shift of its phase transition temperature (Delta T-p), which provided a quantitative measure for the complex formation. Among the sugars typical of non-reducing ends of glycoproteins the Delta T-p decreased in the order: N-acetylneuraminic acid > xylose approximate to galactose > mannose approximate to fucose >> N-acetylglucosamine. Strong specific adsorption of the BCC on the cross-linked agarose gel Sepharose CL-6B (15-30 mg/ml gel at pH 9.2) was registered. The copolymers adsorption was due to boronate-sugar interactions and decreased with pH. Multivalent interaction of the BCC with the agarose gel has been proven by liquid column chromatography exhibiting a weak reversible adsorption of NAAPBA and almost irreversible adsorption of DMAA-NAAPBA copolymer from 0.1 M sodium phosphate buffer, pH 7.9. The two studied BCCs could be completely desorbed from the gel by 0.1 M fructose in aqueous buffered media with pH from 7.5 to 9.2. In turn, the agarose particles and yeast cells were found to adhere to siliceous supports end-grafted with boronate-BCC of N,N-dimethylacrylamide at pH >= 7.5, due to the actions. Quantitative detachment of adhered particles or cells could be attained by addition of 20 mM or 100 mM fructose, respectively, in the pH range from 7.5 to 9.2. Affinity adhesion of micron-size carbohydrate particles to boronate-containing polymer brushes fixed on solid supports was considered as a model system suggesting a new approach to isolation and separation of living cells. Copyright (c) 2006 John Wiley & Sons, Ltd.}},
author = {{Ivanov, Alexander and Galaev, Igor and Mattiasson, Bo}},
booktitle = {{Journal of Molecular Recognition}},
issn = {{0952-3499}},
keywords = {{thermoresponsive polymers; biomimetic; binding constant; boronic acid; cell adhesion; cell; oligosaccharides; chemisorption; detachment; immobilization}},
language = {{eng}},
number = {{4}},
pages = {{322--331}},
publisher = {{John Wiley & Sons Inc.}},
title = {{Interaction of sugars, polysaccharides and cells with boronate-containing copolymers: from solution to polymer brushes}},
url = {{http://dx.doi.org/10.1002/jmr.792}},
doi = {{10.1002/jmr.792}},
volume = {{19}},
year = {{2006}},
}